PMID- 35676724
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230916
IS  - 1749-8546 (Print)
IS  - 1749-8546 (Electronic)
IS  - 1749-8546 (Linking)
VI  - 17
IP  - 1
DP  - 2022 Jun 8
TI  - Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic 
      dermatitis in BALB/c mice and HaCaT cells.
PG  - 66
LID - 10.1186/s13020-022-00624-5 [doi]
LID - 66
AB  - BACKGROUND: Isatis tinctoria L (PLG) is a medicinal herb from the roots of Isatis 
      indigotica Fort (Family Cruciferae). Previous studies have shown that PLG has 
      anti-inflammatory and therapeutic effects against conditions such as acute and 
      chronic hepatitis, various respiratory inflammations, and cancer. The purpose of 
      this study was to define the pharmacological effects of PLG on inflammatory 
      reactions and skin hyperkeratosis, which are the main symptoms of atopic 
      dermatitis (AD), in vivo and in vitro. METHODS: For the AD in vivo experiment, 
      2,4-dinitrochlorobenzene (DNCB) induction and oral administration of PLG were 
      performed on male BALB/c mice for four weeks. For in vitro experiments, 
      keratinocytes were activated using TNF-alpha/IFN-gamma in cultured human keratinocyte 
      (HaCaT) cells. PLG inhibited inflammatory chemokine production and blocked the 
      nuclear translocation of NF-kappaB p65 in activated keratinocytes. RESULTS: As a 
      result of oral administration of PLG, dermis and epidermis thickening, as well as 
      eosinophil and mast cell infiltration, were attenuated in AD skin lesions. In 
      addition, the levels of immunoglobulin E (IgE), pro-inflammatory cytokines, and 
      the MAPK/NF-kappaB signaling pathway were decreased in serum and dorsal skin tissues. 
      Furthermore, PLG inhibited inflammatory chemokine production and blocked the 
      nuclear translocation of NF-kappaB p65 in activated keratinocytes. In addition, 
      epigoitrin and adenosine, the standard compounds of PLG, were identified as 
      candidate AD compounds. CONCLUSIONS: These results indicate that PLG is a potent 
      therapeutic agent for attenuating symptoms of AD.
CI  - (c) 2022. The Author(s).
FAU - Min, Ga-Yul
AU  - Min GY
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea.
FAU - Kim, Tae In
AU  - Kim TI
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea.
FAU - Kim, Ji-Hye
AU  - Kim JH
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea.
FAU - Cho, Won-Kyung
AU  - Cho WK
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea.
FAU - Yang, Ju-Hye
AU  - Yang JH
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea. jjuhye@kiom.re.kr.
FAU - Ma, Jin-Yeul
AU  - Ma JY
AD  - Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 
      Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea. jyma@kiom.re.kr.
LA  - eng
GR  - KSN1812101/Korea Institute of Oriental Medicine/
GR  - KSN2021230/Korea Institute of Oriental Medicine/
PT  - Journal Article
DEP - 20220608
PL  - England
TA  - Chin Med
JT  - Chinese medicine
JID - 101265109
PMC - PMC9175348
OTO - NOTNLM
OT  - Atopic dermatitis
OT  - BALB/c mice
OT  - HaCaT cells
OT  - Isatis tinctoria L
COIS- The authors declare that they have no competing interests.
EDAT- 2022/06/09 06:00
MHDA- 2022/06/09 06:01
PMCR- 2022/06/08
CRDT- 2022/06/08 23:42
PHST- 2022/01/05 00:00 [received]
PHST- 2022/05/28 00:00 [accepted]
PHST- 2022/06/08 23:42 [entrez]
PHST- 2022/06/09 06:00 [pubmed]
PHST- 2022/06/09 06:01 [medline]
PHST- 2022/06/08 00:00 [pmc-release]
AID - 10.1186/s13020-022-00624-5 [pii]
AID - 624 [pii]
AID - 10.1186/s13020-022-00624-5 [doi]
PST - epublish
SO  - Chin Med. 2022 Jun 8;17(1):66. doi: 10.1186/s13020-022-00624-5.