PMID- 35716001
OWN - NLM
STAT- MEDLINE
DCOM- 20220810
LR  - 20220815
IS  - 1447-0756 (Electronic)
IS  - 1341-8076 (Linking)
VI  - 48
IP  - 8
DP  - 2022 Aug
TI  - MiR-195-5p facilitates the proliferation, migration, and invasion of human 
      trophoblast cells by targeting FGF2.
PG  - 2122-2133
LID - 10.1111/jog.15298 [doi]
AB  - BACKGROUND: Preeclampsia (PE), the most significant adverse exposure to 
      cardiovascular risk during pregnancy, is one of the three major factors 
      contributing to maternal and fetal mortality and the leading cause of preterm 
      birth. Recently, various miRNAs have been reported to participate in PE 
      occurrence and development. Nevertheless, the regulatory impact of miR-195-5p in 
      PE is still indistinct. METHODS: Quantitative realtime-PCR (qRT-PCR), western 
      blot, and fluorescence in situ hybridization (FISH) assay were performed to 
      examine miR-195-5p and FGF2 expressions in PE serum samples or HTR-8/SVneo and 
      TEV-1 cells. CCK8, flow cytometry, wound scratch, and transwell assays were 
      conducted to determine cell viability, cycle, apoptosis, migration, and invasion. 
      Dual-luciferase reporter assay unveiled the relationship between miR-195-5p and 
      FGF2. Migration-related and invasion-related protein expressions were measured by 
      western blot assay. RESULTS: miR-195-5p was prominently downregulated while FGF2 
      was increased in serum samples from PE patients and hypoxia-treated human 
      trophoblast cells. FGF2 was predicted as a downstream target of miR-195-5p and 
      targeted association was verified by dual-luciferase reporter assay. Functional 
      experiments elaborated that miR-195-5p could facilitate trophoblast cell 
      proliferation and metastasis but hinder cell cycle and apoptosis. Inversely, 
      overexpressing of FGF2 could reverse the effects of miR-195-5p on trophoblast 
      cell growth. DISCUSSION: miR-195-5p was decreased in PE serum samples and cell 
      lines, serving as a potential biomarker in protecting PE exacerbation by 
      targeting FGF2.
CI  - (c) 2022 Japan Society of Obstetrics and Gynecology.
FAU - Zhou, Dachun
AU  - Zhou D
AD  - Department of Gynaecology and Obstetrics, Nantong Maternal and Child Health Care 
      Hospital, Nantong, Jiangsu Province, China.
FAU - Xu, Xiaoying
AU  - Xu X
AD  - Department of Gynaecology and Obstetrics, Haian People's Hospital, Haian, Jiangsu 
      Province, China.
FAU - Liu, Yuanlin
AU  - Liu Y
AD  - Department of Gynaecology and Obstetrics, Shanghai First Maternity and Infant 
      Hospital, Shanghai, China.
AD  - School of Medicine, Tongji University, Shanghai, China.
FAU - Liu, Haiyun
AU  - Liu H
AD  - Department of Gynaecology and Obstetrics, Nantong Maternal and Child Health Care 
      Hospital, Nantong, Jiangsu Province, China.
FAU - Cheng, Xiaoyan
AU  - Cheng X
AD  - Department of Gynaecology and Obstetrics, Nantong Maternal and Child Health Care 
      Hospital, Nantong, Jiangsu Province, China.
FAU - Gu, Yannan
AU  - Gu Y
AD  - Department of Gynaecology and Obstetrics, Nantong Maternal and Child Health Care 
      Hospital, Nantong, Jiangsu Province, China.
FAU - Xu, Yuanyuan
AU  - Xu Y
AD  - Department of Ultrasound, Nantong Maternal and Child Health Care Hospital, 
      Nantong, Jiangsu Province, China.
FAU - Zhu, Lingling
AU  - Zhu L
AUID- ORCID: 0000-0003-0947-3819
AD  - Department of Gynaecology and Obstetrics, Nantong Maternal and Child Health Care 
      Hospital, Nantong, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20220618
PL  - Australia
TA  - J Obstet Gynaecol Res
JT  - The journal of obstetrics and gynaecology research
JID - 9612761
RN  - 0 (MIRN195 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Fibroblast Growth Factor 2/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - *MicroRNAs/metabolism
MH  - *Pre-Eclampsia/genetics/metabolism
MH  - Pregnancy
MH  - *Premature Birth/metabolism
MH  - Trophoblasts/metabolism
OTO - NOTNLM
OT  - FGF2
OT  - miR-195-5p
OT  - preeclampsia
OT  - trophoblast cells
EDAT- 2022/06/19 06:00
MHDA- 2022/08/11 06:00
CRDT- 2022/06/18 03:42
PHST- 2022/04/12 00:00 [revised]
PHST- 2021/11/04 00:00 [received]
PHST- 2022/05/10 00:00 [accepted]
PHST- 2022/06/19 06:00 [pubmed]
PHST- 2022/08/11 06:00 [medline]
PHST- 2022/06/18 03:42 [entrez]
AID - 10.1111/jog.15298 [doi]
PST - ppublish
SO  - J Obstet Gynaecol Res. 2022 Aug;48(8):2122-2133. doi: 10.1111/jog.15298. Epub 
      2022 Jun 18.