PMID- 35681333
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 11
IP  - 11
DP  - 2022 May 28
TI  - Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic 
      Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and 
      MALDI-TOF/TOF Mass Spectrometry Imaging.
LID - 10.3390/foods11111583 [doi]
LID - 1583
AB  - Polygonum perfoliatum L. has a long history of medicinal and edible applications. 
      Studies have shown that it can significantly protect liver injury, but the 
      mechanism is unclear. The purpose of this study was to explore the protective 
      mechanism of P. perfoliatum on chronic alcoholic liver injury from the 
      perspective of lipid metabolism. After 8 weeks of alcohol exposure in male Wister 
      mice, the levels of aspartate aminotransferase (AST), alanine aminotransferase 
      (ALT) and alkaline phosphatase (ALP) in serum were significantly increased, and 
      the activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase 
      (ALDH) in liver were significantly decreased. Meanwhile, pathological changes of 
      liver tissue in mice were observed by histopathology. Then, Ultra-High 
      Performance Liquid Chromatography (UHPLC) QExactive Plus Mass Spectrometer 
      lipidomics and matrix-assisted laser desorption/ionization time-of-flight/time 
      -of-flight (MALDI-TOF/TOF) mass spectrometry imaging methods were established to 
      analyze lipid metabolism in mice. Ten different lipids were identified by 
      statistical analysis, including Fatty Acyls, Glycerophospholipids, Prenol lipids 
      and Sphingomyelins. After intervention with P. perfoliatum extracts at different 
      doses (25 to 100 mg/kg), levels of AST, ALT, ALP in serum, and activities of ADH 
      and ALDH in liver were significantly corrected. The hepatic cord structure was 
      clear, and the liver cells were closely arranged without other obvious 
      abnormalities. Non-target lipidomics analysis showed that P. perfoliatum extract 
      could regulate the metabolic disorders of the 10 different lipids caused by 
      continuous alcohol exposure. Pathway analysis suggested that the mechanism of P. 
      perfoliatum extract on chronic alcoholic liver injury may be related to the 
      regulation of linoleic acid and alpha-linolenic acid.
FAU - Chen, Huaguo
AU  - Chen H
AUID- ORCID: 0000-0002-1821-4088
AD  - Key Laboratory for Information System of Mountainous Areas and Protection of 
      Ecological Environment, Guizhou Normal University, Guiyang 550001, China.
AD  - Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of 
      Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang 550001, 
      China.
FAU - Peng, Lei
AU  - Peng L
AD  - Key Laboratory for Information System of Mountainous Areas and Protection of 
      Ecological Environment, Guizhou Normal University, Guiyang 550001, China.
AD  - Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of 
      Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang 550001, 
      China.
FAU - Zhao, Chao
AU  - Zhao C
AD  - Key Laboratory for Information System of Mountainous Areas and Protection of 
      Ecological Environment, Guizhou Normal University, Guiyang 550001, China.
AD  - Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of 
      Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang 550001, 
      China.
FAU - Cai, Zongwei
AU  - Cai Z
AD  - State Key Laboratory of Environmental and Biological Analysis, Department of 
      Chemistry, Hong Kong Baptist University, Hong Kong SAR 999077, China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Key Laboratory for Information System of Mountainous Areas and Protection of 
      Ecological Environment, Guizhou Normal University, Guiyang 550001, China.
AD  - Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of 
      Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang 550001, 
      China.
LA  - eng
GR  - 81860738/National Natural Science Foundation of China/
GR  - [2018]5769-27/Guizhou Normal University Academic New Seedling Cultivation and 
      Innovation Exploration Special Project/
PT  - Journal Article
DEP - 20220528
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC9179971
OTO - NOTNLM
OT  - Polygonum perfoliatum L.
OT  - chronic alcoholic liver injury
OT  - mass spectrometry imaging
OT  - non-target lipidomics
COIS- The authors declare no conflict to interest.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/11 06:01
PMCR- 2022/05/28
CRDT- 2022/06/10 01:07
PHST- 2022/04/24 00:00 [received]
PHST- 2022/05/13 00:00 [revised]
PHST- 2022/05/24 00:00 [accepted]
PHST- 2022/06/10 01:07 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/11 06:01 [medline]
PHST- 2022/05/28 00:00 [pmc-release]
AID - foods11111583 [pii]
AID - foods-11-01583 [pii]
AID - 10.3390/foods11111583 [doi]
PST - epublish
SO  - Foods. 2022 May 28;11(11):1583. doi: 10.3390/foods11111583.