PMID- 35725316
OWN - NLM
STAT- MEDLINE
DCOM- 20220622
LR  - 20220624
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 57
IP  - 6
DP  - 2022 Jun 7
TI  - [Study on the effect and mechanisms of SIGLEC-15 on laryngeal squamous cell 
      carcinoma].
PG  - 724-731
LID - 10.3760/cma.j.cn115330-20210705-00424 [doi]
AB  - Objective: To investigate the effect of sialic acid combined with 
      immunoglobulin-like lectin 15 (SIGLEC-15) on laryngeal squamous cell carcinoma 
      (LSCC) and underlying mechanisms. Methods: The Cancer Genome Atlas (TCGA), Cancer 
      Cell Line Encyclopedia (CCLE) and Gene Expression Profiling Interactive Analysis 
      (GEPIA2) databases were used for bioinformatics analysis. Cell Counting Kit-8 
      (CCK8), flow cytometry, and Transwell method were used respectively to detect 
      proliferation, apoptosis, cell cycle, metastasis and invasion behaviors of the 
      cells. Gene chip method was used for detecting up-regulated and down-regulated 
      genes and performing enrichment analysis of differential genes. Western Blotting 
      (WB) and Quantitative Real-time PCR (qPCR) were used to detect the expressions of 
      proteins. Tumor formation experiments in nude mice were used to detect the effect 
      of SIGLEC-15 on the growth of transplanted tumors. Wilcoxon rank sum test, t-test 
      and Log-rank test were used for statistical analysis. Results: SIGLEC-15 was 
      highly expressed in laryngeal squamous cell carcinoma and closely related to life 
      in being. TU686(SIGLEC-15-)with low expression of SIGLEC-15 was constructed. 
      Compared to TU686(SIGLEC-15+), TU686(SIGLEC-15-)showed significantly reduced 
      activities of proliferation (48 h: 1.32+/-0.23 vs. 2.56+/-0.37, t=6.59, P<0.05), 
      migration (1 036.52+/-51.22 vs. 1 819.62+/-180.24, t=7.22, P<0.05) and invasion 
      (469.21+/-112.25 vs. 961.45+/-102.03, t=7.85, P<0.05); early increased apoptosis 
      ((23.27+/-1.12)% vs. (5.64+/-1.61)%, t=11.32, P<0.05); blocked cell cycle at G0/G1 
      ((59.32+/-3.65)% vs. (35.46+/-3.57)%, t=9.85, P<0.05); the knockdown of SIGLEC-15 
      resulted in up-regulation of 864 genes, down-regulation of 357 genes, with 
      significant changes in molecules of cell cycle, apoptosis and JAK/STAT signal 
      pathways, and the expressions of p-JAK2, p-STAT3, Caspase-3, Bad, Bcl-2, and 
      Cyclin d1 proteins. Tumor formation experiments in nude mice showed that at 8 
      weeks after the tumors were implanted, the growth transplanted tumors of TU686 
      SIGLEC-15-cell group was slower than that of TU686 SIGLEC-15+cell group, with 
      significant difference in the mean tumor weights between two groups 
      ((0.382+/-0.054) g vs. (1.277+/-0.126) g, t=8.44, P<0.05), while the expression of 
      SIGLEC-15 was lower in the transplanted tumors of SIGLEC-15 knockdown group 
      compared to control group, with significant difference(11.29+/-2.17 vs. 36.25+/-7.56, 
      t=9.28, P<0.05). Conclusion: SIGLEC-15 is highly expressed in LSCC and can 
      promote the progression of LSCC through the JAK2-STAT3 pathway.
FAU - Li, S H
AU  - Li SH
AD  - Department of Otolaryngology, Zhoukou Central Hospital, Zhoukou 466000, Henan 
      Province, China.
FAU - Sun, J
AU  - Sun J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, the First Affiliated 
      Hospital of Zhengzhou University, Zhengzhou 450052, China.
FAU - Chen, Wenming
AU  - Chen W
AD  - Department of Otolaryngology, Henan Hospital of Traditional Chinese Medicine, 
      Zhengzhou 450002, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Sialic Acid Binding Immunoglobulin-like Lectins)
RN  - 0 (Siglec-15 protein, mouse)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - *Carcinoma, Squamous Cell/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Immunoglobulins/genetics/metabolism
MH  - *Laryngeal Neoplasms/pathology
MH  - *Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Nude
MH  - *Sialic Acid Binding Immunoglobulin-like Lectins/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
EDAT- 2022/06/21 06:00
MHDA- 2022/06/23 06:00
CRDT- 2022/06/20 23:04
PHST- 2022/06/20 23:04 [entrez]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/06/23 06:00 [medline]
AID - 10.3760/cma.j.cn115330-20210705-00424 [doi]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2022 Jun 7;57(6):724-731. doi: 
      10.3760/cma.j.cn115330-20210705-00424.