PMID- 35725243
OWN - NLM
STAT- MEDLINE
DCOM- 20230106
LR  - 20230127
IS  - 2059-8696 (Electronic)
IS  - 2059-8688 (Print)
IS  - 2059-8688 (Linking)
VI  - 7
IP  - 6
DP  - 2022 Dec
TI  - Inhibition of the postsynaptic density protein 95 on the protective effect of 
      Ang-(1-7)-Mas on cerebral ischaemia injury.
PG  - 500-509
LID - 10.1136/svn-2021-001396 [doi]
AB  - BACKGROUND: Postsynaptic density protein-95 (PSD95) plays an important role in 
      cerebral ischaemia injury, but its mechanism needs further research. This study 
      aimed to explore the role of PSD95 in (Ang-(1-7))-Mas-mediated cerebral ischaemia 
      protection and its regulatory mechanism. METHODS: Oxygen-glucose deprivation 
      (OGD) neuron and rat middle cerebral artery occlusion (MCAO) models were used as 
      in vitro and in vivo models, respectively. TAT-MAS9C was used to disrupt the 
      interaction between PSD95 and Mas. The recombinant PSD95 adenovirus (Ad-PSD95) 
      was used to overexpress PSD95 in neurons. RESULTS: Results showed that in OGD 
      neurons, Ang-(1-7) could promote cell viability; reduce cell apoptosis; reduce 
      the cell membrane localisation of Mas; upregulate the expression levels of pAKT, 
      bcl-2 and I-kappaB; and downregulate the expression levels of Bax, pI-kappaB, tumour 
      necrosis factor alpha and interleukin-1beta. TAT-MAS9C could enhance the 
      aforementioned effects of Ang-(1-7). However, the PSD95 overexpression inhibited 
      the aforementioned effects of Ang-(1-7). In the MCAO rat model, the 
      2,3,5-triphenyltetrazolium chloride (TTC) staining showed that Ang-(1-7) reduced 
      the infarct volume. The Morris water maze test showed that the number of 
      crossings over the platform area in the Ang-(1-7) group was significantly 
      increased. TAT-MAS9C could promote the protective effect of Ang-(1-7). 
      CONCLUSIONS: Results suggested that PSD95 alleviated the activation of AKT and 
      the inhibition of nuclear factor kappa B signalling pathway mediated by the 
      Ang-(1-7)-Mas complex, thereby reducing neuronal activity, increasing apoptosis 
      and inhibiting the Ang-(1-7)-Mas-mediated cerebral ischaemia protection.
CI  - (c) Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No 
      commercial re-use. See rights and permissions. Published by BMJ.
FAU - Zhao, Bingrui
AU  - Zhao B
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Wang, Zhe
AU  - Wang Z
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Liang, Xinyue
AU  - Liang X
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - College of Intelligence and Information Engineering, Shandong University of 
      Traditional Chinese Medicine, Jinan, Shandong, China.
FAU - Lin, Kai
AU  - Lin K
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Yuan, Lihua
AU  - Yuan L
AD  - Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of 
      Sciences, Suzhou, Jiangsu, China.
FAU - Jiang, Jing
AU  - Jiang J
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Xu, Cong
AU  - Xu C
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Zhang, Daolai
AU  - Zhang D
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.
FAU - Sun, Yeying
AU  - Sun Y
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China 
      bian_1005@163.com sunyy@bzmc.edu.cn.
FAU - Bian, Weihua
AU  - Bian W
AUID- ORCID: 0000-0003-2369-2073
AD  - School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China 
      bian_1005@163.com sunyy@bzmc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220620
PL  - England
TA  - Stroke Vasc Neurol
JT  - Stroke and vascular neurology
JID - 101689996
RN  - 0 (Disks Large Homolog 4 Protein)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Disks Large Homolog 4 Protein/metabolism
MH  - *Brain Ischemia/metabolism
MH  - Signal Transduction
MH  - Apoptosis
MH  - Infarction, Middle Cerebral Artery/pathology
MH  - *Brain Injuries
PMC - PMC9811564
OTO - NOTNLM
OT  - brain
OT  - cerebral infarction
OT  - stroke
COIS- Competing interests: None declared.
EDAT- 2022/06/21 06:00
MHDA- 2023/01/07 06:00
PMCR- 2022/06/20
CRDT- 2022/06/20 21:07
PHST- 2021/11/03 00:00 [received]
PHST- 2022/04/04 00:00 [accepted]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2023/01/07 06:00 [medline]
PHST- 2022/06/20 21:07 [entrez]
PHST- 2022/06/20 00:00 [pmc-release]
AID - svn-2021-001396 [pii]
AID - 10.1136/svn-2021-001396 [doi]
PST - ppublish
SO  - Stroke Vasc Neurol. 2022 Dec;7(6):500-509. doi: 10.1136/svn-2021-001396. Epub 
      2022 Jun 20.