PMID- 35720075 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220716 IS - 1664-2295 (Print) IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 13 DP - 2022 TI - Hyperhomocysteinemia Is a Predictor for Poor Postoperative Angiogenesis in Adult Patients With Moyamoya Disease. PG - 902474 LID - 10.3389/fneur.2022.902474 [doi] LID - 902474 AB - BACKGROUND AND PURPOSES: The risk factors of poor postoperative angiogenesis in moyamoya disease (MMD) patients remain unknown. We aimed to investigate the association between hyperhomocysteinemia (HHcy) and postoperative angiogenesis of adult patients with MMD. METHODS: A total of 138 adult patients with MMD were prospectively recruited from July 1 to December 31, 2019. After excluding 10 patients accepting conservative therapy and 77 individuals without postoperative digital subtraction angiography (DSA), all 51 MMD patients were enrolled, and 28 patients received bilateral operations separately. Patients were grouped according to postoperative angiogenesis and HHcy presentation, respectively. Clinical data and laboratory examinations were compared. Potential risk factors were evaluated by univariate and multivariate logistic regression analysis. Nomogram was further performed. The biological functions of homocysteine (Hcy) were explored in vitro. RESULTS: Comparing to the normal, patients with poor postoperative angiogenesis were higher in serum Hcy (p = 0.004), HHcy ratio (p = 0.011), creatinine (Cr) (p < 0.001), uric acid (UA) (p = 0.036), Triglyceride (p = 0.001), high-density lipoprotein cholesterol (HDL-C) (p = 0.001), low-density lipoprotein cholesterol (LDL-C) (p = 0.009), ApoA (p = 0.022), apolipoprotein B (ApoB) (p = 0.013). Furthermore, HHcy was more common in men (p = 0.003) than women. Logistic analysis results showed that Hcy (OR = 0.817, 95% CI = 0.707-0.944, p = 0.006) was an independent risk factor. HHcy and Cr were significantly associated with poor postoperative angiogenesis in MMD patients. Further, Hcy could inhibit the proliferation, migration, and tube formation of human brain microvascular endothelial cells (HBMECs), which can be reversed by vascular endothelial growth factor (VEGF). CONCLUSION: The HHcy was significantly correlated with poor postoperative angiogenesis in adult patients with MMD. Hcy significantly inhibits HBMECs proliferation, migration, and tube formation. Furthermore, VEGF could reverse the inhibition effect induced by Hcy. Lowering the level of Hcy may be beneficial for postoperative MMD patients. Focusing on the pathophysiology and mechanism of HHcy might help to guide postoperative clinical management. CI - Copyright (c) 2022 He, Ge, Ye, Liu, Wang, Wang, Zhang, Zhang and Zhao. FAU - He, Qiheng AU - He Q AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Ge, Peicong AU - Ge P AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Ye, Xun AU - Ye X AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Liu, Xingju AU - Liu X AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Wang, Jia AU - Wang J AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Wang, Rong AU - Wang R AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Zhang, Yan AU - Zhang Y AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Zhang, Dong AU - Zhang D AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Zhao, Jizong AU - Zhao J AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. LA - eng PT - Journal Article DEP - 20220602 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 PMC - PMC9201052 OTO - NOTNLM OT - angiogenesis OT - homocysteine OT - hyperhomocysteinemia OT - moyamoya disease OT - prognosis OT - risk factor COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/06/21 06:00 MHDA- 2022/06/21 06:01 PMCR- 2022/06/02 CRDT- 2022/06/20 03:37 PHST- 2022/03/23 00:00 [received] PHST- 2022/04/22 00:00 [accepted] PHST- 2022/06/20 03:37 [entrez] PHST- 2022/06/21 06:00 [pubmed] PHST- 2022/06/21 06:01 [medline] PHST- 2022/06/02 00:00 [pmc-release] AID - 10.3389/fneur.2022.902474 [doi] PST - epublish SO - Front Neurol. 2022 Jun 2;13:902474. doi: 10.3389/fneur.2022.902474. eCollection 2022.