PMID- 35725615
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Jun 20
TI  - Circular RNA EPB41 expression predicts unfavorable prognoses in NSCLC by 
      regulating miR-486-3p/eIF5A axis-mediated stemness.
PG  - 219
LID - 10.1186/s12935-022-02618-7 [doi]
LID - 219
AB  - Dysregulation of circular RNAs (circRNAs) has recently been found to play an 
      important role in the progression and development of cancers such as non-small 
      cell lung cancer (NSCLC). Yet the functions of many circRNAs in NSCLC remain 
      unclear. In this study, the circRNA expression profiles in NSCLC tumor tissues 
      and adjacent non-tumorous tissues were detected by high-throughput sequencing. 
      Bioinformatics analyses, the dual-luciferase reporter system, fluorescence in 
      situ hybridization (FISH) and miRNA/mRNA high-throughput sequencing were used to 
      identify circ-EPB41 and its downstream target. The subcutaneous tumor/caudal vein 
      transfer mouse model was used for tumor growth and invasion analysis. The results 
      show that the circ-EPB41 was upregulated in NSCLC tissues and cell lines. 
      Increased circ-EPB41 expression in NSCLC was significantly correlated with 
      malignant characteristics, and positive to post-surgical overall survival of 
      NSCLC patients. Reduced circ-EPB41 expression in NSCLC decreased cell 
      proliferation and invasion in both in vitro and in vivo experiments. The 
      miRNA/mRNA high-throughput sequencing suggested that downregulation of circ-EPB41 
      promoted microRNA (miR)-486-3p and suppressed eukaryotic translation initiation 
      factor 5A (eIF5A) expression. Luciferase reporter experiments confirmed that 
      miR-486-3p/eIF5A were downstream targets of circ-EPB41. In addition, we also 
      found that downregulation of circ-EPB41 suppressed self-renewal and decreased 
      expression of stemness markers SOX2, OCT-4, Nanog and CD133 by sponging 
      miR-486-3p to enhance eIF5A expression. Taken togeter, these data revealed the 
      important role of circ-EPB41 in regulating NSCLC cell invasion and proliferation 
      by modifying miR-486-3p/eIF5A axis-mediated stemness. We believe our study 
      provides a novel perspective regarding the role of circRNAs in NSCLC progression.
CI  - (c) 2022. The Author(s).
FAU - Jin, Mingming
AU  - Jin M
AUID- ORCID: 0000-0003-3574-2410
AD  - Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and 
      Health Sciences, 279 Zhouzhu Road, Pudong New Area, Shanghai, 201318, People's 
      Republic of China.
AD  - Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's 
      Republic of China.
FAU - Liu, Xiyu
AU  - Liu X
AD  - Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and 
      Health Sciences, 279 Zhouzhu Road, Pudong New Area, Shanghai, 201318, People's 
      Republic of China.
AD  - Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's 
      Republic of China.
FAU - Wu, Yue
AU  - Wu Y
AD  - Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and 
      Health Sciences, 279 Zhouzhu Road, Pudong New Area, Shanghai, 201318, People's 
      Republic of China.
AD  - Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's 
      Republic of China.
FAU - Lou, Yuqing
AU  - Lou Y
AD  - Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and 
      Health Sciences, 279 Zhouzhu Road, Pudong New Area, Shanghai, 201318, People's 
      Republic of China.
AD  - Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's 
      Republic of China.
FAU - Li, Xue
AU  - Li X
AD  - Health School Attached to Shanghai University of Medicine and Health Sciences, 
      Shanghai, 200237, People's Republic of China. lixue5303@126.com.
FAU - Huang, Gang
AU  - Huang G
AD  - Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and 
      Health Sciences, 279 Zhouzhu Road, Pudong New Area, Shanghai, 201318, People's 
      Republic of China. huanggang@sumhs.edu.cn.
AD  - Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's 
      Republic of China. huanggang@sumhs.edu.cn.
LA  - eng
GR  - 81830052/National Natural Science Foundation of China/
GR  - 82003142/National Natural Science Foundation of China/
GR  - 81530053/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220620
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC9210757
OTO - NOTNLM
OT  - Cancer stem cells
OT  - Circ-EPB41
OT  - Non-small cell lung cancer
OT  - eIF5A
OT  - miR-486-3p
COIS- The authors declare that they have no competing interests.
EDAT- 2022/06/21 06:00
MHDA- 2022/06/21 06:01
PMCR- 2022/06/20
CRDT- 2022/06/20 23:47
PHST- 2021/09/03 00:00 [received]
PHST- 2022/05/20 00:00 [accepted]
PHST- 2022/06/20 23:47 [entrez]
PHST- 2022/06/21 06:00 [pubmed]
PHST- 2022/06/21 06:01 [medline]
PHST- 2022/06/20 00:00 [pmc-release]
AID - 10.1186/s12935-022-02618-7 [pii]
AID - 2618 [pii]
AID - 10.1186/s12935-022-02618-7 [doi]
PST - epublish
SO  - Cancer Cell Int. 2022 Jun 20;22(1):219. doi: 10.1186/s12935-022-02618-7.