PMID- 35722395
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 10
DP  - 2022 May
TI  - Deletion of the miR-144/451 cluster aggravates lethal sepsis-induced lung 
      epithelial oxidative stress and apoptosis.
PG  - 538
LID - 10.21037/atm-22-1024 [doi]
LID - 538
AB  - BACKGROUND: Sepsis is associated with a high mortality rate. A major cause of 
      death in sepsis patients is respiratory failure, which is characterized by 
      oxidative injury, epithelial apoptosis, and increased lung permeability. 
      MicroRNAs (miRs) are important regulators of sepsis progression. METHODS: This 
      study aimed to explore the role of miR-144/451 in sepsis in mice. Experimental 
      sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). RESULTS: 
      CLP significantly induced systemic inflammation, lung permeability, and lung 
      epithelial apoptosis with downregulated messenger RNA (mRNA) levels of 
      antioxidant enzymes. The miR-144/451 knockout mice had a lower 48-hour survival 
      rate, higher plasma tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) 
      levels, and greater pulmonary permeability compared with wild-type mice after 
      CLP. CLP also markedly increased interstitial hemorrhage, collapsed more alveolar 
      sacs, and increased the number of terminal deoxynucleotidyl transferase dUTP 
      nick-end labeling (TUNEL)-positive and Bcl-2-associated X (Bax)-positive cells in 
      miR-144/451 knockout lung tissues, with elevated mRNA levels of Bax and reduced 
      activities of catalase (Cat), glutathione peroxidase 1(Gpx1). MiR-451 negatively 
      regulated 14-3-3zeta expression evidenced in miR-144/451 knockout lungs and the A549 
      cell line. In lipopolysaccharide (LPS)-induced A549 cells, miR-451 overexpression 
      remarkably suppressed the production of reactive oxygen species, inhibited cell 
      apoptosis, and enhanced levels of FoxO3 protein and related enzymes. CONCLUSIONS: 
      Deletion of the miR-144/451 cluster aggravated sepsis-induced oxidative injury of 
      lung epithelial cells.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Wu, Fan
AU  - Wu F
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, 
      Yangzhou, China.
FAU - Yuan, Xiaoling
AU  - Yuan X
AD  - Children Rehabilitation Department, Yangzhou Maternal and Child Health Hospital, 
      Yangzhou, China.
FAU - Liu, Weili
AU  - Liu W
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Intensive Care Unit, Affiliated Hospital of Yangzhou University, Yangzhou, China.
FAU - Meng, Lijun
AU  - Meng L
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Intensive Care Unit, Affiliated Hospital of Yangzhou University, Yangzhou, China.
FAU - Li, Xiuru
AU  - Li X
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Zhou, Shuting
AU  - Zhou S
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, 
      Yangzhou, China.
FAU - Fang, Lei
AU  - Fang L
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, 
      Yangzhou, China.
FAU - Yu, Duonan
AU  - Yu D
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
AD  - Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, 
      Yangzhou, China.
AD  - Intensive Care Unit, Affiliated Hospital of Yangzhou University, Yangzhou, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC9201188
OTO - NOTNLM
OT  - apoptosis
OT  - epithelial
OT  - miR-144/451
OT  - oxidative stress
OT  - sepsis
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-1024/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2022/06/21 06:00
MHDA- 2022/06/21 06:01
PMCR- 2022/05/01
CRDT- 2022/06/20 04:07
PHST- 2022/01/25 00:00 [received]
PHST- 2022/05/17 00:00 [accepted]
PHST- 2022/06/20 04:07 [entrez]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/06/21 06:01 [medline]
PHST- 2022/05/01 00:00 [pmc-release]
AID - atm-10-10-538 [pii]
AID - 10.21037/atm-22-1024 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 May;10(10):538. doi: 10.21037/atm-22-1024.