PMID- 35674954
OWN - NLM
STAT- MEDLINE
DCOM- 20220610
LR  - 20230308
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2517
DP  - 2022
TI  - Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow 
      Cytometry and Fluorescence Microscopy.
PG  - 179-187
LID - 10.1007/978-1-0716-2417-3_14 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) of the fungal cell wall are 
      primary targets for the innate immune system of animals. Therefore, 
      characterizing PAMP exposure of fungal pathogens helps to elucidate how they 
      interact with their hosts at a molecular level. Fluorescent labelling can be used 
      to monitor exposure of multiple fungal cell wall PAMPs in a single experiment. 
      Here, we describe a protocol to simultaneously label chitin, mannan, and 
      beta-1,3-glucan in Candida auris to study these PAMPs by fluorescence microscopy and 
      allow high-throughput examination of their exposure by flow cytometry.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Pelletier, Chloe
AU  - Pelletier C
AUID- ORCID: 0000-0002-5581-3442
AD  - Institute of Medical Sciences (IMS), University of Aberdeen, Aberdeen, UK.
AD  - MRC Centre for Medical Mycology, Department of Biosciences, University of Exeter, 
      Exeter, UK.
FAU - Lorenz, Alexander
AU  - Lorenz A
AUID- ORCID: 0000-0003-1925-3713
AD  - Institute of Medical Sciences (IMS), University of Aberdeen, Aberdeen, UK. 
      a.lorenz@abdn.ac.uk.
LA  - eng
GR  - MR/V033417/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/P501955/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antifungal Agents)
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
SB  - IM
MH  - Animals
MH  - Antifungal Agents
MH  - *Candida auris
MH  - Cell Wall
MH  - Flow Cytometry
MH  - Microscopy, Fluorescence
MH  - *Pathogen-Associated Molecular Pattern Molecules
OTO - NOTNLM
OT  - Candida auris
OT  - Cell wall
OT  - Chitin
OT  - Flow cytometry
OT  - Fluorescence microscopy
OT  - Mannan
OT  - PAMPs
OT  - beta-1,3-Glucan
EDAT- 2022/06/09 06:00
MHDA- 2022/06/11 06:00
CRDT- 2022/06/08 11:21
PHST- 2022/06/08 11:21 [entrez]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/06/11 06:00 [medline]
AID - 10.1007/978-1-0716-2417-3_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2517:179-187. doi: 10.1007/978-1-0716-2417-3_14.