PMID- 35715914
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220621
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 18
IP  - 3
DP  - 2022 Mar 1
TI  - Photothermolysis Mediated by Gold Nanorods Conjugated with Epidermal Growth 
      Factor Receptor (EGFR) Monoclonal Antibody Induces Apoptosis via the 
      Mitochondrial Apoptosis Pathway in Laryngeal Squamous Cell Cancer.
PG  - 754-762
LID - 10.1166/jbn.2022.3272 [doi]
AB  - Gold nanorods (AuNRs) have unique optical properties and biological affinity and 
      can be used to treat tumors when conjugated with other protein molecules. Our 
      previous studies have shown that EGFR monoclonal antibody (EGFRmAb)-modified 
      AuNRs exert strong antitumor activity in vitro by inducing apoptosis. In this 
      study, we tested the effects of EGFRmAb-modified AuNRs on laryngeal squamous cell 
      cancer (LSCC) in vitro and in vivo. The in vitro results showed that 
      EGFRmAb-modified AuNRs inhibited NP-69, BEAS-2B and Hep-2 cell growth and induced 
      mitochondria-dependent apoptosis. The mitochondrial membrane potential was 
      reduced, leading to the release of cytochrome C (Cyt C) and consequent activation 
      of the intrinsic mitochondrial apoptosis pathway. Moreover, we observed that the 
      occurrence of mitochondrial apoptosis is related to the destruction of the 
      lysosome-mitochondria axis. To verify the effects in vivo, we also established a 
      laryngeal tumor model in nude mice by subcutaneous transplantation. In model mice 
      treated with EGFRmAb-modified AuNRs and irradiated with an NIR laser, tumor cell 
      apoptosis and tumor growth were inhibited. These results suggest that 
      EGFRmAb-modified AuNRs induced apoptosis through the intrinsic mitochondrial 
      apoptotic pathway and are a potential candidate for cancer therapy.
FAU - Zhang, Shi-Wen
AU  - Zhang SW
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Wang, Hao
AU  - Wang H
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Qiu, You-Yu
AU  - Qiu YY
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Huang, Ren-Chao
AU  - Huang RC
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Dong, Zi-Chen
AU  - Dong ZC
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Zhang, Lu
AU  - Zhang L
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Zhao, Liu-Fang
AU  - Zhao LF
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Xu, Hong-Yang
AU  - Xu HY
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
FAU - Sun, Wei-Di
AU  - Sun WD
AD  - First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third 
      Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Antibodies, Monoclonal)
RN  - 7440-57-5 (Gold)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Epithelial Cells
MH  - ErbB Receptors/metabolism
MH  - Gold/pharmacology
MH  - *Head and Neck Neoplasms/metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Mitochondria/metabolism
MH  - *Nanotubes
MH  - Squamous Cell Carcinoma of Head and Neck/drug therapy/metabolism
EDAT- 2022/06/19 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/18 00:42
PHST- 2022/06/18 00:42 [entrez]
PHST- 2022/06/19 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
AID - 10.1166/jbn.2022.3272 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2022 Mar 1;18(3):754-762. doi: 10.1166/jbn.2022.3272.