PMID- 35674215
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220809
IS  - 1348-0421 (Electronic)
IS  - 0385-5600 (Linking)
VI  - 66
IP  - 8
DP  - 2022 Aug
TI  - Detection and quantification of Epstein-Barr virus, cytomegalovirus, and human 
      herpesvirus-6 in stomach frozen tissue of chronic gastritis and gastric cancer 
      patients.
PG  - 379-385
LID - 10.1111/1348-0421.13013 [doi]
AB  - Human herpes viruses (HHVs) are among the most common infectious agents detected 
      in the gastrointestinal tract that might be involved in oncogenesis and other 
      gastrointestinal disorders. Although the link between the Epstein-Barr virus 
      (EBV) and gastric cancer (GC) has been established, the role of the viruses in 
      various stomach diseases remains unknown. The frequencies and viral copy number 
      of EBV, cytomegalovirus (CMV), and human herpesvirus 6 (HHV-6) among 50 gastric 
      cancer tumors and 105 chronic gastritis tissues were measured by quantitative 
      real-time PCR. In the tumor specimens and the adjacent normal tissues EBV was 
      found in 60% and 30.9%, CMV in 14% and 4.7%, and HHV-6 in 18%, and 14.2%, 
      respectively. The detection rate of EBV and CMV was found to be significantly 
      higher in tumor tissues relative to the adjacent normal tissues. Also, in chronic 
      gastritis, the frequency of EBV, CMV, and HHV-6 was 19%, 12.3%, and 15.2%, 
      respectively, compared with 16.4%, 1.1%, and 8.2% in their corresponding normal 
      tissues. Here, the CMV frequency was found to be significantly higher in 
      gastritis tissues relative to the adjacent normal tissues. Furthermore, viral 
      load in both gastric cancer and gastritis groups was higher in either tumor or 
      gastritis lesion compared with matched adjacent normal tissue. This study showed 
      a clear association between gastric cancer with both EBV and CMV. Meanwhile, 
      analyses revealed a strong association between the EBV, CMV, and HHV-6 viral 
      loads with gastritis (P = 0.0026, P < 0.0001, and P = 0.0405, respectively). Our 
      results suggest that these three viruses might contribute to the induction and 
      development the gastritis and gastric cancer.
CI  - (c) 2022 The Societies and John Wiley & Sons Australia, Ltd.
FAU - Sarshari, Behrang
AU  - Sarshari B
AD  - Department of Medical Virology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Mohebbi, Seyed Reza
AU  - Mohebbi SR
AUID- ORCID: 0000-0002-7020-7889
AD  - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, 
      Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Ravanshad, Mehrdad
AU  - Ravanshad M
AUID- ORCID: 0000-0003-1808-2481
AD  - Department of Medical Virology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Shahrokh, Shabnam
AU  - Shahrokh S
AD  - Research Center for Gastroenterology and Liver Diseases, Research Institute for 
      Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical 
      Siences, Tehran, Iran.
FAU - Aghdaei, Hamid Asadzadeh
AU  - Aghdaei HA
AD  - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, 
      Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Zali, Mohammad Reza
AU  - Zali MR
AD  - Research Center for Gastroenterology and Liver Diseases, Research Institute for 
      Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical 
      Siences, Tehran, Iran.
LA  - eng
GR  - 1408648/Tarbiat Modares University/
GR  - IRGLD-1038/Research Institute for Gastroenterology and Liver Diseases, Shahid 
      Beheshti University of Medical Sciences/
PT  - Journal Article
DEP - 20220629
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Cytomegalovirus/genetics
MH  - *Cytomegalovirus Infections
MH  - DNA, Viral/analysis
MH  - *Epstein-Barr Virus Infections/complications/diagnosis
MH  - *Gastritis/complications
MH  - Herpesvirus 4, Human/genetics
MH  - *Herpesvirus 6, Human/genetics
MH  - Humans
MH  - *Stomach Neoplasms/complications
OTO - NOTNLM
OT  - CMV
OT  - EBV
OT  - HHV-6
OT  - gastric cancer
OT  - gastritis
OT  - viral load
EDAT- 2022/06/09 06:00
MHDA- 2022/08/10 06:00
CRDT- 2022/06/08 06:34
PHST- 2022/05/13 00:00 [revised]
PHST- 2022/02/18 00:00 [received]
PHST- 2022/05/29 00:00 [accepted]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
PHST- 2022/06/08 06:34 [entrez]
AID - 10.1111/1348-0421.13013 [doi]
PST - ppublish
SO  - Microbiol Immunol. 2022 Aug;66(8):379-385. doi: 10.1111/1348-0421.13013. Epub 
      2022 Jun 29.