PMID- 35718998
OWN - NLM
STAT- MEDLINE
DCOM- 20220715
LR  - 20220716
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 36
IP  - 7
DP  - 2022 Jul
TI  - Identification of potential gene markers in gestational diabetes mellitus.
PG  - e24515
LID - 10.1002/jcla.24515 [doi]
LID - e24515
AB  - This study aims to investigate underlying mechanisms of gestational diabetes 
      mellitus (GDM). In this work, the GSE70493 dataset from GDM and control samples 
      was acquired from Gene Expression Omnibus (GEO) database. Afterward, 
      differentially expressed genes (DEGs) were screened between GDM and control 
      samples. Subsequently, functional enrichment analysis and protein-protein 
      interaction (PPI) network analysis of these DEGs were carried out. Furthermore, 
      significant sub-modules were identified, and the functional analysis was also 
      performed. Finally, we undertook a quantitative real-time polymerase chain 
      reaction (qRT-PCR) with the purpose of confirming several key genes in GDM 
      development. There were totally 528 up-regulated and 684 down-regulated DEGs 
      between GDM and healthy samples. The functional analyses suggested that the above 
      genes were dramatically enriched in type 1 diabetes mellitus (T1DM) process and 
      immune-related pathways. Moreover, PPI analysis revealed that several members of 
      human leukocyte antigen (HLA) superfamily, including down-regulated HLA-DQA1, 
      HLA-DRB1, HLA-DPA1, and HLA-DQB1 served as hub genes. In addition, six 
      significant sub-clusters were extracted and functional analysis suggested that 
      these four genes in sub-module 1 were also associated with immune and 
      T1DM-related pathways. Finally, they were also confirmed by qRT-PCR array. 
      Besides, the four members of HLA superfamily might be implicated with molecular 
      mechanisms of GDM, contributing to a deeper understanding of GDM development.
CI  - (c) 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Tang, Weichun
AU  - Tang W
AD  - The Department of Obstetrics and Gynecology, The Affiliated Hospital 2 of Nantong 
      University, Nantong, China.
FAU - Wang, Xiaoyu
AU  - Wang X
AD  - The Department of Obstetrics and Gynecology, The Affiliated Hospital 2 of Nantong 
      University, Nantong, China.
FAU - Chen, Liping
AU  - Chen L
AUID- ORCID: 0000-0001-8288-4062
AD  - The Department of Obstetrics and Gynecology, The Affiliated Hospital 2 of Nantong 
      University, Nantong, China.
FAU - Lu, Yiling
AU  - Lu Y
AD  - The Department of Obstetrics and Gynecology, The Affiliated Hospital 2 of Nantong 
      University, Nantong, China.
FAU - Kang, Xinyi
AU  - Kang X
AD  - The Department of Obstetrics and Gynecology, The Affiliated Hospital 2 of Nantong 
      University, Nantong, China.
LA  - eng
GR  - MB2020003/2020 Nantong Health Commission Scientific Research Project (General 
      project B)/
GR  - FYX202029/Scientific research project of Jiangsu Maternal and Child Health 
      Association in 2020/
PT  - Journal Article
DEP - 20220619
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Diabetes Mellitus, Type 1
MH  - *Diabetes, Gestational/genetics
MH  - Female
MH  - Gene Expression Profiling
MH  - Genetic Markers
MH  - Humans
MH  - Pregnancy
MH  - Protein Interaction Maps/genetics
PMC - PMC9279970
OTO - NOTNLM
OT  - differentially expressed genes
OT  - functional analysis
OT  - gestational diabetes mellitus
OT  - protein-protein interaction network
COIS- The authors claim that they have no competing interests.
EDAT- 2022/06/21 06:00
MHDA- 2022/07/16 06:00
PMCR- 2022/06/19
CRDT- 2022/06/20 01:33
PHST- 2022/03/31 00:00 [revised]
PHST- 2021/08/22 00:00 [received]
PHST- 2022/04/24 00:00 [accepted]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/07/16 06:00 [medline]
PHST- 2022/06/20 01:33 [entrez]
PHST- 2022/06/19 00:00 [pmc-release]
AID - JCLA24515 [pii]
AID - 10.1002/jcla.24515 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2022 Jul;36(7):e24515. doi: 10.1002/jcla.24515. Epub 2022 Jun 
      19.