PMID- 35713800 OWN - NLM STAT- MEDLINE DCOM- 20220725 LR - 20220725 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 49 IP - 8 DP - 2022 Aug TI - NTRK2 gene fusions are uncommon in pilocytic astrocytoma. PG - 7567-7573 LID - 10.1007/s11033-022-07567-y [doi] AB - BACKGROUND: Pilocytic astrocytoma is the most frequent pediatric glioma. Despite its overall good prognosis, complete surgical resection is sometimes unfeasible, especially for patients with deep-seated tumors. For these patients, the identification of targetable genetic alterations such as NTRK fusions, raised as a new hope for therapy. The presence of gene fusions involving NTRK2 has been rarely reported in pilocytic astrocytoma. The aim of the present study was to investigate the frequency of NTRK2 alterations in a series of Brazilian pilocytic astrocytomas. METHODS: Sixty-nine pilocytic astrocytomas, previously characterized for BRAF and FGFR1 alterations were evaluated. The analysis of NTRK2 alterations was performed using a dual color break apart fluorescence in situ hybridization (FISH) assay. RESULTS: NTRK2 fusions were successfully evaluated by FISH in 62 of the 69 cases. Neither evidence of NTRK2 gene rearrangements nor NTRK2 copy number alterations were found. CONCLUSIONS: NTRK2 alterations are uncommon genetic events in pilocytic astrocytomas, regardless of patients' clinicopathological and molecular features. CI - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Moreno, Daniel Antunes AU - Moreno DA AD - Molecular Oncology Research Center, Barretos Cancer Hospital, 1331 Antenor Duarte Vilela St, 14784-400, Barretos, SP, Brazil. FAU - Becker, Aline Paixao AU - Becker AP AD - James Cancer Hospital and Solove Research Institute Columbus, Ohio, USA. FAU - Scapulatempo-Neto, Cristovam AU - Scapulatempo-Neto C AD - Department of Pathology, Barretos Cancer Hospital, Barretos, Sao Paulo, Brazil. FAU - Menezes, Weder AU - Menezes W AD - Molecular Oncology Research Center, Barretos Cancer Hospital, 1331 Antenor Duarte Vilela St, 14784-400, Barretos, SP, Brazil. FAU - Sheren, Jamie AU - Sheren J AD - University of Colorado School of Medicine, Aurora, CO, USA. AD - Department of Pathology, Colorado Molecular Correlates Laboratory (CMOCO), Molecular Pathology Shared Resource, Cytogenetics - Anschutz Medical Campus - RC1-South, Rm L18-8401A 12801 E 17th Ave, 80045, Aurora, CO, USA. FAU - Walter, Aline M AU - Walter AM AD - University of Colorado School of Medicine, Aurora, CO, USA. AD - Department of Pathology, Colorado Molecular Correlates Laboratory (CMOCO), Molecular Pathology Shared Resource, Cytogenetics - Anschutz Medical Campus - RC1-South, Rm L18-8401A 12801 E 17th Ave, 80045, Aurora, CO, USA. FAU - Clara, Carlos AU - Clara C AD - Department of Neurosurgery, Barretos Cancer Hospital, 1331 Antenor Duarte Vilela St, 14784-400, Barretos, SP, Brazil. FAU - Machado, Helio R AU - Machado HR AD - Department of Surgery, Ribeirao Preto, Brazil. FAU - Oliveira, Ricardo S AU - Oliveira RS AD - Department of Surgery, Ribeirao Preto, Brazil. FAU - Neder, Luciano AU - Neder L AD - Department of Pathology of Ribeirao Preto Medical School, University of Sao Paulo (FMRP-USP) - Campus of University of Sao Paulo, 14049-900, Ribeirao Preto, SP, Brazil. FAU - Varella-Garcia, Marileila AU - Varella-Garcia M AD - University of Colorado School of Medicine, Aurora, CO, USA. AD - Department of Pathology, Colorado Molecular Correlates Laboratory (CMOCO), Molecular Pathology Shared Resource, Cytogenetics - Anschutz Medical Campus - RC1-South, Rm L18-8401A 12801 E 17th Ave, 80045, Aurora, CO, USA. FAU - Reis, Rui Manuel AU - Reis RM AUID- ORCID: 0000-0002-9639-7940 AD - Molecular Oncology Research Center, Barretos Cancer Hospital, 1331 Antenor Duarte Vilela St, 14784-400, Barretos, SP, Brazil. ruireis.hcb@gmail.com. AD - Life and Health Sciences Research Institute (ICVS), Medical School, University of Minho, Braga, Portugal. ruireis.hcb@gmail.com. AD - ICVS/3B's - PT Government Associate Laboratory, Braga, Guimaraes, Portugal. ruireis.hcb@gmail.com. LA - eng GR - CNPq- 475358/2011-2/National Council for Scientific and Technological Development/ GR - FAPESP -2012/19590-0/Sao Paulo Foundation for Research Support/ GR - NIH-P30CA046934 (CCSG Molecular Pathology/Cytogenetics)/NIH-P30CA046934 (CCSG Molecular Pathology/Cytogenetics)/ GR - Coordination for the Improvement of Level Personnel (CAPES, Brazil)/Coordination for the Improvement of Level Personnel (CAPES, Brazil)/ PT - Journal Article DEP - 20220617 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf) SB - IM MH - *Astrocytoma/genetics MH - *Brain Neoplasms/genetics/pathology MH - Gene Fusion MH - *Glioma/genetics MH - Humans MH - In Situ Hybridization, Fluorescence MH - Proto-Oncogene Proteins B-raf/genetics OTO - NOTNLM OT - FISH. OT - Gene fusions OT - NTRK2 OT - Pilocytic astrocytoma OT - Tyrosine kinases EDAT- 2022/06/18 06:00 MHDA- 2022/07/26 06:00 CRDT- 2022/06/17 11:20 PHST- 2022/02/19 00:00 [received] PHST- 2022/05/04 00:00 [accepted] PHST- 2022/04/23 00:00 [revised] PHST- 2022/06/18 06:00 [pubmed] PHST- 2022/07/26 06:00 [medline] PHST- 2022/06/17 11:20 [entrez] AID - 10.1007/s11033-022-07567-y [pii] AID - 10.1007/s11033-022-07567-y [doi] PST - ppublish SO - Mol Biol Rep. 2022 Aug;49(8):7567-7573. doi: 10.1007/s11033-022-07567-y. Epub 2022 Jun 17.