PMID- 35686528
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20220716
IS  - 1862-1783 (Electronic)
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 23
IP  - 6
DP  - 2022 Jun 15
TI  - LncRNA-m18as1 competitively binds with miR-18a-5p to regulate 
      follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary 
      pituitary cells.
PG  - 502-514
LID - 1673-1581(2022)06-0502-13 [pii]
LID - 10.1631/jzus.B2101052 [doi]
AB  - Long noncoding RNAs (lncRNAs) are expressed in different species and different 
      tissues, and perform different functions, but little is known about their 
      involvement in the synthesis or secretion of follicle-stimulating hormone (FSH). 
      In general, we have revealed lncRNA‍‒‍microRNA (miRNA)‍‒‍‍messenger RNA (mRNA) 
      interactions that may play important roles in rat primary pituitary cells. In 
      this study, a new lncRNA was identified for the first time. First, we analyzed 
      the gene expression of lncRNA-m18as1 in different tissues and different stages by 
      reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and 
      observed the localization of lncRNA-m18as1 with fluorescence in situ 
      hybridization, which indicated that this lncRNA was distributed mainly in the 
      cytoplasm. Next, we used RT-qPCR and enzyme-linked immunosorbent assay (ELISA) to 
      analyze the regulation of FSH synthesis and secretion after overexpression or 
      knockdown of lncRNA-m18as1 and found that lncRNA-m18as1 was positively correlated 
      with FSH synthesis and secretion. In addition, mothers against decapentaplegic 
      homolog 2 (Smad2) was highly expressed in our sequencing results. We also 
      screened miR-18a-5p from our sequencing results as a miRNA that may bind to 
      lncRNA-m18as1 and Smad2. We used RNA immunoprecipitation-qPCR (RIP-qPCR) and/or 
      dual luciferase assays to confirm that lncRNA-m18as1 interacted with miR-18a-5p 
      and miR-18a-5p interacted with Smad2. Fluorescence in situ hybridization (FISH) 
      showed that lncRNA-m18as1 and miR-18a-5p were localized mainly in the cytoplasm. 
      Finally, we determined the relationship among lncRNA-m18as1, miR-18a-5p, and the 
      Smad2/3 pathway. Overall, we found that lncRNA-m18as1 acts as a molecular sponge 
      of miR-18a-5p to regulate the synthesis and secretion of FSH through the Smad2/3 
      pathway.
FAU - Zhang, Weidi
AU  - Zhang W
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Ren, Wenzhi
AU  - Ren W
AD  - Jilin Provincial Model Animal Engineering Research Center, Jilin University, 
      Changchun 130062, China.
FAU - Han, Dongxu
AU  - Han D
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Zhao, Guokun
AU  - Zhao G
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Wang, Haoqi
AU  - Wang H
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Guo, Haixiang
AU  - Guo H
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Zheng, Yi
AU  - Zheng Y
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Ji, Zhonghao
AU  - Ji Z
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China.
FAU - Gao, Wei
AU  - Gao W
AD  - Department of Laboratory Animals, College of Animal Sciences, Jilin University, 
      Changchun 130062, China. gaowei81@jlu.edu.cn.
FAU - Yuan, Bao
AU  - Yuan B
AD  - Jilin Provincial Model Animal Engineering Research Center, Jilin University, 
      Changchun 130062, China. gaowei81@jlu.edu.cn, yuan_bao@jlu.edu.cn.
LA  - eng
GR  - 31872349/the National Natural Science Foundation of China/
PT  - Journal Article
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Follicle Stimulating Hormone/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Rats
PMC - PMC9198230
OTO - NOTNLM
OT  - Competitive endogenous RNA (ceRNA)
OT  - Follicle-stimulating hormone (FSH)
OT  - Long noncoding RNA (lncRNA)
OT  - MicroRNA (miRNA)
OT  - Mothers against decapentaplegic homolog 2/3 (Smad2/3)
EDAT- 2022/06/11 06:00
MHDA- 2022/06/14 06:00
PMCR- 2022/06/15
CRDT- 2022/06/10 04:52
PHST- 2022/06/10 04:52 [entrez]
PHST- 2022/06/11 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/06/15 00:00 [pmc-release]
AID - 1673-1581(2022)06-0502-13 [pii]
AID - 1673-1581（2022）06-0502-13 [pii]
AID - 10.1631/jzus.B2101052 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2022 Jun 15;23(6):502-514. doi: 10.1631/jzus.B2101052.