PMID- 35670188
OWN - NLM
STAT- MEDLINE
DCOM- 20220620
LR  - 20220719
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 14
IP  - 23
DP  - 2022 Jun 16
TI  - Rapid and selective determination of 9 nitrosamines in biological samples using 
      ultra-high performance liquid chromatography-triple quadrupole linear ion trap 
      mass spectrometry.
PG  - 2249-2254
LID - 10.1039/d2ay00468b [doi]
AB  - A sensitive, selective and convenient method for the simultaneous determination 
      of 9 nitrosamines (NAs) in biological samples was developed using isotope 
      dilution ultra-high performance liquid chromatography-triple quadrupole linear 
      ion trap mass spectrometry (UPLC-QTRAP-MS). Multiple reaction 
      monitoring-information dependent acquisition-enhanced product ion (MRM-IDA-EPI) 
      scan mode was performed to eliminate false positive results, and the whole 
      detection procedure was characterized by less time consuming and simple sample 
      preparation. 9 NAs were separated through a T3 column with the gradient elution 
      of acetonitrile and water, and detected by UPLC-QTRAP-MS with an atmospheric 
      pressure chemical ionization (APCI) source in the positive mode. The quantitative 
      analysis was carried out via the isotope internal standard method with a matrix 
      calibration curve. Under the optimized conditions, good linearity for the 9 NAs 
      was achieved in the range of 0.2-20 mug L(-1) with correlation coefficients (r) 
      higher than >/=0.9991, and the limits of detection and limits of quantitation were 
      0.02-0.1 mug L(-1) (S/N = 3) and 0.06-0.3 mug L(-1) (S/N = 10), respectively. 
      Satisfactory recoveries ranging from 79.4% to 108.0% were obtained, and the 
      precision of the proposed method, indicated by the relative standard deviations 
      (RSDs), was 2.3-12.9%. The matrix effect study showed that NDMA, NMOR and NMEA 
      presented a matrix suppression effect, NDPHA displayed a matrix enhancement 
      effect, and the matrix effects of the other 5 analytes could be ignored. Real 
      application of the developed method in 13 urine and 24 plasma samples 
      demonstrated that NDBA, NPIP and NPYR occurred in both urine and plasma samples 
      with the concentration of 0.038-0.60 mug L(-1), while other NAs were not detected. 
      Such a method was sensitive and selective, and could be applied to the rapid 
      qualitative and quantitative analysis of the 9 NAs in biological samples.
FAU - Zhou, Hua
AU  - Zhou H
AD  - Quzhou Center for Disease Control and Prevention, Quzhou 324000, China.
FAU - Huang, Qin
AU  - Huang Q
AD  - Quzhou Center for Disease Control and Prevention, Quzhou 324000, China.
FAU - Wu, Xianglun
AU  - Wu X
AD  - Quzhou Center for Disease Control and Prevention, Quzhou 324000, China.
FAU - Zhan, Bindong
AU  - Zhan B
AD  - Quzhou Center for Disease Control and Prevention, Quzhou 324000, China.
FAU - Chen, Dongyang
AU  - Chen D
AUID- ORCID: 0000-0002-1657-1719
AD  - Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan 
      410005, China. dychen2008@126.com.
FAU - Lei, Meikang
AU  - Lei M
AD  - The Comprehensive Technology and Service Center of Quzhou Customs, Quzhou 324003, 
      China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan 
      410005, China. dychen2008@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220616
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
RN  - 0 (Nitrosamines)
SB  - IM
MH  - Atmospheric Pressure
MH  - Chromatography, High Pressure Liquid/methods
MH  - Mass Spectrometry/methods
MH  - *Nitrosamines
EDAT- 2022/06/08 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/07 04:33
PHST- 2022/06/08 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
PHST- 2022/06/07 04:33 [entrez]
AID - 10.1039/d2ay00468b [doi]
PST - epublish
SO  - Anal Methods. 2022 Jun 16;14(23):2249-2254. doi: 10.1039/d2ay00468b.