PMID- 35693138
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2008-126X (Print)
IS  - 2228-7442 (Electronic)
IS  - 2008-126X (Linking)
VI  - 12
IP  - 6
DP  - 2021 Nov-Dec
TI  - Minocycline Enhance the Restorative Ability of Olfactory Ensheathing Cells by the 
      Upregulation of BDNF and GDNF Expression After Spinal Cord Injury.
PG  - 777-788
LID - 10.32598/bcn.2021.1727.1 [doi]
AB  - INTRODUCTION: Spinal Cord Injury (SCI) is a global public health issue that 
      results in extensive neuronal degeneration, axonal and myelin loss, and severe 
      functional deficits. Neurotrophic factors are a potential treatment for reducing 
      secondary damage, promoting axon growth; they are responsible for inducing 
      myelination after injury. Olfactory Ensheathing Cells (OECs) and minocycline have 
      promoted locomotor function after SCI. The present study investigated the 
      neuroprotective effects of combined treatment with minocycline and OECs on spinal 
      cord injury related to Brain-Derived Neurotrophic Factor (BDNF) and Glial Derived 
      Neurotrophic Factor (GDNF) expressions after SCI. METHODS: Adult female rats were 
      used to experimental SCI by weight compression method. Rats received an 
      intraperitoneal minocycline injection (90 mg/kg) immediately after SCI and 24 h 
      after injury. OECs were transplanted one week after the injury. The hindlimb 
      function was assessed using Basso Beattie Bresnahan (BBB) locomotor rating scale 
      and Electromyography (EMG). After 5 weeks, the spinal cord segment centered at 
      the injury site was removed for histopathological analysis. Immunohistological 
      and western blot assays were performed to observe the expression of NeuN, BDNF, 
      GDNF, and Myelin Basic Protein (MBP). RESULTS: SCI induced the loss of locomotor 
      function with decreased BDNF and GDNF expressions in the injury site. 
      Minocycline+OECs increased the score of the BBB locomotor scale and increased 
      spared tissue in the injury site. Immunohistochemical results suggested that NeuN 
      expression significantly increased in the minocycline+OECs group than other 
      groups. Moreover, electromyography amplitude in treated rats was increased 
      compared to the control group. BDNF, GDNF, and MBP expressions and the number of 
      ventral motor neurons increased further by minocycline+OECs in SCI rats. 
      CONCLUSION: The present study provides evidence that minocycline may facilitate 
      recovery of locomotor function by OECs by increasing BDNF and GDNF expressions 
      following SCI. HIGHLIGHTS: Combined treatment with Minocycline and OECs increased 
      the locomotor function.The results showed that BDNF and GDNF expression increased 
      by combined treatment with minocycline and OECs. PLAIN LANGUAGE SUMMARY: This 
      study examined the effect of combined treatment with minocycline and olfactory 
      ensheathing cell on the BDNF and GDNF expression after spinal cord injury model 
      in rat. The results showed that injection of minocycline before transplantation 
      of OECs enhances expression of neurotrophic factors that lead to an appropriate 
      environment for transplanted OECs and increases neuronal survival that promotes 
      tissue sparing and functional recovery.
CI  - Copyright(c) 2021 Iranian Neuroscience Society.
FAU - Pourkhodadad, Soheila
AU  - Pourkhodadad S
AUID- ORCID: 0000-0003-1550-0350
AD  - Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, 
      Iran.
FAU - Oryan, Shahrbanoo
AU  - Oryan S
AUID- ORCID: 0000-0001-5259-9156
AD  - Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, 
      Iran.
FAU - Hadipour, Mohammad Mehdi
AU  - Hadipour MM
AUID- ORCID: 0000-0001-7441-2292
AD  - Neuroscience Research Center, Baqiyatallah University of Medical Sciences, 
      Tehran, Iran.
FAU - Kaka, Gholamreza
AU  - Kaka G
AUID- ORCID: 0000-0001-8599-6123
AD  - Neuroscience Research Center, Baqiyatallah University of Medical Sciences, 
      Tehran, Iran.
FAU - Sadraie, Seyed Homayoon
AU  - Sadraie SH
AUID- ORCID: 0000-0001-6955-8035
AD  - Department of Anatomy, School of Medicine, Baqiyatallah University of Medical 
      Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20211101
PL  - Iran
TA  - Basic Clin Neurosci
JT  - Basic and clinical neuroscience
JID - 101575211
PMC - PMC9168820
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Electromyography
OT  - Glial derived neurotrophic factor
OT  - Minocycline
OT  - Myelin basic protein
OT  - Olfactory ensheathing cells
OT  - Spinal cord injury
COIS- Conflict of interest The authors declared no conflict of interest.
EDAT- 2022/06/14 06:00
MHDA- 2022/06/14 06:01
PMCR- 2021/11/01
CRDT- 2022/06/13 03:11
PHST- 2019/03/03 00:00 [received]
PHST- 2020/04/10 00:00 [revised]
PHST- 2020/04/27 00:00 [accepted]
PHST- 2022/06/13 03:11 [entrez]
PHST- 2022/06/14 06:00 [pubmed]
PHST- 2022/06/14 06:01 [medline]
PHST- 2021/11/01 00:00 [pmc-release]
AID - bcn-12-777 [pii]
AID - 10.32598/bcn.2021.1727.1 [doi]
PST - ppublish
SO  - Basic Clin Neurosci. 2021 Nov-Dec;12(6):777-788. doi: 10.32598/bcn.2021.1727.1. 
      Epub 2021 Nov 1.