PMID- 35705467
OWN - NLM
STAT- MEDLINE
DCOM- 20220617
LR  - 20220628
IS  - 0253-3758 (Print)
IS  - 0253-3758 (Linking)
VI  - 50
IP  - 6
DP  - 2022 Jun 24
TI  - [Gene expression signature analysis of peripheral blood mononuclear cells from 
      patients with for high altitude pulmonary hypertension and value for potential 
      drug selection].
PG  - 577-584
LID - 10.3760/cma.j.cn112148-20220328-00215 [doi]
AB  - Objective: To investigate the gene expression characteristics of peripheral blood 
      mononuclear cells from patients with high altitude pulmonary hypertension (HAPH) 
      in Naxi residents living in Lijiang, Yunnan, and to explore the underlying 
      pathogenesis and value for potential drug selection. Methods: This is a 
      case-control study. Six patients with HPAH (HPAH group) and 4 normal subjects 
      (control group) were selected from the Naxi residents who originally lived in 
      Lijiang, Yunnan Province. The general clinical data of the two groups were 
      collected, and the related indexes of pulmonary artery pressure were collected. 
      Peripheral blood mononuclear cells of the subjects were collected for RNA 
      sequencing. The differences on gene expression, regulatory network of 
      transcription factors and drug similarity between the two groups were compared. 
      The results were compared with the public data of idiopathic pulmonary arterial 
      hypertension (IPAH). Biological processes and signal pathways were analyzed and 
      compared between HPAH and IPAH patients. Results: The age of 6 patients with HAPH 
      was (68.1+/-8.3) years old, and there were 2 males (2/6). The age of 4 subjects in 
      the control group was (62.3+/-10.9) years old, and there were 2 males (2/4). 
      Tricuspid regurgitation velocity, tricuspid pressure gradient and pulmonary 
      systolic pressure in HAPH group were significantly higher than those in control 
      group (all P<0.05). The results of RNA sequencing showed that compared with the 
      control group, 174 genes were significantly upregulated and 169 genes were 
      downregulated in peripheral blood mononuclear cells of HAPH group. These 
      differentially expressed genes were associated with 220 biological processes, 52 
      molecular functions and 23 cell components. A total of 21 biological processes 
      and 2 signal pathways differed between HPAH and IPAH groups, most of which were 
      related to inflammation and immune response. ZNF384, SP1 and STAT3 were selected 
      as highly correlated transcription factors by transcription factor prediction 
      analysis. Trichostatin A and vorinostat were screened out as potential drugs for 
      the treatment of HAPH by drug similarity analysis. Conclusions: There are 
      significant differences in gene expression in peripheral blood monocytes between 
      HAPH patients and normal population, and inflammation and immune dysfunction are 
      the main pathogenic factors. Trichostatin A and Vorinostat are potential drugs 
      for the treatment of HAPH.
FAU - Wu, X H
AU  - Wu XH
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Chen, Z R
AU  - Chen ZR
AD  - Department of Cardiology, First Affiliated Hospital of Guizhou Medical 
      University, Guiyang 550001, China.
FAU - He, Z Y
AU  - He ZY
AD  - Department of Cardiology, People's Hospital of Yulong, Lijiang 674100, China.
FAU - Dong, Y
AU  - Dong Y
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Yang, Y
AU  - Yang Y
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Zhao, Q Y
AU  - Zhao QY
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Yang, W
AU  - Yang W
AD  - Department of Cardiology, Luohe Center Hospital, Luohe 462005, China.
FAU - Wang, L Y
AU  - Wang LY
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Fu, C J
AU  - Fu CJ
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Yang, X D
AU  - Yang XD
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
FAU - Liu, H
AU  - Liu H
AD  - Department of Cardiology, First Affiliated Hospital, Dali University, Dali 
      671000, China.
LA  - chi
GR  - 81560073, 81760085, 81960085/National Natural Science Foundation of China/
PT  - Journal Article
PL  - China
TA  - Zhonghua Xin Xue Guan Bing Za Zhi
JT  - Zhonghua xin xue guan bing za zhi
JID - 7910682
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Transcription Factors)
RN  - 3X2S926L3Z (trichostatin A)
RN  - 58IFB293JI (Vorinostat)
RN  - Pulmonary edema of mountaineers
SB  - IM
MH  - Aged
MH  - Altitude
MH  - Altitude Sickness/genetics
MH  - Case-Control Studies
MH  - China
MH  - Familial Primary Pulmonary Hypertension/genetics
MH  - Humans
MH  - Hydroxamic Acids/pharmacology/therapeutic use
MH  - *Hypertension, Pulmonary/drug therapy/genetics
MH  - Inflammation
MH  - *Leukocytes, Mononuclear/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Transcription Factors
MH  - *Transcriptome/genetics
MH  - Vorinostat/pharmacology/therapeutic use
EDAT- 2022/06/16 06:00
MHDA- 2022/06/18 06:00
CRDT- 2022/06/15 22:10
PHST- 2022/06/15 22:10 [entrez]
PHST- 2022/06/16 06:00 [pubmed]
PHST- 2022/06/18 06:00 [medline]
AID - 10.3760/cma.j.cn112148-20220328-00215 [doi]
PST - ppublish
SO  - Zhonghua Xin Xue Guan Bing Za Zhi. 2022 Jun 24;50(6):577-584. doi: 
      10.3760/cma.j.cn112148-20220328-00215.