PMID- 35718231
OWN - NLM
STAT- MEDLINE
DCOM- 20220707
LR  - 20220725
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 431
DP  - 2022 Aug 5
TI  - Effects of thymoquinone on scopolamine-induced spatial and echoic memory changes 
      through regulation of lipid peroxidation and cholinergic impairment.
PG  - 113972
LID - S0166-4328(22)00240-6 [pii]
LID - 10.1016/j.bbr.2022.113972 [doi]
AB  - Thymoquinone (TMQ), one of the main components active of Nigella sativa, shows 
      very useful biomedical properties. Evidence suggests that cholinergic dysfunction 
      and oxidative stress play role in the pathogenesis of neurodegenerative disorders 
      such as Alzheimer's disease (AD). In the present study, we investigated the 
      anti-amnestic effect of TMQ in scopolamine-induced animal model of AD. Wistar 
      rats were randomly divided into four groups; Sham(SH), TMQ-treated(TMQ), 
      scopolamine-treated(SCO) and scopolamine+TMQ-treated(SCO_TMQ) groups. TMQ 
      (20 mg/kg) prepared in corn oil was administered intraperitoneally (i.p.) 1-h 
      before experiments. Scopolamine (1 mg/kg) dissolved in 0.9% physiological saline 
      was administered intraperitoneally (i.p.). We recorded mismatch negativity (MMN) 
      response as an electrophysiological correlate of echoic memory. Object location 
      memory (OLM) and Y-maze alternation tests were carried out to assess spatial 
      memory. Then, the brain homogenates content of 
      thiobarbituric-acid-reactive-substances (TBARS), 4-Hydroxy-2-nonenal (4-HNE) and 
      acetylcholine (ACh)/acetylcholine (AChE) activity were biochemically determined. 
      In the scopolamine-treated rats, TMQ was found to significantly improve the 
      discrimination and spontaneous alteration levels in the OLM and Y-maze tests, 
      respectively. Furthermore, TMQ significantly mitigated the scopolamine-induced 
      attenuation of MMN and related theta responses. Moreover, scopolamine treatment 
      increased TBARS/4-HNE level and decreased ACh level in the brain, and TMQ was 
      able to significantly prevent these effects. AChE activity was increased in the 
      SCO group; this effect was significantly attenuated by TMQ. TMQ diminished the 
      lipid peroxidation and cholinergic dysfunction in the scopolamine-induced AD rat 
      model which all reflected in improving the MMN/theta response and spatial memory. 
      This may implement TMQ as an adjuvant therapeutic strategy in ameliorating AD.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Kantar, Deniz
AU  - Kantar D
AD  - Department of Biophysics, Faculty of Medicine, Akdeniz University, Antalya, 
      Turkey. Electronic address: dkantar@akdeniz.edu.tr.
FAU - Acun, Alev Duygu
AU  - Acun AD
AD  - Department of Biophysics, Faculty of Medicine, Akdeniz University, Antalya, 
      Turkey.
FAU - Danisman, Betul
AU  - Danisman B
AD  - Department of Biophysics, Faculty of Medicine, Ataturk University, Erzurum, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20220616
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Benzoquinones)
RN  - 0 (Cholinergic Agents)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - DL48G20X8X (Scopolamine)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - N9YNS0M02X (Acetylcholine)
RN  - O60IE26NUF (thymoquinone)
SB  - IM
MH  - *Acetylcholine
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Benzoquinones
MH  - Cholinergic Agents/pharmacology
MH  - Hippocampus/metabolism
MH  - Lipid Peroxidation
MH  - Maze Learning
MH  - Memory Disorders/chemically induced/drug therapy
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Wistar
MH  - *Scopolamine/pharmacology
MH  - Thiobarbituric Acid Reactive Substances
OTO - NOTNLM
OT  - Cholinergic system
OT  - Lipid peroxidation
OT  - Memory
OT  - Mismatch negativity
OT  - Scopolamine
OT  - Thymoquinone
EDAT- 2022/06/20 06:00
MHDA- 2022/07/08 06:00
CRDT- 2022/06/19 19:34
PHST- 2022/02/09 00:00 [received]
PHST- 2022/05/23 00:00 [revised]
PHST- 2022/06/14 00:00 [accepted]
PHST- 2022/06/20 06:00 [pubmed]
PHST- 2022/07/08 06:00 [medline]
PHST- 2022/06/19 19:34 [entrez]
AID - S0166-4328(22)00240-6 [pii]
AID - 10.1016/j.bbr.2022.113972 [doi]
PST - ppublish
SO  - Behav Brain Res. 2022 Aug 5;431:113972. doi: 10.1016/j.bbr.2022.113972. Epub 2022 
      Jun 16.