PMID- 35681602
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 14
IP  - 11
DP  - 2022 May 25
TI  - Pilot Investigation on p75ICD Expression in Laryngeal Squamous Cell Carcinoma.
LID - 10.3390/cancers14112622 [doi]
LID - 2622
AB  - We investigated the p75 Neurotrophin Receptor (p75NTR) expression and cleavage 
      product p75NTR Intracellular Domain (p75ICD) as potential oncogenic and 
      metastatic markers in human Laryngeal Squamous Cell Carcinoma (LSCC). p75NTR is 
      highly expressed in Cancer Stem Cells (CSCs) of the laryngeal epithelia and it 
      has been proposed as a marker for stemness, cell migration, and chemo-resistance 
      in different squamous carcinomas. To investigate the clinical significance of 
      p75NTR cleavage products in solid tumors, full-length and cleaved p75NTR 
      expression was analyzed in laryngeal primary tumors from different-stage LSCC 
      patients, diagnosed at the Policlinico Umberto I Hospital. Molecular and 
      histological techniques were used to detect the expressions of p75NTR and p75ICD, 
      and ATP Binding Cassette Subfamily G Member 2 (ABCG2), a CSC marker. We found 
      regulated p75NTR cleavage during squamous epithelial tumor progression and tissue 
      invasion. Our preliminary investigation suggests p75ICD expression and 
      localization as possible features of tumorigenesis and metastaticity. Its 
      co-localization with ABCG2 in squamous cells in the parenchyma invaded by the 
      tumor formation allows us to hypothesize p75NTR and p75ICD roles in tumor 
      invasion and CSC spreading in LSCC patients. These data might represent a 
      starting point for a comprehensive analysis of p75NTR cleavage and of its 
      clinical relevance as a potential molecular LSCC signature, possibly helping 
      diagnosis, and improving prognosis and personalized therapy.
FAU - Triaca, Viviana
AU  - Triaca V
AUID- ORCID: 0000-0001-8290-7279
AD  - Institute of Biochemistry and Cell Biology, National Research Council (CNR), 
      International Campus A. Buzzati-Traverso, Monterotondo Scalo, 00015 Rome, Italy.
FAU - Fico, Elena
AU  - Fico E
AUID- ORCID: 0000-0003-1730-277X
AD  - Department of Sense Organs, Institute of Biochemistry and Cell Biology, National 
      Research Council (CNR), University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Rosso, Pamela
AU  - Rosso P
AD  - Department of Sense Organs, Institute of Biochemistry and Cell Biology, National 
      Research Council (CNR), University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Ralli, Massimo
AU  - Ralli M
AUID- ORCID: 0000-0001-8776-0421
AD  - Department of Sense Organs, University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Corsi, Alessandro
AU  - Corsi A
AUID- ORCID: 0000-0001-6896-4509
AD  - Department of Molecular Medicine, University of Rome La Sapienza, 00185 Rome, 
      Italy.
FAU - Severini, Cinzia
AU  - Severini C
AUID- ORCID: 0000-0002-6932-704X
AD  - Department of Sense Organs, Institute of Biochemistry and Cell Biology, National 
      Research Council (CNR), University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Crevenna, Alvaro
AU  - Crevenna A
AUID- ORCID: 0000-0002-5580-3711
AD  - Epigenetics and Neurobiology Unit, EMBL Rome, International Campus A. 
      Buzzati-Traverso, Monterotondo Scalo, 00015 Rome, Italy.
FAU - Agostinelli, Enzo
AU  - Agostinelli E
AUID- ORCID: 0000-0002-2508-8868
AD  - Department of Sense Organs, University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Rullo, Emma
AU  - Rullo E
AUID- ORCID: 0000-0003-4152-5025
AD  - Department of Molecular Medicine, University of Rome La Sapienza, 00185 Rome, 
      Italy.
FAU - Riminucci, Mara
AU  - Riminucci M
AUID- ORCID: 0000-0002-8643-9211
AD  - Department of Molecular Medicine, University of Rome La Sapienza, 00185 Rome, 
      Italy.
FAU - Colizza, Andrea
AU  - Colizza A
AUID- ORCID: 0000-0002-0639-806X
AD  - Department of Sense Organs, University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Polimeni, Antonella
AU  - Polimeni A
AUID- ORCID: 0000-0002-2679-7607
AD  - Department of Oral and Maxillo Facial Sciences, University of Rome La Sapienza, 
      00185 Rome, Italy.
FAU - Greco, Antonio
AU  - Greco A
AD  - Department of Sense Organs, University of Rome La Sapienza, 00185 Rome, Italy.
FAU - Tirassa, Paola
AU  - Tirassa P
AD  - Department of Sense Organs, Institute of Biochemistry and Cell Biology, National 
      Research Council (CNR), University of Rome La Sapienza, 00185 Rome, Italy.
LA  - eng
GR  - GrandiProgetti 2019/Sapienza University of Rome/
PT  - Journal Article
DEP - 20220525
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC9179539
OTO - NOTNLM
OT  - ABCG2
OT  - CSCs
OT  - LSCC
OT  - p75ICD
OT  - p75NTR
OT  - tumor invasion
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/11 06:01
PMCR- 2022/05/25
CRDT- 2022/06/10 01:08
PHST- 2022/04/14 00:00 [received]
PHST- 2022/05/19 00:00 [revised]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/06/10 01:08 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/11 06:01 [medline]
PHST- 2022/05/25 00:00 [pmc-release]
AID - cancers14112622 [pii]
AID - cancers-14-02622 [pii]
AID - 10.3390/cancers14112622 [doi]
PST - epublish
SO  - Cancers (Basel). 2022 May 25;14(11):2622. doi: 10.3390/cancers14112622.