PMID- 35676456
OWN - NLM
STAT- MEDLINE
DCOM- 20221107
LR  - 20240213
IS  - 1873-4626 (Electronic)
IS  - 1091-255X (Linking)
VI  - 26
IP  - 9
DP  - 2022 Sep
TI  - Gut Barrier Disruption Secondary to Radiofrequency-Assisted Liver Parenchyma 
      Resection in a Porcine Model.
PG  - 1881-1889
LID - 10.1007/s11605-022-05370-5 [doi]
AB  - BACKGROUND: Radiofrequency-assisted liver resection (RF-LR) techniques minimize 
      intraoperative blood loss, while avoiding the Pringle maneuver. Both surgical 
      excision and radiofrequency ablation of liver parenchyma compromise gut barrier 
      function with subsequent bacterial translocation. The present study sought to 
      investigate in a porcine model the impact of two RF-LR techniques on the 
      integrity and inflammatory response of the gut barrier. METHODS: Twenty-four pigs 
      were subjected to either (a) partial hepatectomy (PH) employing the "sequential 
      coagulate-cut" technique using a monopolar electrode (SCC group), the one using 
      the bipolar Habib-4X device (group H), or the "crush-clamp" technique (group CC) 
      or (b) sham operation (group Sham). At 48-h post-operation, ileal tissue was 
      excised to be subjected to histopathologic examination, histomorphometric 
      analysis, and immunohistochemical assessment of the mitotic and apoptotic 
      activities and the expression of interleukin-6 (IL-6), tumor necrosis factor-alpha 
      (TNFalpha), and nuclear factor-kappaB (NFkappaBeta). RESULTS: Histopathologic score increased in 
      all PH groups, being higher in group SCC, while lower in group H. Villous height 
      decreased in group SCC only. Mitotic index decreased, while apoptotic index 
      increased in all PH groups. An increase in tissue expression score was noted for 
      IL-6 in group CC, for TNFalpha in all PH groups, being lower in group H compared to 
      group CC, and for NFkappaB in all PH groups. CONCLUSIONS: The Habib-4X technique for 
      liver resection proved to preserve the integrity of gut barrier, being less 
      injurious in the intestinal mucosa compared to the SCC and CC techniques.
CI  - (c) 2022. The Society for Surgery of the Alimentary Tract.
FAU - Ypsilantis, Petros
AU  - Ypsilantis P
AUID- ORCID: 0000-0002-7744-3809
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece. 
      pipsil@med.duth.gr.
FAU - Lambropoulou, Maria
AU  - Lambropoulou M
AD  - Laboratory of Histology and Embryology, School of Medicine, Democritus University 
      of Thrace, Alexandroupolis, Greece.
FAU - Karayiannakis, Anastasios
AU  - Karayiannakis A
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
FAU - Zacharoulis, Dimitrios
AU  - Zacharoulis D
AD  - Clinic of Surgery, School of Medicine, University of Thessaly, Larisa, Greece.
FAU - Passos, Ioannis
AU  - Passos I
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
FAU - Smyrlis, Christos
AU  - Smyrlis C
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
FAU - Charisis, Christos
AU  - Charisis C
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
FAU - Ypsilantis, Konstantinos
AU  - Ypsilantis K
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
FAU - Pitiakoudis, Michael
AU  - Pitiakoudis M
AD  - Laboratory of Experimental Surgery and Surgical Research, School of Medicine, 
      Democritus University of Thrace, Dragana, 68100, Alexandroupolis, Greece.
LA  - eng
GR  - 80216/Special Account for Research Funds of the Democritus University of Thrace, 
      Greece/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220608
PL  - Netherlands
TA  - J Gastrointest Surg
JT  - Journal of gastrointestinal surgery : official journal of the Society for Surgery 
      of the Alimentary Tract
JID - 9706084
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - *Catheter Ablation/adverse effects/methods
MH  - *Hepatectomy/adverse effects/methods
MH  - Interleukin-6
MH  - Liver/pathology/surgery
MH  - NF-kappa B
MH  - Swine
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - Gut barrier
OT  - Liver resection
OT  - Radiofrequency
EDAT- 2022/06/09 06:00
MHDA- 2022/09/24 06:00
CRDT- 2022/06/08 23:28
PHST- 2022/03/01 00:00 [received]
PHST- 2022/05/21 00:00 [accepted]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
PHST- 2022/06/08 23:28 [entrez]
AID - S1091-255X(23)05767-0 [pii]
AID - 10.1007/s11605-022-05370-5 [doi]
PST - ppublish
SO  - J Gastrointest Surg. 2022 Sep;26(9):1881-1889. doi: 10.1007/s11605-022-05370-5. 
      Epub 2022 Jun 8.