PMID- 35674941
OWN - NLM
STAT- MEDLINE
DCOM- 20220610
LR  - 20220824
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2517
DP  - 2022
TI  - Isolation of Candida auris in Clinical Specimens.
PG  - 3-20
LID - 10.1007/978-1-0716-2417-3_1 [doi]
AB  - Candida auris is a multidrug-resistant yeast causing healthcare-associated 
      outbreaks of blood stream infections worldwide. Currently, C. auris isolation and 
      identification is complicated by issues such as misidentification and long 
      turnaround time associated with application of commonly used diagnostic tools. 
      Based on phenotypic characteristics, differentiation of C. auris from related 
      Candida haemulonii complex spp. is problematic. Candida auris can be 
      misidentified using biochemical-based systems such as VITEK 2 YST, API 20C, BD 
      Phoenix yeast identification system, and MicroScan. C. auris growth at 42  degrees C and 
      in the presence of 10% NaCl helps in presumptive identification of this yeast 
      from related Candida haemulonii complex spp. A new CHROMagar Candida Plus agar 
      is an excellent alternative to current conventional mycological media for the 
      screening of patients colonized/infected with Candida auris. Matrix-assisted 
      laser desorption/ionization time-of-flight (MALDI-TOF) can differentiate C. auris 
      from other Candida species, but not all the reference databases included in 
      MALDI-TOF devices allow for detection. Currently, accurate identification of C. 
      auris can be performed using the updated FDA-approved libraries or "research 
      use-only" libraries. Molecular techniques have greatly enhanced the diagnosis of 
      C. auris. Sequencing of rDNA genetic loci, namely, internal transcribed spacer 
      and D1/D2 region of large subunit (LSU), and PCR/qPCR assays has successfully 
      been applied for identification of C. auris. Real-time PCR assays bear 
      incomparable potential of being the most efficient tool for high-throughput 
      screening of surveillance samples. If properly validated, they can deliver the 
      diagnostic result within several hours, since the DNA can be isolated directly 
      from the patient specimen without the need of obtaining a colony. In this chapter 
      we detailed the isolation of Candida auris from various clinical specimens and 
      its currently available identification methods and hitches.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Yadav, Anamika
AU  - Yadav A
AUID- ORCID: 0000-0002-1735-9904
AD  - Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest 
      Institute, University of Delhi, Delhi, India.
AD  - Department of Zoology, Ramjas College, University of Delhi, Delhi, India.
FAU - Singh, Ashutosh
AU  - Singh A
AUID- ORCID: 0000-0002-6807-7130
AD  - Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest 
      Institute, University of Delhi, Delhi, India. singh90.ashutosh@gmail.com.
FAU - Chowdhary, Anuradha
AU  - Chowdhary A
AUID- ORCID: 0000-0002-2028-7462
AD  - Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest 
      Institute, University of Delhi, Delhi, India.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antifungal Agents)
SB  - IM
MH  - Antifungal Agents
MH  - Candida/genetics
MH  - Candida auris
MH  - *Candidiasis/diagnosis
MH  - Humans
MH  - Saccharomyces cerevisiae
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
OTO - NOTNLM
OT  - CHROMagar Candida
OT  - Candida auris
OT  - Misidentification
OT  - Molecular identification
OT  - Phenotypic identification
EDAT- 2022/06/09 06:00
MHDA- 2022/06/11 06:00
CRDT- 2022/06/08 11:21
PHST- 2022/06/08 11:21 [entrez]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/06/11 06:00 [medline]
AID - 10.1007/978-1-0716-2417-3_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2517:3-20. doi: 10.1007/978-1-0716-2417-3_1.