PMID- 35715052
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220621
IS  - 1095-9939 (Electronic)
IS  - 0048-3575 (Linking)
VI  - 184
DP  - 2022 Jun
TI  - Imazalil and its metabolite imazalil-M caused developmental toxicity in zebrafish 
      (Danio rerio) embryos via cell apoptosis mediated by metabolic disorders.
PG  - 105113
LID - S0048-3575(22)00080-3 [pii]
LID - 10.1016/j.pestbp.2022.105113 [doi]
AB  - Imazalil (IMZ) is a highly effective fungicide employed in crop production. It 
      has been consistently detected in aquatic environments. The main environmental 
      metabolite of IMZ is imazalil-M (IMZ-M). Limited studies have focused on the 
      toxicity of IMZ and IMZ-M in aquatic organisms. This study systematically 
      evaluated the developmental toxicity of IMZ and IMZ-M on zebrafish (Danio rerio) 
      embryos and explored the potential mechanisms involved. The results showed that 
      IMZ and IMZ-M caused developmental toxicity, characterized by decreased heart 
      rate, hatching inhibition, and pericardial cyst in zebrafish embryos. 
      Subsequently, acridine orange (AO) staining revealed cell apoptosis in the area 
      around the heart regions of zebrafish larvae. Besides, the expression levels of 
      apoptosis-related genes also varied significantly. Furthermore, (1)H NMR-based 
      metabolomics analysis showed that IMZ and IMZ-M exposure could induce metabolic 
      profiles disorder in zebrafish larvae. Importantly, zebrafish exposure to IMZ and 
      IMZ-M significantly affected the metabolism of branched - chain amino acids, 
      energy, and ketone bodies, which are related to cell apoptosis. Overall, the 
      toxicity of IMZ and IMZ-M in zebrafish embryos and larvae was characterized, 
      suggesting a theoretical basis for the potential environmental risks of IMZ and 
      its metabolite IMZ-M on non-target organisms.
CI  - Copyright (c) 2022. Published by Elsevier Inc.
FAU - Huang, Shiran
AU  - Huang S
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China.
FAU - Huang, Ming
AU  - Huang M
AD  - College of Science, China Agricultural University, Beijing 100193, China.
FAU - Tian, Sinuo
AU  - Tian S
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China.
FAU - Meng, Zhiyuan
AU  - Meng Z
AD  - School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 
      Jiangsu 225009, China.
FAU - Yan, Sen
AU  - Yan S
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China.
FAU - Teng, Miaomiao
AU  - Teng M
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Sciences, Beijing 100012, China.
FAU - Zhou, Zhiqiang
AU  - Zhou Z
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China.
FAU - Diao, Jinling
AU  - Diao J
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China.
FAU - Zhu, Wentao
AU  - Zhu W
AD  - Innovation Center of Pesticide Research, Department of Applied Chemistry, College 
      of Science, China Agricultural University, Beijing 100193, China. Electronic 
      address: wentaozhu@cau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220502
PL  - United States
TA  - Pestic Biochem Physiol
JT  - Pesticide biochemistry and physiology
JID - 1301573
RN  - 0 (Imidazoles)
RN  - 0 (Water Pollutants, Chemical)
RN  - 6K0NOF3XQ6 (enilconazole)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Embryo, Nonmammalian
MH  - Imidazoles/toxicity
MH  - Larva
MH  - *Metabolic Diseases/metabolism
MH  - *Water Pollutants, Chemical/toxicity
MH  - Zebrafish/genetics
OTO - NOTNLM
OT  - Cell apoptosis
OT  - Developmental toxicity
OT  - Metabolomics
OT  - PCR array
OT  - Zebrafish embryos
EDAT- 2022/06/18 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/17 21:05
PHST- 2022/03/06 00:00 [received]
PHST- 2022/04/15 00:00 [revised]
PHST- 2022/04/28 00:00 [accepted]
PHST- 2022/06/17 21:05 [entrez]
PHST- 2022/06/18 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
AID - S0048-3575(22)00080-3 [pii]
AID - 10.1016/j.pestbp.2022.105113 [doi]
PST - ppublish
SO  - Pestic Biochem Physiol. 2022 Jun;184:105113. doi: 10.1016/j.pestbp.2022.105113. 
      Epub 2022 May 2.