PMID- 35714457
OWN - NLM
STAT- MEDLINE
DCOM- 20220721
LR  - 20220906
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 573
DP  - 2022 Aug
TI  - Molecular characterization of emerging variants of PRRSV in the United States: 
      new features of the -2/-1 programmed ribosomal frameshifting signal in the nsp2 
      region.
PG  - 39-49
LID - S0042-6822(22)00090-3 [pii]
LID - 10.1016/j.virol.2022.06.004 [doi]
AB  - In this study, we characterized an emerging porcine reproductive and respiratory 
      syndrome virus (PRRSV) isolate UIL21-0712, which is a lineage 1C variant with 
      ORF5 restriction fragment length polymorphism (RFLP) cutting pattern of 1-4-4. 
      The UIL21-0712 genome sequence has 85.3% nucleotide identity with the prototypic 
      PRRSV-2 strain VR2332. The nsp2 region is the most variable, and the -2/-1 
      programmed ribosome frameshifting (PRF) signal therein is distinct from 
      historical PRRSV strains. Analysis of PRRSV sequences in GenBank revealed that 
      the majority of the emerging PRRSV variants contain substitutions that disrupt 
      the -1 PRF stop codon to generate a nsp2N protein with a C-terminal extension. 
      Two of the -1 PRF stop codon variant patterns were identified to be predominantly 
      circulating in the field. They demonstrated higher growth kinetics than the other 
      variants, suggesting that the most dominant -1 PRF stop codon variant patterns 
      may provide enhanced growth fitness for the virus.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Yan, Xingyu
AU  - Yan X
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, Urbana-Champaign, Urbana, IL, USA; Department of Diagnostic Medicine 
      and Pathobiology, College of Veterinary Medicine, Kansas State University, 
      Manhattan, KS, USA.
FAU - Shang, Pengcheng
AU  - Shang P
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS, USA.
FAU - Yim-Im, Wannarat
AU  - Yim-Im W
AD  - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 
      Ames, IA, USA.
FAU - Sun, Yankuo
AU  - Sun Y
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, Urbana-Champaign, Urbana, IL, USA.
FAU - Zhang, Jianqiang
AU  - Zhang J
AD  - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 
      Ames, IA, USA.
FAU - Firth, Andrew E
AU  - Firth AE
AD  - Department of Pathology, University of Cambridge, Cambridge, UK.
FAU - Lowe, James F
AU  - Lowe JF
AD  - Department of Veterinary Clinical Medicine, College of Veterinary Medicine, 
      University of Illinois, Urbana-Champaign, Urbana, IL, USA.
FAU - Fang, Ying
AU  - Fang Y
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, Urbana-Champaign, Urbana, IL, USA; Department of Diagnostic Medicine 
      and Pathobiology, College of Veterinary Medicine, Kansas State University, 
      Manhattan, KS, USA. Electronic address: yingf@illinois.edu.
LA  - eng
GR  - 106207/WT_/Wellcome Trust/United Kingdom
GR  - 220814/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20220605
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Codon, Terminator)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Codon, Terminator
MH  - Frameshifting, Ribosomal
MH  - Genetic Variation
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - United States
EDAT- 2022/06/18 06:00
MHDA- 2022/07/22 06:00
CRDT- 2022/06/17 18:16
PHST- 2022/04/14 00:00 [received]
PHST- 2022/05/31 00:00 [revised]
PHST- 2022/06/01 00:00 [accepted]
PHST- 2022/06/18 06:00 [pubmed]
PHST- 2022/07/22 06:00 [medline]
PHST- 2022/06/17 18:16 [entrez]
AID - S0042-6822(22)00090-3 [pii]
AID - 10.1016/j.virol.2022.06.004 [doi]
PST - ppublish
SO  - Virology. 2022 Aug;573:39-49. doi: 10.1016/j.virol.2022.06.004. Epub 2022 Jun 5.