PMID- 35686354
OWN - NLM
STAT- MEDLINE
DCOM- 20220919
LR  - 20220919
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 36
IP  - 9
DP  - 2022 Sep
TI  - Mitoquinone mitigates paraquat-induced A549 lung epithelial cell injury by 
      promoting MFN1/MFN2-mediated mitochondrial fusion.
PG  - e23127
LID - 10.1002/jbt.23127 [doi]
AB  - Paraquat (PQ) poisoning often leads to severe lung injuries, in which the 
      mitochondria damage plays a critical role. Mitoquinone (MitoQ), a newly designed 
      mitochondria-targeted antioxidant, has been proved for its benefit in 
      mitochondria protection. However, the role of MitoQ in PQ-induced lung injury 
      remains unclear. Thus, this study was performed to investigate the effect of 
      MitoQ on PQ-induced lung injury and its underlying mechanisms. Our work showed 
      that PQ caused the inhibition of A549 lung epithelial cell viability in a 
      dose-dependent manner, while MitoQ remarkably mitigated the PQ-induced cell 
      viability suppression. Besides this, PQ-mediated apoptosis of A549 cells was 
      significantly attenuated by MitoQ, as indicated by the TUNEL assay and 
      mitochondria membrane potential assay. Moreover, the intracellular reactive 
      oxygen species (ROS) production was also dramatically suppressed when cotreated 
      MitoQ with PQ. This could be ascribed to enhanced mitochondrial fusion mediated 
      by Mitofusin 1 (MFN1)/Mitofusin 2 (MFN2), because MitoQ preserved mitochondrial 
      network integrity, as reflected by MitoTracker staining, and MitoQ also increased 
      the expression of MFN1/MFN2 in A549 cells after PQ treatment. Our data suggested 
      MitoQ mitigated PQ-induced lung epithelial cell injury by promoting 
      MFN1/MFN2-mediated mitochondrial fusion, and MitoQ might be a potential candidate 
      drug for the treatment of PQ-induced lung injury.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Liu, Chao
AU  - Liu C
AUID- ORCID: 0000-0002-0315-2195
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Sun, Zhaorui
AU  - Sun Z
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Wang, Mengmeng
AU  - Wang M
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Yang, Zhizhou
AU  - Yang Z
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Ren, Yi
AU  - Ren Y
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Han, Xiaoqin
AU  - Han X
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Yao, Mengya
AU  - Yao M
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
FAU - Nie, Shinan
AU  - Nie S
AD  - Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, PR China.
LA  - eng
GR  - 2020M683718/China Postdoctoral Science Foundation/
GR  - BK20190247/National Natural Science Foundation of Jiangsu Province/
GR  - BK20211136/National Natural Science Foundation of Jiangsu Province/
GR  - 82102311/National Natural Science Foundation of China/
GR  - 82172182/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220609
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Antioxidants)
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 1339-63-5 (Ubiquinone)
RN  - 47BYS17IY0 (mitoquinone)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (MFN2 protein, human)
RN  - EC 3.6.5.- (Mfn1 protein, human)
RN  - PLG39H7695 (Paraquat)
SB  - IM
MH  - A549 Cells
MH  - Antioxidants/pharmacology
MH  - GTP Phosphohydrolases/pharmacology
MH  - Humans
MH  - Lung/metabolism
MH  - *Lung Injury
MH  - Mitochondrial Dynamics
MH  - Mitochondrial Membrane Transport Proteins
MH  - Mitochondrial Proteins
MH  - Organophosphorus Compounds
MH  - *Paraquat/toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Ubiquinone/analogs & derivatives
OTO - NOTNLM
OT  - A549 cells
OT  - lung injury
OT  - mitochondrial fusion
OT  - mitoquinone
OT  - paraquat
EDAT- 2022/06/11 06:00
MHDA- 2022/09/20 06:00
CRDT- 2022/06/10 03:03
PHST- 2022/04/16 00:00 [revised]
PHST- 2021/10/11 00:00 [received]
PHST- 2022/05/29 00:00 [accepted]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/09/20 06:00 [medline]
PHST- 2022/06/10 03:03 [entrez]
AID - 10.1002/jbt.23127 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2022 Sep;36(9):e23127. doi: 10.1002/jbt.23127. Epub 2022 
      Jun 9.