PMID- 35709597
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220621
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 220
DP  - 2022 Jul 15
TI  - Mechanistic insight into co-metabolic dechlorination of hexachloro-1,3-butadiene 
      in Dehalococcoides.
PG  - 118725
LID - S0043-1354(22)00678-9 [pii]
LID - 10.1016/j.watres.2022.118725 [doi]
AB  - Hexachloro-1,3-butadiene (HCBD) as one of emerging persistent organic pollutants 
      (POPs) poses potential risk to human health and ecosystems. 
      Organohalide-respiring bacteria (OHRB)-mediated reductive dehalogenation 
      represents a promising strategy to remediate HCBD-contaminated sites. 
      Nonetheless, information on the HCBD-dechlorinating OHRB and their dechlorination 
      pathways remain unknown. In this study, both in vivo and in vitro experiments, as 
      well as quantum chemical calculation, were employed to successfully identify and 
      characterize the reductive dechlorination of HCBD by Dehalococcoides. Results 
      showed that some Dehalococcoides extensively dechlorinated HCBD to 
      (E)-1,2,3-tri-CBD via (E)-1,1,2,3,4-penta-CBD and (Z,E)-1,2,3,4-tetra-CBD in a 
      co-metabolic way. Both qPCR and 16S rRNA gene amplicon sequencing analyses 
      suggested that the HCBD-dechlorinating Dehalococcoides coupled their cell growth 
      with dechlorination of perchloroethene (PCE), rather than HCBD. The in vivo and 
      in vitro ATPase assays indicated >/=78.89% decrease in ATPase activity upon HCBD 
      addition, which suggested HCBD inhibition on ATPase-mediated energy harvest and 
      provided rationality on the Dehalococcoides-mediated co-metabolic dechlorination 
      of HCBD. Interestingly, dehalogenation screening of organohalides with the 
      HCBD-dechlorinating enrichment cultures showed that debromination of 
      bromodichloromethane (BDCM) was active in the in vitro RDase assays but 
      non-active in the in vivo experiments. Further in vitro assays of hydrogenase 
      activity suggested that significant inhibition of BDCM on the hydrogenase 
      activity could block electron derivation from H(2) for consequent reduction of 
      organohalides in the in vivo experiments. Therefore, our results provided 
      unprecedented insight into metabolic, co-metabolic and RDase-active-only 
      dehalogenation of varied organohalides by specific OHRB, which could guide future 
      screening of OHRB for remediation of sites contaminated by HCBD and other POPs.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Shen, Rui
AU  - Shen R
AD  - Environmental Microbiomics Research Center, School of Environmental Science and 
      Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology, Southern Marine Science and Engineering 
      Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou, 510006 China.
FAU - Zhang, Shangwei
AU  - Zhang S
AD  - Environmental Microbiomics Research Center, School of Environmental Science and 
      Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology, Southern Marine Science and Engineering 
      Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou, 510006 China.
FAU - Liang, Zhiwei
AU  - Liang Z
AD  - Environmental Microbiomics Research Center, School of Environmental Science and 
      Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology, Southern Marine Science and Engineering 
      Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou, 510006 China.
FAU - Mai, Bixian
AU  - Mai B
AD  - State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of 
      Environmental Protection and Resources Utilization, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640 China.
FAU - Wang, Shanquan
AU  - Wang S
AD  - Environmental Microbiomics Research Center, School of Environmental Science and 
      Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology, Southern Marine Science and Engineering 
      Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou, 510006 China. 
      Electronic address: wangshanquan@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220607
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Butadienes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - CQ8AAO9MO1 (hexachlorobutadiene)
RN  - EC 1.12.7.2 (Hydrogenase)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/metabolism
MH  - Bacteria/metabolism
MH  - Biodegradation, Environmental
MH  - Butadienes
MH  - *Chloroflexi/genetics/metabolism
MH  - Dehalococcoides
MH  - Ecosystem
MH  - Humans
MH  - *Hydrogenase/metabolism
MH  - RNA, Ribosomal, 16S/genetics
OTO - NOTNLM
OT  - Co-metabolic dechlorination
OT  - Dehalococcoides
OT  - Electron transport chain
OT  - HCBD
OT  - Organohalide respiration
EDAT- 2022/06/17 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/16 18:19
PHST- 2022/02/18 00:00 [received]
PHST- 2022/05/06 00:00 [revised]
PHST- 2022/06/06 00:00 [accepted]
PHST- 2022/06/17 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
PHST- 2022/06/16 18:19 [entrez]
AID - S0043-1354(22)00678-9 [pii]
AID - 10.1016/j.watres.2022.118725 [doi]
PST - ppublish
SO  - Water Res. 2022 Jul 15;220:118725. doi: 10.1016/j.watres.2022.118725. Epub 2022 
      Jun 7.