PMID- 35716385
OWN - NLM
STAT- MEDLINE
DCOM- 20220805
LR  - 20220810
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 36
IP  - 17
DP  - 2022 Sep 15
TI  - Detoxification mechanism of vinegar-processed Kansui revealed by systematic 
      phytochemical analysis using ultrahigh-performance liquid chromatography diode 
      array detection tandem mass spectrometry, ultrahigh-performance liquid 
      chromatography high-resolution mass spectrometry and in silico drug target 
      identification.
PG  - e9332
LID - 10.1002/rcm.9332 [doi]
AB  - RATIONALE: The dried roots of Euphorbia kansui L., known as Kansui, are used to 
      treat ascites and edema in traditional Chinese medicine. However, the toxicity of 
      this herb has seriously restricted its clinical application. A unique 
      vinegar-processing method has been used to reduce its toxicity since the time of 
      ancient China. However, the detoxification mechanism underlying such vinegar 
      processing has not been fully revealed. To find the answer, the process-induced 
      changes in components should be carefully investigated. METHODS: We performed a 
      systematic analysis of chemical components in raw and vinegar-processed Kansui 
      using ultrahigh-performance liquid chromatography (UHPLC) diode array detection 
      tandem mass spectrometry and UHPLC high-resolution mass spectrometry. Thirty-one 
      chemical components in raw and vinegar-processed Kansui were found, the chemical 
      structures of 28 components among them were proposed and the process-induced 
      changes in components were then investigated. RESULTS: A comprehensive conclusion 
      about the process-induced chemical change was drawn. It was found that 
      jatrophane-type diterpenoids decreased markedly after vinegar processing, while 
      ingenane-type diterpenoids were retained during vinegar processing. In silico 
      drug target identification gave hints that jatrophane-type diterpenoids, which 
      decreased markedly during vinegar processing, may have more intense toxicity 
      involving cholinesterase and mitogen-activated protein kinases, while 
      ingenane-type diterpenoids, which were retained during vinegar processing, may 
      have a more intense therapeutic effect involving carbonic anhydrase. CONCLUSIONS: 
      The possible detoxification mechanism of vinegar-processed Kansui is presented. 
      The research has significance for the therapeutic/toxic chemical basis of Kansui. 
      Also, it has significance for drug discovery from terpenoids within the herb.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Liu, Lian
AU  - Liu L
AD  - Pharmaceutical Research Institute, Wuhan Institute of Technology, Wuhan, China.
FAU - Li, Jiangchun
AU  - Li J
AD  - Hubei Key Laboratory of Forensic Science, Hubei University of Police, Wuhan, 
      China.
FAU - Lv, Jizhong
AU  - Lv J
AD  - Physical and Chemical Investigation Laboratory, Hubei Province Public Security 
      Department, Wuhan, Hubei, China.
FAU - Jiang, Haipeng
AU  - Jiang H
AUID- ORCID: 0000-0001-8600-6566
AD  - Pharmaceutical Research Institute, Wuhan Institute of Technology, Wuhan, China.
FAU - Chen, Fen-Er
AU  - Chen FE
AD  - Pharmaceutical Research Institute, Wuhan Institute of Technology, Wuhan, China.
AD  - Engineering Center of Catalysis and Synthesis for Chiral Molecules, Department of 
      Chemistry, Fudan University, Shanghai, China.
LA  - eng
GR  - KFKT2019008/Open Grant of Hubei Key Laboratory of the Forensic Science/
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Diterpenes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - Q40Q9N063P (Acetic Acid)
SB  - IM
MH  - *Acetic Acid/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - *Diterpenes/analysis/chemistry/pharmacology
MH  - *Drugs, Chinese Herbal/chemistry
MH  - *Euphorbia/chemistry
MH  - Phytochemicals/analysis
MH  - Plant Extracts/toxicity
MH  - *Plant Roots/adverse effects/chemistry
MH  - Tandem Mass Spectrometry/methods
EDAT- 2022/06/19 06:00
MHDA- 2022/08/06 06:00
CRDT- 2022/06/18 14:22
PHST- 2022/06/08 00:00 [revised]
PHST- 2022/05/07 00:00 [received]
PHST- 2022/06/12 00:00 [accepted]
PHST- 2022/06/19 06:00 [pubmed]
PHST- 2022/08/06 06:00 [medline]
PHST- 2022/06/18 14:22 [entrez]
AID - 10.1002/rcm.9332 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2022 Sep 15;36(17):e9332. doi: 10.1002/rcm.9332.