PMID- 35718914
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220621
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 51
IP  - 3
DP  - 2022 May
TI  - [Determination of 4 chlorpromazine and promethazine and their metabolites in 
      swine tissues by liquid chromatography-tandem mass spectrometry with isotope 
      internal standard dilution technique].
PG  - 476-482
LID - 10.19813/j.cnki.weishengyanjiu.2022.03.022 [doi]
AB  - OBJECTIVE: To establish a rapid, accurate and sensitive method by liquid 
      chromatography-tandem mass spectrometry with isotope internal standard dilution 
      technique for the determination of chlorpromazine and promethazine and their 
      metabolites in swine tissues. METHODS: The swine tissues sample was extracted 
      with acetonitrile and purified on MCX cartridge. The liquid chromatography 
      separation was performed on an ACQUITY UFLC(R) HSS T3(100 mmx2.1 mm, 1.8 mum) with a 
      linear gradient elution program of 0.1%(V/V) fomic acid-acetonitrile and 
      0.1%(V/V) formic acid-water solution as the mobile phase. The analytes were 
      analyzed using ESI operating in the positive multiple reaction monitoring(MRM) 
      mode. RESULTS: The limits of quantitation(LOQs) and limits of detection(LODs) for 
      the target objects were 0.12-0.51 mug/kg and 0.04-0.17 mug/kg, respectively. The 
      calibration curves were linear in range of 0.1-20.0 mug/L for chlorpromazine and 
      promethazine, and 0.5-100.0 mug/L for their metabolites(chlorpromazine sulfoxide 
      and isopropyl sulfoxide). The recoveries were between 90.8%-106.0%, and the 
      relative standard deviations(RSDs) were between 1.9%-6.2%(n=6). CONCLUSION: The 
      method is highly sensitive and accurate, and is suitable for the analysis of 
      chlorpromazine and promethazine and their metabolites(chlorpromazine sulfoxide 
      and isopropyl sulfoxide) in swine tissues.
FAU - Chen, Xiaohong
AU  - Chen X
AD  - Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang 
      Province, Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal 
      Center for Disease Control and Prevention, Ningbo 315010, China.
FAU - Zhou, Jian
AU  - Zhou J
AD  - Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang 
      Province, Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal 
      Center for Disease Control and Prevention, Ningbo 315010, China.
FAU - Jin, Micong
AU  - Jin M
AD  - Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang 
      Province, Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal 
      Center for Disease Control and Prevention, Ningbo 315010, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Acetonitriles)
RN  - 0 (Isotopes)
RN  - 0 (Sulfoxides)
RN  - FF28EJQ494 (Promethazine)
RN  - U42B7VYA4P (Chlorpromazine)
SB  - IM
MH  - Acetonitriles
MH  - Animals
MH  - Chlorpromazine
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Indicator Dilution Techniques
MH  - Isotopes
MH  - *Promethazine
MH  - Solid Phase Extraction
MH  - Sulfoxides
MH  - Swine
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - chlorpromazine
OT  - chlorpromazine sulfoxide
OT  - isopropyl sulfoxide
OT  - liquid chromatography-tandem mass spectrometry(LC-MS/MS)
OT  - promethazine
OT  - swine tissues
EDAT- 2022/06/21 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/20 00:12
PHST- 2022/06/20 00:12 [entrez]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2022.03.022 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2022 May;51(3):476-482. doi: 
      10.19813/j.cnki.weishengyanjiu.2022.03.022.