PMID- 35722408
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 10
DP  - 2022 May
TI  - Study on the intervention effect and mechanism of bacillus Calmette-Guerin 
      polysaccharide and nucleic acid injection on atopic dermatitis by targeting the 
      transient receptor potential vanilloid subtype 1 pathway.
PG  - 608
LID - 10.21037/atm-22-2101 [doi]
LID - 608
AB  - BACKGROUND: This study aimed to explore the mechanism of Bacillus Calmette-Guerin 
      polysaccharide and nucleic acid injection (BCG-PSN) targeting the transient 
      receptor potential vanilloid subtype 1 (TRPV1) pathway for atopic dermatitis (AD) 
      in mice. METHODS: Experiment 1: a total of 30 Kunming (KM) mice were randomized 
      into blank control, model, BCG-PSN low-dose (25 g/kg), BCG-PSN medium-dose (75 
      g/kg), BCG-PSN high-dose (225 g/kg), and positive drug (hydrocortisone 25 mg/kg) 
      control groups. The AD model mice were established by induction with 
      2,4-Dinitrochlorobenzene (DNCB). After treatment in groups, the symptom score and 
      scratching frequency in skin lesions were observed. The levels of immunoglobulin 
      E (IgE), interleukin (IL)-4, IL-31, and IL-13 in serum were detected, as well as 
      the levels of tumor necrosis factor-alpha (TNF-alpha), TRPV1, and nuclear factor (NF)-kappaB 
      p65 in skin lesions in each group. Experiment 2: the optimal dose of BCG-PSN in 
      Experiment 1 was adopted. A total of 20 KM mice were randomized into blank 
      control, model, BCG-PSN, and BCG-PSN + PAC (PAC-14028) groups. The symptom score 
      and scratching frequency in skin lesions were observed. The levels of IgE, IL-4, 
      IL-31, and IL-13 in serum were detected, as well as the levels of TNF-alpha and TRPV1 
      in skin lesions in each group. RESULTS: In Experiment 1, compared with the blank 
      control group, the ear tissues of mice in model groups developed AD, with 
      increased symptom score, scratching frequency, levels of IgE, IL-4, IL-31, and 
      IL-13 in serum and levels of TNF-alpha, TRPV1, and NF-kappaB p65 in skin lesions. 
      Compared with the model group, BCG-PSN low-dose, BCG-PSN medium-dose, BCG-PSN 
      high-dose, and positive drug control groups had reduced AD symptoms, decreased 
      symptom score, and decreased scratching frequency, with declined expression of 
      each inflammatory substance, including the greatest decrease in the medium-dose 
      group. In Experiment 2, after BCG-PSN was combined with PAC, the inflammation 
      indexes decreased compared with those in the model group, and increased compared 
      with those in the BCG-PSN group. CONCLUSIONS: Intramuscular BCG-PSN can target 
      the TRPV1 pathway, inhibit inflammation, and improve the symptoms of AD mice.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Wang, Xiufen
AU  - Wang X
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital of 
      Qiqihar Medical University, Qiqihar, China.
FAU - Wu, Di
AU  - Wu D
AD  - Scientific Research Section, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Duan, Tingting
AU  - Duan T
AD  - Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Lv, Shukun
AU  - Lv S
AD  - Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Cui, Liran
AU  - Cui L
AD  - Scientific Research Section, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Ding, Changrui
AU  - Ding C
AD  - Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
FAU - Xu, Yulong
AU  - Xu Y
AD  - Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical 
      University, Qiqihar, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC9201144
OTO - NOTNLM
OT  - Bacillus Calmette-Guerin polysaccharide and nucleic acid injection (BCG-PSN)
OT  - Transient receptor potential vanilloid subtype 1 (TRPV1)
OT  - atopic dermatitis (AD)
OT  - inflammation
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-2101/coif). All authors 
      report that this study was funded by Basic Scientific Research Business Expenses, 
      Research Project of Heilongjiang Provincial Undergraduate College, 2019 (No. 
      2019-KYYWF-1248). The authors have no other conflicts of interest to declare.
EDAT- 2022/06/21 06:00
MHDA- 2022/06/21 06:01
PMCR- 2022/05/01
CRDT- 2022/06/20 04:07
PHST- 2022/03/29 00:00 [received]
PHST- 2022/05/20 00:00 [accepted]
PHST- 2022/06/20 04:07 [entrez]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/06/21 06:01 [medline]
PHST- 2022/05/01 00:00 [pmc-release]
AID - atm-10-10-608 [pii]
AID - 10.21037/atm-22-2101 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 May;10(10):608. doi: 10.21037/atm-22-2101.