PMID- 35674921
OWN - NLM
STAT- MEDLINE
DCOM- 20220907
LR  - 20220907
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 389
IP  - 3
DP  - 2022 Sep
TI  - Tanshinone IIA increased amniotic fluid volume through down-regulating placental 
      AQPs expression via inhibiting the activity of GSK-3beta.
PG  - 547-558
LID - 10.1007/s00441-022-03646-5 [doi]
AB  - The mechanism of idiopathic oligohydramnios is still uncertain, and there is no 
      effective and targeted treatment for it. Placental aquaporins (AQPs) were 
      associated with idiopathic oligohydramnios. This study aimed to investigate the 
      effect of tanshinone IIA on amniotic fluid volume (AFV) and its underlying 
      molecular mechanisms related to placental AQPs (AQP1, AQP3, AQP8, AQP9). Results 
      showed that compared with the women with normal AFV, placental AQP1, AQP3, AQP8, 
      and AQP9 protein expressions were decreased in women with idiopathic 
      oligohydramnios. Immunohistochemistry revealed localization of AQP1, AQP3, AQP8, 
      and AQP9 mainly in trophoblast cells within labyrinth zone of mouse placenta. 
      Also, AQP1 was located in fetal vascular endothelial cells. Pregnant mice were 
      administered with tanshinone IIA (10 mg/kg or 50 mg/kg, n = 8, respectively) or 
      vehicle (n = 8) from 9.5 to 18.5 gestational day (GD). Tanshinone IIA markedly 
      increased the AFV in pregnant mice, without the effects on embryo numbers per 
      litter, atrophic embryo rate, fetal weight, and placental weight, as well as 
      increased the expressions of AQPs and inhibited the activity of GSK-3beta in mice 
      placenta. In JEG-3 cells, tanshinone IIA downregulated AQP1, AQP3, AQP8, AQP9 
      expressions and inhibited the activity of GSK-3beta. Activating GSK-3beta with MK-2206 
      eliminated these alterations. Thus, tanshinone IIA could increase AFV in pregnant 
      mice, possibly through downregulating placental AQP1, AQP3, AQP8, and AQP9 
      expression via inhibiting the activity of GSK-3beta. Tanshinone IIA may be optional 
      for the treatment of idiopathic oligohydramnios.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Shao, Hailing
AU  - Shao H
AD  - Department of Obstetrics and Gynecology, the Second Affiliated Hospital of 
      Wenzhou Medical University, 325027, Wenzhou, China.
FAU - Pan, Shuangjia
AU  - Pan S
AD  - Department of Obstetrics and Gynecology, the Second Affiliated Hospital of 
      Wenzhou Medical University, 325027, Wenzhou, China.
FAU - Lan, Yehui
AU  - Lan Y
AD  - Department of Obstetrics and Gynecology, the Second Affiliated Hospital of 
      Wenzhou Medical University, 325027, Wenzhou, China.
FAU - Chen, Xianjun
AU  - Chen X
AD  - Taizhou Women and Childrens Hospital of Wenzhou Medical University, Taizhou, 
      China.
FAU - Dai, Dongru
AU  - Dai D
AD  - Department of Obstetrics and Gynecology, The Wenzhou Third Clinical Institute 
      Affiliated To Wenzhou Medical University, Wenzhou, China.
FAU - Peng, Lingli
AU  - Peng L
AD  - Department of Obstetrics and Gynecology, The Wenzhou Third Clinical Institute 
      Affiliated To Wenzhou Medical University, Wenzhou, China.
FAU - Hua, Ying
AU  - Hua Y
AUID- ORCID: 0000-0003-2798-4479
AD  - Department of Obstetrics and Gynecology, the Second Affiliated Hospital of 
      Wenzhou Medical University, 325027, Wenzhou, China. wzfeyhy1015@126.com.
LA  - eng
GR  - 2021KY215/the Project of Zhejiang Province Medical and Health Science and 
      Technology Plan/
GR  - Y20210026/the Science and Technology Bureau of Wenzhou/
GR  - 2017-XK-A42/the Obstetrics and gynecology of combine traditional Chinese and 
      Western medicine of Zhejiang Province/
PT  - Journal Article
DEP - 20220608
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Abietanes)
RN  - 0 (Aquaporins)
RN  - 03UUH3J385 (tanshinone)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
SB  - IM
MH  - Abietanes
MH  - Amniotic Fluid/chemistry/metabolism
MH  - Animals
MH  - *Aquaporins/metabolism
MH  - Cell Line, Tumor
MH  - Endothelial Cells/metabolism
MH  - Female
MH  - Glycogen Synthase Kinase 3 beta/metabolism
MH  - Humans
MH  - Mice
MH  - *Oligohydramnios/metabolism
MH  - Placenta/metabolism
MH  - Pregnancy
OTO - NOTNLM
OT  - Aquaporin
OT  - Glycogen synthetic kinase 3beta
OT  - Oligohydramnios
OT  - Placenta
OT  - Tanshinone IIA
EDAT- 2022/06/09 06:00
MHDA- 2022/09/08 06:00
CRDT- 2022/06/08 11:20
PHST- 2021/10/28 00:00 [received]
PHST- 2022/05/27 00:00 [accepted]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/09/08 06:00 [medline]
PHST- 2022/06/08 11:20 [entrez]
AID - 10.1007/s00441-022-03646-5 [pii]
AID - 10.1007/s00441-022-03646-5 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2022 Sep;389(3):547-558. doi: 10.1007/s00441-022-03646-5. Epub 
      2022 Jun 8.