PMID- 35693421
OWN - NLM
STAT- MEDLINE
DCOM- 20220614
LR  - 20220716
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 28
DP  - 2022
TI  - Protective effect of Glutaredoxin 1 against oxidative stress in lens epithelial 
      cells of age-related nuclear cataracts.
PG  - 70-82
AB  - PURPOSE: Glutaredoxin 1 (Grx1) is a key antioxidant protein that catalyzes 
      disulfide redox reactions. In this study, we investigated the expression and 
      protective effect of Grx1 against oxidative stress in nuclear cataracts. METHODS: 
      Human anterior capsule membrane samples were obtained from the eyes of cataract 
      patients (experimental group) and non-cataractous (control group) donors. The 
      levels of Grx1 protein and mRNA expression were investigated. The human lens 
      epithelial (HLE) cell line SRA 01/04 was transfected with Grx1-containing plasmid 
      or Grx1 small interfering RNA, and cultured under H(2)O(2) treatment, mimicking 
      oxidative stress conditions. Cell counts, clone formation, cell apoptosis, cell 
      cycle, and levels of oxidized glutathione disulfide and cellular reactive oxygen 
      species (ROS) were evaluated and quantified. RESULTS: Protein and mRNA transcript 
      levels of Grx1 were significantly lower in the human anterior capsule membrane of 
      the age-related nuclear (ARN) cataract group than in the control group. Grx1 
      overexpression protected HLE cells from H(2)O(2)-induced oxidative damage, 
      including alleviating G1 phase arrest, promoting cell proliferation, reducing 
      cell apoptosis, and decreasing intracellular ROS generation. Furthermore, 
      extracellular-signal-regulated kinase (ERK) phosphorylation in the human anterior 
      capsule membrane of ARN patients was higher in the experimental group than in the 
      control group. Grx1 overexpression reduced the levels of oxidized glutathione 
      disulfide and the phosphorylation of ERK. The administration of an ERK 
      phosphorylation inhibitor, PD98059, induced antioxidant effects in Grx1-silenced 
      cells. CONCLUSIONS: Grx1 expression is downregulated in the human anterior 
      capsule membrane of ARN patients, accompanied by an increase in ERK 
      phosphorylation. Thus, Grx1 can protect HLE cells against oxidative stress.
CI  - Copyright (c) 2022 Molecular Vision.
FAU - Fan, Qi
AU  - Fan Q
AD  - Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan 
      University, Shanghai, China.
AD  - NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, 
      Chinese Academy of Medical Sciences, Shanghai, China.
AD  - Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
AD  - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
FAU - Li, Dan
AU  - Li D
AD  - Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan 
      University, Shanghai, China.
AD  - NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, 
      Chinese Academy of Medical Sciences, Shanghai, China.
AD  - Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
AD  - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
FAU - Zhao, Zhennan
AU  - Zhao Z
AD  - Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan 
      University, Shanghai, China.
AD  - NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, 
      Chinese Academy of Medical Sciences, Shanghai, China.
AD  - Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
AD  - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
FAU - Jiang, Yongxiang
AU  - Jiang Y
AD  - Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan 
      University, Shanghai, China.
AD  - NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, 
      Chinese Academy of Medical Sciences, Shanghai, China.
AD  - Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
AD  - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
FAU - Lu, Yi
AU  - Lu Y
AD  - Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan 
      University, Shanghai, China.
AD  - NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, 
      Chinese Academy of Medical Sciences, Shanghai, China.
AD  - Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
AD  - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220519
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Antioxidants)
RN  - 0 (Glutaredoxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - ULW86O013H (Glutathione Disulfide)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Apoptosis
MH  - *Cataract/genetics/metabolism
MH  - Epithelial Cells/metabolism
MH  - Glutaredoxins/genetics/metabolism
MH  - Glutathione Disulfide/metabolism/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - *Lens, Crystalline/metabolism
MH  - Oxidative Stress
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/metabolism
PMC - PMC9122476
EDAT- 2022/06/14 06:00
MHDA- 2022/06/15 06:00
PMCR- 2022/01/01
CRDT- 2022/06/13 03:15
PHST- 2021/03/21 00:00 [received]
PHST- 2022/05/17 00:00 [accepted]
PHST- 2022/06/13 03:15 [entrez]
PHST- 2022/06/14 06:00 [pubmed]
PHST- 2022/06/15 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 8 [pii]
PST - epublish
SO  - Mol Vis. 2022 May 19;28:70-82. eCollection 2022.