PMID- 35696262
OWN - NLM
STAT- MEDLINE
DCOM- 20220707
LR  - 20230614
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 94
IP  - 26
DP  - 2022 Jul 5
TI  - Simultaneous Multiplexed Imaging of Biomolecules in Transgenic Mouse Brain 
      Tissues Using Mass Spectrometry Imaging: A Multi-omic Approach.
PG  - 9297-9305
LID - 10.1021/acs.analchem.2c00676 [doi]
AB  - The importance of multi-omic-based approaches to better understand diverse 
      pathological mechanisms including neurodegenerative diseases has emerged. Spatial 
      information can be of great help in understanding how biomolecules interact 
      pathologically and in elucidating target biomarkers for developing therapeutics. 
      While various analytical methods have been attempted for imaging-based 
      biomolecule analysis, a multi-omic approach to imaging remains challenging due to 
      the different characteristics of biomolecules. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) is a powerful tool due to its sensitivity, chemical 
      specificity, and high spatial resolution in visualizing chemical information in 
      cells and tissues. In this paper, we suggest a new strategy to simultaneously 
      obtain the spatial information of various kinds of biomolecules that includes 
      both labeled and label-free approaches using ToF-SIMS. The enzyme-assisted 
      labeling strategy for the targets of interest enables the sensitive and specific 
      imaging of large molecules such as peptides, proteins, and mRNA, a task that has 
      been, to date, difficult for any MS analysis. Together with the strength of the 
      analytical performance of ToF-SIMS in the label-free tissue imaging of small 
      biomolecules, the proposed strategy allows one to simultaneously obtain 
      integrated information of spatial distribution of metabolites, lipids, peptides, 
      proteins, and mRNA at a high resolution in a single measurement. As part of the 
      suggested strategy, we present a sample preparation method suitable for MS 
      imaging. Because a comprehensive method to examine the spatial distribution of 
      multiple biomolecules in tissues has remained elusive, our strategy can be a 
      useful tool to support the understanding of the interactions of biomolecules in 
      tissues as well as pathological mechanisms.
FAU - Le, Minh-Uyen Thi
AU  - Le MT
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, Korea.
AD  - Department of Nano Science, University of Science and Technology (UST), Daejeon 
      34113, Korea.
FAU - Shon, Hyun Kyong
AU  - Shon HK
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, Korea.
FAU - Nguyen, Hong-Phuong
AU  - Nguyen HP
AD  - Department of Biomedical Science, Inha University College of Medicine, Incheon 
      22332, Korea.
FAU - Lee, Chul-Ho
AU  - Lee CH
AD  - Laboratory Animal Resource Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Kim, Kyoung-Shim
AU  - Kim KS
AD  - Laboratory Animal Resource Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Na, Hee-Kyung
AU  - Na HK
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, Korea.
FAU - Lee, Tae Geol
AU  - Lee TG
AUID- ORCID: 0000-0002-6674-4147
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, Korea.
AD  - Department of Nano Science, University of Science and Technology (UST), Daejeon 
      34113, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220613
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain
MH  - Mice
MH  - Mice, Transgenic
MH  - *Peptides
MH  - RNA, Messenger
MH  - *Spectrometry, Mass, Secondary Ion/methods
PMC - PMC9261934
COIS- The authors declare no competing financial interest.
EDAT- 2022/06/14 06:00
MHDA- 2022/07/08 06:00
PMCR- 2023/06/13
CRDT- 2022/06/13 11:52
PHST- 2022/06/14 06:00 [pubmed]
PHST- 2022/07/08 06:00 [medline]
PHST- 2022/06/13 11:52 [entrez]
PHST- 2023/06/13 00:00 [pmc-release]
AID - 10.1021/acs.analchem.2c00676 [doi]
PST - ppublish
SO  - Anal Chem. 2022 Jul 5;94(26):9297-9305. doi: 10.1021/acs.analchem.2c00676. Epub 
      2022 Jun 13.