PMID- 35683981
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20220716
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 14
IP  - 11
DP  - 2022 May 24
TI  - Long-Term High-Fat High-Fructose Diet Induces Type 2 Diabetes in Rats through 
      Oxidative Stress.
LID - 10.3390/nu14112181 [doi]
LID - 2181
AB  - Long-term consumption of a Western diet is a major cause of type 2 diabetes 
      mellitus (T2DM). However, the effects of diet on pancreatic structure and 
      function remain unclear. Rats fed a high-fat, high-fructose (HFHF) diet were 
      compared with rats fed a normal diet for 3 and 18 months. Plasma biochemical 
      parameters and inflammatory factors were used to reflect metabolic profile and 
      inflammatory status. The rats developed metabolic disorders, and the size of the 
      islets in the pancreas increased after 3 months of HFHF treatment but decreased 
      and became irregular after 18 months. Fasting insulin, C-peptide, proinsulin, and 
      intact proinsulin levels were significantly higher in the HFHF group than those 
      in the age-matched controls. Plasmatic oxidative parameters and nucleic acid 
      oxidation markers (8-oxo-Gsn and 8-oxo-dGsn) became elevated before inflammatory 
      factors, suggesting that the HFHF diet increased the degree of oxidative stress 
      before affecting inflammation. Single-cell RNA sequencing also verified that the 
      transcriptional level of oxidoreductase changed differently in islet 
      subpopulations with aging and long-term HFHF diet. We demonstrated that long-term 
      HFHF diet and aging-associated structural and transcriptomic changes that 
      underlie pancreatic islet functional decay is a possible underlying mechanism of 
      T2DM, and our study could provide new insights to prevent the development of 
      diet-induced T2DM.
FAU - Zhao, Yue
AU  - Zhao Y
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
AD  - Graduate School of Peking Union Medical College, Beijing 100730, China.
FAU - Wang, Qing-Yu
AU  - Wang QY
AUID- ORCID: 0000-0002-9352-0923
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
FAU - Zeng, Lv-Tao
AU  - Zeng LT
AD  - Peking University Fifth School of Clinical Medicine, Beijing Hospital/National 
      Center of Gerontology of National Health Commission, Beijing 100730, China.
FAU - Wang, Jing-Jing
AU  - Wang JJ
AD  - Department of Clinical Laboratory, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450066, China.
FAU - Liu, Zhen
AU  - Liu Z
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
AD  - Graduate School of Peking Union Medical College, Beijing 100730, China.
FAU - Fan, Guo-Qing
AU  - Fan GQ
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
AD  - Graduate School of Peking Union Medical College, Beijing 100730, China.
FAU - Li, Jin
AU  - Li J
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
FAU - Cai, Jian-Ping
AU  - Cai JP
AD  - The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of 
      Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 
      Hospital/National Center of Gerontology of National Health Commission, Beijing 
      100730, China.
AD  - Graduate School of Peking Union Medical College, Beijing 100730, China.
LA  - eng
GR  - No. 82170856/National Natural Science Foundation of China/
GR  - No. 2021-1-I2M-050/CAMS Innovation Fund for Medical Sciences/
GR  - 2018YFC2000300/National Key R&D Program of China/
PT  - Journal Article
DEP - 20220524
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 30237-26-4 (Fructose)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/etiology
MH  - Diet, High-Fat/adverse effects
MH  - Fructose/adverse effects
MH  - *Insulin Resistance
MH  - Oxidative Stress
MH  - Rats
PMC - PMC9182436
OTO - NOTNLM
OT  - Western dietary pattern
OT  - islets
OT  - metabolic disorders
OT  - pancreas
OT  - single-cell RNA sequencing analysis
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/14 06:00
PMCR- 2022/05/24
CRDT- 2022/06/10 01:21
PHST- 2022/04/01 00:00 [received]
PHST- 2022/05/13 00:00 [revised]
PHST- 2022/05/20 00:00 [accepted]
PHST- 2022/06/10 01:21 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/05/24 00:00 [pmc-release]
AID - nu14112181 [pii]
AID - nutrients-14-02181 [pii]
AID - 10.3390/nu14112181 [doi]
PST - epublish
SO  - Nutrients. 2022 May 24;14(11):2181. doi: 10.3390/nu14112181.