PMID- 35669778 OWN - NLM STAT- MEDLINE DCOM- 20220608 LR - 20220716 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 13 DP - 2022 TI - Gpr174 Knockout Alleviates DSS-Induced Colitis via Regulating the Immune Function of Dendritic Cells. PG - 841254 LID - 10.3389/fimmu.2022.841254 [doi] LID - 841254 AB - BACKGROUND: Dysfunction of the immune system would disturb the intestinal homeostasis and lead to inflammatory bowel disease (IBD). Dendritic cells (DCs) help maintain intestinal homeostasis and immediately respond to pathogens or injuries once the mucosa barriers are destroyed during IBD. G protein-coupled receptors(GPR)174 is an essential regulator of immunity that is widely expressed in most immune cells, including DCs. However, the role of GPR174 in regulating the immune function of DC in colitis has not been investigated. METHODS: Dextran sodium sulfate (DSS) was administered to establish the mice colitis model. Data of weight, length of colon, disease activity index (DAI), and macroscopic scores were collected. The flow cytometry was used to detect the infiltrations of T cells and DCs, the mean fluorescence intensity (MFI) of CD80, CD86, CD40, and major histocompatibility complex-II (MHC-II). And T cells proliferataion was measured by carboxyfluorescein diacetate succinimidyl ester (CFSE). The expression of cytokines (tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-10 (IL-10), interferon-gamma (IFN-gamma), interleukin -4 (IL-4)) and GPR174 mRNA were measured by Elisa, quantitative polymerase chain reaction (qPCR), and immunofluorescence. RNA of bone-marrow-derived dendritic cells (BMDCs) was extracted for sequencing. Adoptive transfer of BMDCs was administrated intravenously. RESULTS: Gpr174(-/-) mice exposed to 3% DSS showed significant alleviation characterized by reduced loss of weight, more minor colon damage, and better DAI and macroscopic scores. The expression of pro-inflammatory cytokines (TNF-alpha, IL-6) decreased, while anti-inflammatory cytokine (IL-10) increased compared with WT mice. In vitro, Gpr174(-/-) BMDCs showed less maturity, with a declined expression of MHC-II, CD80, CD86 and reduced TNF-alpha, higher IL-10 after LPS stimulation. Gpr174(-/-) BMDCs were less capable of activating OT-II naive CD4(+) T cells than WT BMDCs and induced more Th0 cells to differentiate into Treg while less into Th1. Furthermore, the transcriptome sequencing analysis exhibited that Gpr174 participated in TNF-alpha (NF-kappaB) signaling, leukocyte transendothelial migration, and Th1/Th2 cell differentiation pathways. Adoptive transfer of Gpr174(-/-) BMDCs to WT mice ameliorated DSS-induced colitis. CONCLUSION: Our study indicated that GPR174 was involved in the pathogenesis of IBD by regulating the maturation of the dendritic cells to maintain immune homeostasis. TNF-alpha (NF-kappaB) signaling pathway, leukocyte transendothelial migration, and Th1/Th2 cell differentiation pathways may be the target pathway. CI - Copyright (c) 2022 Wei, Mu, Han, Chen, Kuang, Wu, Luo, Tong, Zhang, Yang and Song. FAU - Wei, Wei AU - Wei W AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Mu, Sucheng AU - Mu S AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Han, Yi AU - Han Y AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Chen, Yao AU - Chen Y AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Kuang, Zhongshu AU - Kuang Z AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Wu, Xingyue AU - Wu X AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Luo, Yue AU - Luo Y AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Tong, Chaoyang AU - Tong C AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Zhang, Yiqun AU - Zhang Y AD - Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Yang, Yilin AU - Yang Y AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Song, Zhenju AU - Song Z AD - Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Key Laboratory of Lung Inflammation and Injury, Shanghai, China. AD - Shanghai Institute of Infectious Disease and Biosecurity, School of Public Health, Fudan University, Shanghai, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220520 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Cytokines) RN - 0 (GPR174 protein, mouse) RN - 0 (Interleukin-6) RN - 0 (NF-kappa B) RN - 0 (Receptors, G-Protein-Coupled) RN - 0 (Tumor Necrosis Factor-alpha) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Animals MH - *Colitis/chemically induced/genetics MH - Cytokines/metabolism MH - Dendritic Cells MH - Disease Models, Animal MH - Immunity MH - *Inflammatory Bowel Diseases/chemically induced/metabolism MH - Interleukin-10/metabolism MH - Interleukin-6/metabolism MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - NF-kappa B/metabolism MH - Receptors, G-Protein-Coupled/genetics/metabolism MH - Tumor Necrosis Factor-alpha/metabolism PMC - PMC9164256 OTO - NOTNLM OT - GPR174 OT - T cell activation OT - dendritic cells OT - inflammatory bowel disease OT - intestinal barrier function COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/06/08 06:00 MHDA- 2022/06/09 06:00 PMCR- 2022/01/01 CRDT- 2022/06/07 02:45 PHST- 2021/12/22 00:00 [received] PHST- 2022/04/25 00:00 [accepted] PHST- 2022/06/07 02:45 [entrez] PHST- 2022/06/08 06:00 [pubmed] PHST- 2022/06/09 06:00 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2022.841254 [doi] PST - epublish SO - Front Immunol. 2022 May 20;13:841254. doi: 10.3389/fimmu.2022.841254. eCollection 2022.