PMID- 35724900
OWN - NLM
STAT- MEDLINE
DCOM- 20220803
LR  - 20220803
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 215
DP  - 2022 Aug 31
TI  - Polysaccharide from Hemerocallis citrina Borani by subcritical water: 
      Bioactivity, purification, characterization, and anti-diabetic effects in T2DM 
      rats.
PG  - 169-183
LID - S0141-8130(22)01305-8 [pii]
LID - 10.1016/j.ijbiomac.2022.06.101 [doi]
AB  - Hemerocallis citrina Borani (daylily) has various health benefits. However, the 
      structural characterization and hypoglycemic effects of its polysaccharide remain 
      unclear. Here, we first report for the first time, the effects of subcritical 
      water extraction temperature on bioactivity of Hemerocallis citrina Borani 
      polysaccharide (HCBP). HCBP extracted at 160  degrees C had better scavenging ability of 
      free-radical and pancreatic lipase inhibition. This study aimed to investigate 
      the structural characterization and anti-diabetic effects of aforementioned HCBP. 
      HCBP was separated into three fractions using a DEAE-Sepharose Fast Flow. HCBP-1 
      was the major component with lower molecular weight, HCBP-2 was the least 
      abundant component, HCBP-3 was the component with higher uronic acid and 
      molecular weight. HCBP treatment significantly decreased the concentrations of 
      fasting blood glucose, insulin, total cholesterol, triglyceride, and low density 
      lipoprotein, as well as improved glucose intolerance and insulin resistance in 
      type 2 diabetes mellitus (T2DM) rats. HCBP also protected the kidneys and 
      pancreatic organs by histopathology and immunohistochemical analyses in T2DM 
      rats. In addition, HCBP significantly increased the antioxidant enzymes 
      activities and decreased the level of malonaldehyde to alleviated the oxidative 
      stress injury. The results indicate HCBP extracted by subcritical water is 
      promising functional food ingredients and could be used in T2DM treatment.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Ti, Yongrui
AU  - Ti Y
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Wang, Weizhen
AU  - Wang W
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Zhang, Yanli
AU  - Zhang Y
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Ban, Yuqian
AU  - Ban Y
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Wang, Xiaoxiao
AU  - Wang X
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Wang, Peng
AU  - Wang P
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Song, Zihan
AU  - Song Z
AD  - Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China. Electronic address: songzihan@caas.cn.
LA  - eng
PT  - Journal Article
DEP - 20220617
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Polysaccharides)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animals
MH  - Antioxidants/analysis/pharmacology
MH  - Blood Glucose/analysis
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Dietary Carbohydrates
MH  - *Hemerocallis/chemistry
MH  - Hypoglycemic Agents/pharmacology
MH  - Polysaccharides/pharmacology/therapeutic use
MH  - Rats
MH  - Water
OTO - NOTNLM
OT  - Characterization
OT  - Hemerocallis citrina Borani polysaccharide
OT  - Hypoglycemic
OT  - Purification
OT  - Subcritical water
OT  - T2DM rats
EDAT- 2022/06/21 06:00
MHDA- 2022/08/04 06:00
CRDT- 2022/06/20 19:27
PHST- 2022/03/12 00:00 [received]
PHST- 2022/05/31 00:00 [revised]
PHST- 2022/06/12 00:00 [accepted]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/08/04 06:00 [medline]
PHST- 2022/06/20 19:27 [entrez]
AID - S0141-8130(22)01305-8 [pii]
AID - 10.1016/j.ijbiomac.2022.06.101 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 Aug 31;215:169-183. doi: 
      10.1016/j.ijbiomac.2022.06.101. Epub 2022 Jun 17.