PMID- 35710846
OWN - NLM
STAT- MEDLINE
DCOM- 20220715
LR  - 20220715
IS  - 1432-1955 (Electronic)
IS  - 0932-0113 (Linking)
VI  - 121
IP  - 8
DP  - 2022 Aug
TI  - Development of allele-specific PCR methodology (AS-PCR) to screening A. 
      lumbricoides and A. suum.
PG  - 2389-2397
LID - 10.1007/s00436-022-07572-x [doi]
AB  - Ascaris lumbricoides and Ascaris suum are described as helminths that infect 
      humans and pigs, respectively. It is estimated that infection by A. lumbricoides 
      affects about 447 million individuals living in tropical regions of developing 
      countries. However, there is an increasing number of cases of human ascariasis in 
      countries with no recent history of autochthonous infection by A. lumbricoides. 
      In these places, pigs have been incriminated as the main source of human 
      infection. Conventional parasitological diagnosis does not allow species-specific 
      identification, and the real epidemiological scenario of human and swine 
      ascariasis is still uncertain. Therefore, this work presents the application of a 
      species-specific molecular diagnosis, based on the allele-specific PCR 
      methodology (AS-PCR), using the Internal Transcript Space 1 (ITS-1) of the 
      ribosomal DNA, as a target for differentiating between the two species, using DNA 
      obtained from eggs. To validate the methodology, stool samples positive for 
      Ascaris spp, were obtained from 68 humans from seven Brazilian states and from 
      six pigs from the state of Minas Gerais. All samples obtained from humans were 
      genotyped as A. lumbricoides and all samples obtained from swine were genotyped 
      as A. suum. These results are in agreement with the literature, which 
      demonstrates that in most endemic regions, transmission cycles are separate. 
      Therefore, the execution of this work allowed the availability of a useful 
      methodology for the differential diagnosis of the species, which may contribute 
      to the characterization of the real epidemiological profile of human and swine 
      ascariasis, and to the implementation of future control strategies.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Dos Santos, Talita Rodrigues
AU  - Dos Santos TR
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - Furtado, Luis Fernando Viana
AU  - Furtado LFV
AD  - Universidade Do Estado de Minas Gerais, Unidade Passos, Avenida Juca Stockler, 
      1130, Minas Gerais, CEP 37900-106, Nossa Senhora das Gracas, Passos, Minas 
      Gerais, Brazil.
FAU - de Carvalho Araujo, Andreina
AU  - de Carvalho Araujo A
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - da Silva Medeiros, Celi
AU  - da Silva Medeiros C
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - Germano, Pedro Henrique Vieira
AU  - Germano PHV
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - de Oliveira, Valeria Nayara Gomes Mendes
AU  - de Oliveira VNGM
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - Rabelo, Elida Mara Leite
AU  - Rabelo EML
AD  - Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos, 6627, 
      Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil. 
      elidam.rabelo@gmail.com.
LA  - eng
GR  - APQ-01289-21/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/
GR  - APQ-02273-21/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/
PT  - Journal Article
DEP - 20220617
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
SB  - IM
MH  - Alleles
MH  - Animals
MH  - *Ascariasis/diagnosis/epidemiology/veterinary
MH  - Ascaris lumbricoides/genetics
MH  - *Ascaris suum/genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Swine
MH  - *Swine Diseases/diagnosis/epidemiology
OTO - NOTNLM
OT  - Ascaris lumbricoides
OT  - Ascaris suum
OT  - ITS-1
OT  - Species-specific PCR
EDAT- 2022/06/18 06:00
MHDA- 2022/07/16 06:00
CRDT- 2022/06/17 00:15
PHST- 2022/02/21 00:00 [received]
PHST- 2022/06/03 00:00 [accepted]
PHST- 2022/06/18 06:00 [pubmed]
PHST- 2022/07/16 06:00 [medline]
PHST- 2022/06/17 00:15 [entrez]
AID - 10.1007/s00436-022-07572-x [pii]
AID - 10.1007/s00436-022-07572-x [doi]
PST - ppublish
SO  - Parasitol Res. 2022 Aug;121(8):2389-2397. doi: 10.1007/s00436-022-07572-x. Epub 
      2022 Jun 17.