PMID- 35684457
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20220716
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 11
DP  - 2022 May 30
TI  - Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous 
      Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis 
      of Pheochromocytomas and Paragangliomas.
LID - 10.3390/molecules27113520 [doi]
LID - 3520
AB  - A novel dual-template magnetic molecularly imprinted polymer (MMIP) was 
      synthesized to extract normetanephrine (NMN), metanephrine (MN) and 
      3-methoxytyramine (3-MT) from spot urine samples. As the adsorbent of dispersive 
      solid-phase extraction (d-SPE), the MMIP was prepared using dopamine and MN as 
      dual templates, methacrylic acid as the functional monomer, ethylene glycol 
      dimethacrylate as the crosslinking reagent and magnetic nanoparticles as the 
      magnetic core. NMN, MN, 3-MT and creatinine (Cr) in spot urine samples were 
      selectively enriched by d-SPE and detected by HPLC-fluorescence 
      detection/ultraviolet detection. The peak area (A) ratios of NMN, MN and 3-MT to 
      Cr were used for the diagnosis of pheochromocytomas and paragangliomas (PPGLs). 
      The results showed that the adsorption efficiencies of MMIP for target analytes 
      were all higher than 89.0%, and the coefficient variation precisions of 
      intra-assay and inter-assay for the analytes were within 4.9% and 6.3%, 
      respectively. The recoveries of the analytes were from 93.2% to 112.8%. The MMIP 
      was still functional within 14 days and could be reused at least seven times. The 
      d-SPE and recommended solid-phase extraction (SPE) were both used to pretreat 
      spot urine samples from 18 PPGLs patients and 22 healthy controls. The 
      correlation coefficients of A(NMN)/A(Cr) and A(MN)/A(Cr) between d-SPE and SPE 
      were both higher than 0.95. In addition, the areas under the receiver operator 
      curves for spot urine A(NMN)/A(Cr), A(MN)/A(Cr) and plasma free NMN and MN were 
      0.975, 0.773 and 0.990, 0.821, respectively, indicating the two methods had the 
      similar performances. The d-SPE method took only 20 min, which was effective in 
      clinical application.
FAU - Zeng, Hongyu
AU  - Zeng H
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Zhang, Xiaoqing
AU  - Zhang X
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Zhen, Qianna
AU  - Zhen Q
AD  - Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, China.
FAU - He, Yifan
AU  - He Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, China.
FAU - Wang, Haoran
AU  - Wang H
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Zhu, Yang
AU  - Zhu Y
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Sun, Qi
AU  - Sun Q
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Ding, Min
AU  - Ding M
AD  - Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School 
      of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
LA  - eng
GR  - cstc2019jcyj-msxmX0103, cstc2020jcyj-msxmX0141/Natural Science Foundation of 
      Chongqing, China/
PT  - Journal Article
DEP - 20220530
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Molecularly Imprinted Polymers)
RN  - 0J45DE6B88 (Normetanephrine)
RN  - 5001-33-2 (Metanephrine)
RN  - AYI8EX34EU (Creatinine)
RN  - JCH2767EDP (3-methoxytyramine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - *Adrenal Gland Neoplasms/diagnosis
MH  - Creatinine
MH  - Dopamine/analogs & derivatives
MH  - Humans
MH  - Magnetic Phenomena
MH  - Metanephrine/urine
MH  - *Molecular Imprinting/methods
MH  - Molecularly Imprinted Polymers
MH  - Normetanephrine/urine
MH  - *Paraganglioma/diagnosis/urine
MH  - *Pheochromocytoma/diagnosis
PMC - PMC9182035
OTO - NOTNLM
OT  - 3-methoxytyramine
OT  - PPGLs diagnosis
OT  - magnetic molecularly imprinted polymer
OT  - metanephrine
OT  - normetanephrine
OT  - spot urine
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/06/11 06:00
MHDA- 2022/06/14 06:00
PMCR- 2022/05/30
CRDT- 2022/06/10 01:24
PHST- 2022/05/12 00:00 [received]
PHST- 2022/05/27 00:00 [revised]
PHST- 2022/05/28 00:00 [accepted]
PHST- 2022/06/10 01:24 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/05/30 00:00 [pmc-release]
AID - molecules27113520 [pii]
AID - molecules-27-03520 [pii]
AID - 10.3390/molecules27113520 [doi]
PST - epublish
SO  - Molecules. 2022 May 30;27(11):3520. doi: 10.3390/molecules27113520.