PMID- 35697078
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20221005
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 418
IP  - 1
DP  - 2022 Sep 1
TI  - PLIN2 promotes HCC cells proliferation by inhibiting the degradation of HIF1alpha.
PG  - 113244
LID - S0014-4827(22)00237-3 [pii]
LID - 10.1016/j.yexcr.2022.113244 [doi]
AB  - PLIN2 has been found to be dysregulated in several human malignancies, which 
      influences cancer progression. However, the roles of PLIN2 in regulating 
      hepatocellular carcinoma (HCC) progression are still unclear. Here, we revealed 
      that PLIN2 was frequently upregulated in HCC cells and tissues, and increased 
      PLIN2 expression was associated with poor prognosis outcomes in HCC. In HCC 
      cells, overexpressing PLIN2 promoted cell proliferation, PLIN2-deficiency 
      inhibited cell vitality. Mechanistically, silencing of PLIN2 expression 
      downregulated hypoxia inducible factor 1-alpha (HIF1alpha) expression and this 
      downregulation in turn inhibited the targeting genes of HIF1alpha. Furthermore, we 
      found that PLIN2 stabilized and retarded the degradation of the HIF1alpha through 
      autophagy-lysosomal pathway by inhibiting AMPK/ULK1. Collectively, we clarified 
      the carcinogenic role of PLIN2 in HCC and suggested a prognostic biomarker for 
      diagnosis and clinical therapy in the future.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Liu, Wuguang
AU  - Liu W
AD  - Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, 
      The Second Hospital of Dalian Medical University, Dalian, 116027, China; CAS Key 
      Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
FAU - Liu, Xiaolong
AU  - Liu X
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Oncology, The Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, 116027, China.
FAU - Ling, Ting
AU  - Ling T
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Chen, Di
AU  - Chen D
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Otkur, Wuxiyar
AU  - Otkur W
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Zhao, Hailong
AU  - Zhao H
AD  - Department of General Surgery, People's Hospital of Huangyuan County, Huangyuan 
      County, Xining, 812100, China.
FAU - Ma, Ming
AU  - Ma M
AD  - Department of General Surgery, People's Hospital of Huangyuan County, Huangyuan 
      County, Xining, 812100, China.
FAU - Ma, Kexin
AU  - Ma K
AD  - Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, 
      The Second Hospital of Dalian Medical University, Dalian, 116027, China.
FAU - Dong, Bing
AU  - Dong B
AD  - Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, 
      The Second Hospital of Dalian Medical University, Dalian, 116027, China.
FAU - Yang, Zexuan
AU  - Yang Z
AD  - Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, 
      The Second Hospital of Dalian Medical University, Dalian, 116027, China.
FAU - Piao, Hai-Long
AU  - Piao HL
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China. Electronic address: hpiao@dicp.ac.cn.
FAU - Liang, Rui
AU  - Liang R
AD  - Department of General Surgery, Shenzhen University General Hospital/Shenzhen 
      University Clinical Medical Academy, Shenzhen, 518055, China; Carson 
      International Cancer Research Centre, Shenzhen University School of Medicine, 
      Shenzhen, 518055, China. Electronic address: lr9311038@yahoo.com.
FAU - Dong, Chengyong
AU  - Dong C
AD  - Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, 
      The Second Hospital of Dalian Medical University, Dalian, 116027, China. 
      Electronic address: dongchengy@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220610
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (PLIN2 protein, human)
RN  - 0 (Perilipin-2)
SB  - IM
MH  - Autophagy/genetics
MH  - *Carcinoma, Hepatocellular/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
MH  - *Liver Neoplasms/pathology
MH  - Perilipin-2/metabolism
OTO - NOTNLM
OT  - AMPKalpha
OT  - Autophagy
OT  - HCC
OT  - HIF1alpha
OT  - PLIN2
EDAT- 2022/06/14 06:00
MHDA- 2022/07/20 06:00
CRDT- 2022/06/13 19:22
PHST- 2020/12/11 00:00 [received]
PHST- 2022/05/22 00:00 [revised]
PHST- 2022/06/04 00:00 [accepted]
PHST- 2022/06/14 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/06/13 19:22 [entrez]
AID - S0014-4827(22)00237-3 [pii]
AID - 10.1016/j.yexcr.2022.113244 [doi]
PST - ppublish
SO  - Exp Cell Res. 2022 Sep 1;418(1):113244. doi: 10.1016/j.yexcr.2022.113244. Epub 
      2022 Jun 10.