PMID- 35711792
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Protective Effects of Resveratrol and Apigenin Dietary Supplementation on Serum 
      Antioxidative Parameters and mRNAs Expression in the Small Intestines of 
      Diquat-Challenged Pullets.
PG  - 850769
LID - 10.3389/fvets.2022.850769 [doi]
LID - 850769
AB  - Poultry as a large-scale intensive farming is vulnerable to oxidative stress. 
      Resveratrol and apigenin are recognized to have many beneficial bioactive 
      functions. This study tested the hypothesis that dietary resveratrol and apigenin 
      supplementation alleviates oxidative stress in the small intestine of 
      diquat-challenged pullets. A total of 200 healthy pullets were randomly divided 
      into four treatment groups: control group fed with a basal diet (CON), diquat 
      group fed with a basal diet (DIQ), resveratrol group fed with a basal diet 
      containing 500 mg/kg resveratrol (RES), and an apigenin group fed with a basal 
      diet containing 500 mg/kg apigenin (API) and injected intraperitoneally with 
      either 1 ml of saline (CON) or 8 mg/kg body weight of diquat (DIQ, RES, and API) 
      to induce oxidative stress. The day of the injection was considered as day 0. The 
      results indicated that resveratrol and apigenin were able to decrease the 
      malondialdehyde (MDA) level and upregulate total antioxidant capacity (T-AOC), 
      superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) levels in serum 
      on day 1 and 10 after being diquat-challenged. In addition, resveratrol increased 
      mRNA expression of NQO1 (NAD(P)H dehydrogenase quinone 1) and HO-1 (heme 
      oxygenase-1) in ileum and jejunum on day 10, while apigenin upregulated nuclear 
      factor erythroid 2-related factor 2 (NRF2), NQO1, and HO-1 in ileum and jejunum 
      on day 10. Both resveratrol and apigenin increased the mRNA expression of 
      CLAUDIN-1 in ileum and jejunum on day 1 and that of ZO-1 (zonula occludens-1) in 
      ileum on day 10 post-diquat-injection. These findings indicate that dietary 
      supplementation with resveratrol and apigenin attenuates oxidative stress 
      involving NRF2 signaling pathways in diquat-challenged pullets to some extent. 
      These observations are valuable for the chicken industry and resveratrol and 
      apigenin applications in animal husbandry.
CI  - Copyright (c) 2022 Zhou, Tian, Liu, Tu, Xu, Gu, Zou and Lu.
FAU - Zhou, Ning
AU  - Zhou N
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Institute of Animal Science and Veterinary, Zhejiang 
      Academy of Agricultural Science, Hangzhou, China.
FAU - Tian, Yong
AU  - Tian Y
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Institute of Animal Science and Veterinary, Zhejiang 
      Academy of Agricultural Science, Hangzhou, China.
FAU - Liu, Wenchao
AU  - Liu W
AD  - Huzhou Lvchang Ecoagriculture Co., Ltd., Huzhou, China.
FAU - Tu, Bingjiang
AU  - Tu B
AD  - Animal Disease Prevention and Control Center, Huzhou Wuxing District Agricultural 
      and Rural Bureau, Huzhou, China.
FAU - Xu, Wenwu
AU  - Xu W
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Institute of Animal Science and Veterinary, Zhejiang 
      Academy of Agricultural Science, Hangzhou, China.
FAU - Gu, Tiantian
AU  - Gu T
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Institute of Animal Science and Veterinary, Zhejiang 
      Academy of Agricultural Science, Hangzhou, China.
FAU - Zou, Kang
AU  - Zou K
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Lu, Lizhi
AU  - Lu L
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-products, Institute of Animal Science and Veterinary, Zhejiang 
      Academy of Agricultural Science, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220530
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9196582
OTO - NOTNLM
OT  - apigenin
OT  - diquat
OT  - mRNA expression
OT  - oxidative stress
OT  - pullets
OT  - resveratrol
OT  - small intestine
COIS- WL was employed by Huzhou Lvchang Ecoagriculture Co., Ltd. The remaining authors 
      declare that the research was conducted in the absence of any commercial or 
      financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2022/06/18 06:00
MHDA- 2022/06/18 06:01
PMCR- 2022/01/01
CRDT- 2022/06/17 02:33
PHST- 2022/01/08 00:00 [received]
PHST- 2022/04/29 00:00 [accepted]
PHST- 2022/06/17 02:33 [entrez]
PHST- 2022/06/18 06:00 [pubmed]
PHST- 2022/06/18 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.850769 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 May 30;9:850769. doi: 10.3389/fvets.2022.850769. eCollection 
      2022.