PMID- 3569126
OWN - NLM
STAT- MEDLINE
DCOM- 19870601
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 120
IP  - 5
DP  - 1987 May
TI  - The use of radioimmunoassay to compare the tissue and subcellular distributions 
      of neurotensin and neuromedin N in the cat.
PG  - 2092-100
AB  - A RIA for neuromedin N (NMN) was developed using 125I-labeled NMN and rabbit 
      antisera raised against a synthetic NMN coupled to NH2-enriched 
      putrescine-thyroglobulin. One of the antisera (TG-B), which cross-reacted less 
      than 3% with the related substances, neurotensin (NT), Lys8,Asn9-NT8-13, and 
      xenopsin, was used to compare the distribution of immunoreactive NMN (iNMN) in 
      the cat to that of immunoreactive NT (iNT). Although both iNMN and iNT were found 
      primarily in the mucosa of the ileum, brain, pituitary, and adrenal, the ratio of 
      the two activities varied, depending upon the tissue. Feline iNMN from brain, 
      intestine, and adrenal was indistinguishable from synthetic NMN by HPLC. In 
      contrast, extracts of stomach and pancreas contained primarily NMN-related 
      cross-reacting substances, the concentrations of which increased up to 500-fold 
      within minutes of extraction in aqueous acid. Since these peptides were also 
      present to some extent in acid/acetone extracts, they appear to be endogenous 
      variants of NMN stored in precursor forms which could be activated by 
      acid-proteases. During sucrose gradient centrifugation of isotonic homogenates of 
      feline brain, intestine, and adrenal, iNMN and iNT comigrated in association with 
      synaptosome-like and vesicle-like particles, as identified by electron 
      microscopy. These results demonstrate the presence of a peptide similar to 
      porcine NMN in extracts of feline brain, adrenal, and small intestine and its 
      association with signaling vesicles. In addition, the ability of gastric and 
      pancreatic extracts to generate variants of NMN in large quantity is described.
FAU - Carraway, R E
AU  - Carraway RE
FAU - Mitra, S P
AU  - Mitra SP
LA  - eng
GR  - AI-19736/AI/NIAID NIH HHS/United States
GR  - AM-28565/AM/NIADDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Peptide Fragments)
RN  - 102577-25-3 (neuromedin N)
RN  - 39379-15-2 (Neurotensin)
SB  - IM
MH  - Adrenal Glands/analysis/ultrastructure
MH  - Animals
MH  - Brain/ultrastructure
MH  - Brain Chemistry
MH  - Cats
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Intestinal Mucosa/analysis/ultrastructure
MH  - Microscopy, Electron
MH  - Neurotensin/*analysis
MH  - Pancreas/analysis
MH  - Peptide Fragments/*analysis
MH  - Pituitary Gland/analysis
MH  - Radioimmunoassay
MH  - Stomach/analysis
MH  - Subcellular Fractions/analysis
MH  - Tissue Distribution
EDAT- 1987/05/01 00:00
MHDA- 1987/05/01 00:01
CRDT- 1987/05/01 00:00
PHST- 1987/05/01 00:00 [pubmed]
PHST- 1987/05/01 00:01 [medline]
PHST- 1987/05/01 00:00 [entrez]
AID - 10.1210/endo-120-5-2092 [doi]
PST - ppublish
SO  - Endocrinology. 1987 May;120(5):2092-100. doi: 10.1210/endo-120-5-2092.