PMID- 35684398
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20220716
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 11
DP  - 2022 May 27
TI  - Isoquercitrin Attenuates Osteogenic Injury in MC3T3 Osteoblastic Cells and the 
      Zebrafish Model via the Keap1-Nrf2-ARE Pathway.
LID - 10.3390/molecules27113459 [doi]
LID - 3459
AB  - Isoquercitrin (IQ) widely exists in natural products, with a variety of 
      pharmacological activities. In this study, the anti-apoptotic and antioxidative 
      activities of IQ were evaluated. IQ showed protective activity against 2, 
      2'-azobis [2-methylpropionamidine] dihydrochloride (AAPH)-induced cell damage, as 
      well as a marked reduction in reactive oxygen species (ROS). The evidence of IQ 
      regulating Keap1-Nrf2-ARE and the mitochondrial-mediated Caspase 3 pathway were 
      found in the MC3T3 osteoblastic cell line. Furthermore, IQ significantly 
      decreased ROS production, apoptosis, and lipid peroxidation in AAPH-treated 72 h 
      post-fertilization (hpf) zebrafish, as observed via DCFH-DA, acridine orange 
      (AO), and a 1,3-bis(diphenylphosphino) propane (DPPP) probe, respectively. In 
      AAPH-treated 9 day post-fertilization (dpf) zebrafish, IQ strongly promoted 
      osteogenic development, with increased concentrations by calcein staining, 
      compared with the untreated group. In a molecular docking assay, among all signal 
      proteins, Keap1 showed the strongest affinity with IQ at -8.6 kcal/mol, which 
      might be the reason why IQ regulated the Keap1-Nrf2-ARE pathway in vitro and in 
      vivo. These results indicated that IQ promotes bone development and repairs bone 
      injury, which is valuable for the prevention and treatment of bone diseases.
FAU - Li, Xue
AU  - Li X
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Zhou, Dongyue
AU  - Zhou D
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Yang, Di
AU  - Yang D
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Fu, Yunhua
AU  - Fu Y
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Tao, Xingyu
AU  - Tao X
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Hu, Xuan
AU  - Hu X
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Dai, Yulin
AU  - Dai Y
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
FAU - Yue, Hao
AU  - Yue H
AD  - Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological 
      Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of 
      Chinese Medicine, Changchun 130117, China.
LA  - eng
GR  - 20210401065YY/Scientific and Technological Development Program of Jilin province 
      of China/
GR  - 20210204046YY/Scientific and Technological Development Program of Jilin province 
      of China/
PT  - Journal Article
DEP - 20220527
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0YX10VRV6J (isoquercitrin)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Kelch-Like ECH-Associated Protein 1/metabolism
MH  - Molecular Docking Simulation
MH  - *NF-E2-Related Factor 2/metabolism
MH  - Oxidative Stress
MH  - Quercetin/analogs & derivatives
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
MH  - *Zebrafish/metabolism
PMC - PMC9182080
OTO - NOTNLM
OT  - antioxidant
OT  - isoquercitrin
OT  - osteogenic development
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/14 06:00
PMCR- 2022/05/27
CRDT- 2022/06/10 01:24
PHST- 2022/04/05 00:00 [received]
PHST- 2022/05/21 00:00 [revised]
PHST- 2022/05/26 00:00 [accepted]
PHST- 2022/06/10 01:24 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/05/27 00:00 [pmc-release]
AID - molecules27113459 [pii]
AID - molecules-27-03459 [pii]
AID - 10.3390/molecules27113459 [doi]
PST - epublish
SO  - Molecules. 2022 May 27;27(11):3459. doi: 10.3390/molecules27113459.