PMID- 35677051
OWN - NLM
STAT- MEDLINE
DCOM- 20220610
LR  - 20220716
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Enhanced T Cell Glucose Uptake Is Associated With Progression of Beta-Cell 
      Function in Type 1 Diabetes.
PG  - 897047
LID - 10.3389/fimmu.2022.897047 [doi]
LID - 897047
AB  - BACKGROUND: Abnormal intracellular glucose/fatty acid metabolism of T cells has 
      tremendous effects on their immuno-modulatory function, which is related to the 
      pathogenesis of autoimmune diseases. However, the association between the status 
      of intracellular metabolism of T cells and type 1 diabetes is unclear. This study 
      aimed to investigate the uptake of glucose and fatty acids in T cells and its 
      relationship with disease progression in type 1 diabetes. METHODS: A total of 86 
      individuals with type 1 diabetes were recruited to detect the uptake of glucose 
      and fatty acids in T cells. 2-NBDG uptake and expression of glucose transporter 1 
      (GLUT1); or BODIPY uptake and expression of carnitine palmitoyltransferase 
      1A(CPT1A) were used to assess the status of glucose or fatty acid uptake in T 
      cells. Patients with type 1 diabetes were followed up every 3-6 months for 36 
      months, the progression of beta-cell function was assessed using generalized 
      estimating equations, and survival analysis was performed to determine the status 
      of beta-cell function preservation (defined as 2-hour postprandial C-peptide >200 
      pmol/L). RESULTS: Patients with type 1 diabetes demonstrated enhanced 
      intracellular glucose uptake of T cells as indicated by higher 2NBDG uptake and 
      GLUT1 expression, while no significant differences in fatty acid uptake were 
      observed. The increased T cells glucose uptake is associated with lower C-peptide 
      and higher hemoglobin A1c levels. Notably, patients with low T cell glucose 
      uptake at onset maintained high levels of C-peptide within 36 months of the 
      disease course [fasting C-petite and 2-hour postprandial C-peptide are 60.6 
      (95%CI: 21.1-99.8) pmol/L and 146.3 (95%CI: 14.1-278.5) pmol/L higher 
      respectively], And they also have a higher proportion of beta-cell function 
      preservation during this follow-up period (P<0.001). CONCLUSIONS: Intracellular 
      glucose uptake of T cells is abnormally enhanced in type 1 diabetes and is 
      associated with beta-cell function and its progression.
CI  - Copyright (c) 2022 Tang, Zhong, Fan, Xie, Li and Li.
FAU - Tang, Rong
AU  - Tang R
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Zhong, Ting
AU  - Zhong T
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Fan, Li
AU  - Fan L
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Xie, Yuting
AU  - Xie Y
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Li, Juan
AU  - Li J
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Li, Xia
AU  - Li X
AD  - National Clinical Research Center for Metabolic Diseases, Key Laboratory of 
      Diabetes Immunology (Central South University), Ministry of Education, and 
      Department of Metabolism and Endocrinology, The Second Xiangya Hospital of 
      Central South University, Changsha, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220523
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (C-Peptide)
RN  - 0 (Fatty Acids)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Insulin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - C-Peptide/metabolism
MH  - *Diabetes Mellitus, Type 1/complications
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Fatty Acids
MH  - Glucose/metabolism
MH  - Glucose Transporter Type 1
MH  - Humans
MH  - Insulin/metabolism
MH  - T-Lymphocytes/metabolism
PMC - PMC9168918
OTO - NOTNLM
OT  - T cell
OT  - beta-cell function
OT  - cellular glucose uptake
OT  - immunometabolism
OT  - type 1 diabetes
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/06/10 06:00
MHDA- 2022/06/11 06:00
PMCR- 2022/01/01
CRDT- 2022/06/09 02:06
PHST- 2022/03/15 00:00 [received]
PHST- 2022/04/22 00:00 [accepted]
PHST- 2022/06/09 02:06 [entrez]
PHST- 2022/06/10 06:00 [pubmed]
PHST- 2022/06/11 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.897047 [doi]
PST - epublish
SO  - Front Immunol. 2022 May 23;13:897047. doi: 10.3389/fimmu.2022.897047. eCollection 
      2022.