PMID- 35684411
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20230308
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 11
DP  - 2022 May 27
TI  - Transcriptome Profiling of HCT-116 Colorectal Cancer Cells with RNA Sequencing 
      Reveals Novel Targets for Polyphenol Nano Curcumin.
LID - 10.3390/molecules27113470 [doi]
LID - 3470
AB  - Colorectal cancer is one of the leading causes of cancer-related deaths 
      worldwide. The gemini nanoparticle formulation of polyphenolic curcumin 
      significantly inhibits the viability of cancer cells. However, the molecular 
      mechanisms and pathways underlying its toxicity in colon cancer are unclear. 
      Here, we aimed to uncover the possible novel targets of gemini curcumin 
      (Gemini-Cur) on colorectal cancer and related cellular pathways. After confirming 
      the cytotoxic effect of Gemini-Cur by MTT and apoptotic assays, RNA sequencing 
      was employed to identify differentially expressed genes (DEGs) in HCT-116 cells. 
      On a total of 3892 DEGs (padj < 0.01), 442 genes showed a log2 FC >|2| (including 
      244 upregulated and 198 downregulated). Gene ontology (GO) enrichment analysis 
      was performed. Protein-protein interaction (PPI) and gene-pathway networks were 
      constructed by using STRING and Cytoscape. The pathway analysis showed that 
      Gemini-Cur predominantly modulates pathways related to the cell cycle. The gene 
      network analysis revealed five central genes, namely GADD45G, ATF3, BUB1B, CCNA2 
      and CDK1. Real-time PCR and Western blotting analysis confirmed the significant 
      modulation of these genes in Gemini-Cur-treated compared to non-treated cells. In 
      conclusion, RNA sequencing revealed novel potential targets of curcumin on cancer 
      cells. Further studies are required to elucidate the molecular mechanism of 
      action of Gemini-Cur regarding the modulation of the expression of hub genes.
FAU - Azeez, Hewa Jalal
AU  - Azeez HJ
AD  - Department of Biology, School of Natural Sciences, University of Tabriz, Tabriz 
      51368, Iran.
FAU - Neri, Francesco
AU  - Neri F
AD  - Life Sciences and Systems Biology Department, University of Torino, 10124 Torino, 
      Italy.
FAU - Hosseinpour Feizi, Mohammad Ali
AU  - Hosseinpour Feizi MA
AD  - Life Sciences and Systems Biology Department, University of Torino, 10124 Torino, 
      Italy.
FAU - Babaei, Esmaeil
AU  - Babaei E
AUID- ORCID: 0000-0002-1603-5166
AD  - Department of Biology, School of Natural Sciences, University of Tabriz, Tabriz 
      51368, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220527
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Polyphenols)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - *Colonic Neoplasms
MH  - Computational Biology
MH  - *Curcumin/pharmacology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - HCT116 Cells
MH  - Humans
MH  - Polyphenols/pharmacology
MH  - Protein Interaction Maps
MH  - Sequence Analysis, RNA
MH  - Transcriptome
PMC - PMC9182402
OTO - NOTNLM
OT  - PPI network
OT  - RNA sequencing
OT  - colorectal cancer
OT  - differentially expressed genes
OT  - gemini curcumin
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/14 06:00
PMCR- 2022/05/27
CRDT- 2022/06/10 01:24
PHST- 2022/05/01 00:00 [received]
PHST- 2022/05/24 00:00 [revised]
PHST- 2022/05/24 00:00 [accepted]
PHST- 2022/06/10 01:24 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/05/27 00:00 [pmc-release]
AID - molecules27113470 [pii]
AID - molecules-27-03470 [pii]
AID - 10.3390/molecules27113470 [doi]
PST - epublish
SO  - Molecules. 2022 May 27;27(11):3470. doi: 10.3390/molecules27113470.