PMID- 35693844
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2155-5435 (Print)
IS  - 2155-5435 (Electronic)
VI  - 8
IP  - 9
DP  - 2018 Sep 7
TI  - O(2) Reduction by Biosynthetic Models of Cytochrome c Oxidase: Insights into Role 
      of Proton Transfer Residues from Perturbed Active Sites Models of CcO.
PG  - 8915-8924
LID - 10.1021/acscatal.8b02240 [doi]
AB  - Myoglobin based biosynthetic models of perturbed cytochrome c oxidase (CcO) 
      active site are reconstituted, in situ, on electrodes where glutamate residues 
      are systematically introduced in the distal site of the heme/Cu active site 
      instead of a tyrosine residue. These biochemical electrodes show efficient 
      4e(-)/4H(+) reduction with turnover rates and numbers more than 10(7) M(-1) s(-1) 
      and 10(4), respectively. The H(2)O/D(2)O isotope effects of these series of 
      crystallographically characterized mutants bearing zero, one, and two glutamate 
      residues near the heme Cu active site of these perturbed CcO mimics are 16, 4, 
      and 2, respectively. In situ SERRS-RDE data indicate complete change in the 
      rate-determining step as proton transfer residues are introduced near the active 
      site. The high selectivity for 4e(-)/4H(+) O(2) reduction and systematic 
      variation of KSIE demonstrate the dominant role of proton transfer residues on 
      the isotope effect on rate and rate-determining step of O(2) reduction.
FAU - Mukherjee, Sohini
AU  - Mukherjee S
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Kolkata 700032, India.
FAU - Mukherjee, Manjistha
AU  - Mukherjee M
AUID- ORCID: 0000-0001-6550-6262
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Kolkata 700032, India.
FAU - Mukherjee, Arnab
AU  - Mukherjee A
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, Champaign, 
      Illinois 61801, United States.
FAU - Bhagi-Damodaran, Ambika
AU  - Bhagi-Damodaran A
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, Champaign, 
      Illinois 61801, United States.
FAU - Lu, Yi
AU  - Lu Y
AUID- ORCID: 0000-0003-1221-6709
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign, Champaign, 
      Illinois 61801, United States.
FAU - Dey, Abhishek
AU  - Dey A
AUID- ORCID: 0000-0002-9166-3349
AD  - Department of Inorganic Chemistry, Indian Association for the Cultivation of 
      Science, Kolkata 700032, India.
LA  - eng
GR  - R01 GM062211/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20180815
PL  - United States
TA  - ACS Catal
JT  - ACS catalysis
JID - 101562209
PMC - PMC9186439
MID - NIHMS1812113
OTO - NOTNLM
OT  - O2 reduction reaction
OT  - biosynthetic model
OT  - cytochrome c oxidase
OT  - kinetic isotope effect
OT  - kinetic rate constant
OT  - proton transfer residues
COIS- The authors declare no competing financial interest.
EDAT- 2018/09/07 00:00
MHDA- 2018/09/07 00:01
PMCR- 2022/06/10
CRDT- 2022/06/13 03:21
PHST- 2022/06/13 03:21 [entrez]
PHST- 2018/09/07 00:00 [pubmed]
PHST- 2018/09/07 00:01 [medline]
PHST- 2022/06/10 00:00 [pmc-release]
AID - 10.1021/acscatal.8b02240 [doi]
PST - ppublish
SO  - ACS Catal. 2018 Sep 7;8(9):8915-8924. doi: 10.1021/acscatal.8b02240. Epub 2018 
      Aug 15.