PMID- 35727557
OWN - NLM
STAT- MEDLINE
DCOM- 20220623
LR  - 20220802
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2498
DP  - 2022
TI  - Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In 
      Situ Hybridization (FISH).
PG  - 363-372
LID - 10.1007/978-1-0716-2313-8_21 [doi]
AB  - The opportunity to map genes and noncoding DNA sequences on the chromosomes by 
      fluorescence in situ hybridization (FISH) has greatly enhanced the potential for 
      fish karyotyping and comparative cytogenetics. The use of FISH allowed for 
      significant advances in our understanding of the fish genome architecture, 
      especially when applied to the study of the repetitive component of the genome, 
      that is generally underestimated in the bioinformatic assembly. Here we provide a 
      step-by-step protocol for FISH of repeated sequences onto chromosomes of fish 
      species.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Ghigliotti, Laura
AU  - Ghigliotti L
AD  - National Research Council of Italy (CNR), Institute for the Study of the 
      Anthropic Impacts and the Sustainability of the Marine Environment (IAS), Genoa, 
      Italy. laura.ghigliotti@cnr.it.
FAU - Auvinet, Juliette
AU  - Auvinet J
AD  - Department of Marine and Environmental Sciences, Northeastern University, Marine 
      Science Center, Nahant, MA, USA.
FAU - Pisano, Eva
AU  - Pisano E
AD  - National Research Council of Italy (CNR), Institute for the Study of the 
      Anthropic Impacts and the Sustainability of the Marine Environment (IAS), Genoa, 
      Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - *Repetitive Sequences, Nucleic Acid
OTO - NOTNLM
OT  - Fish
OT  - Fluorescence microscopy
OT  - Genome architecture
OT  - Repetitive sequence
OT  - Ribosomal gene
OT  - Transposable element
EDAT- 2022/06/22 06:00
MHDA- 2022/06/24 06:00
CRDT- 2022/06/21 11:24
PHST- 2022/06/21 11:24 [entrez]
PHST- 2022/06/22 06:00 [pubmed]
PHST- 2022/06/24 06:00 [medline]
AID - 10.1007/978-1-0716-2313-8_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2498:363-372. doi: 10.1007/978-1-0716-2313-8_21.