PMID- 35728279
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220729
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 619
DP  - 2022 Sep 3
TI  - 2D-DIGE based urinary proteomics and functional enrichment studies to reveal 
      novel Mycobacterium tuberculosis and human protein biomarker candidates for 
      pulmonary tuberculosis.
PG  - 15-21
LID - S0006-291X(22)00862-2 [pii]
LID - 10.1016/j.bbrc.2022.06.021 [doi]
AB  - In the absence of a sensitive and specific diagnostic modality capable of 
      detecting all forms of tuberculosis (TB), proteomics may identify specific 
      Mycobacterium tuberculosis (M.tb) proteins in urine, with a potential as 
      biomarkers. To identify candidate biomarkers for TB, proteome profile of urine 
      from pulmonary TB patients was compared with non-disease controls (NDC) and 
      disease controls (DC, Streptococcus pneumonia infected patients) using a 
      combination of two-dimensional difference gel electrophoresis (2D-DIGE) and 
      liquid chromatography tandem mass spectrometry (LCMS/MS). Eleven differentially 
      expressed host proteins and Eighteen high abundant M.tb proteins were identified. 
      Protein-protein interactome (PPI) and functional enrichment analyses like Gene 
      Ontologies, Reactome pathway etc. demonstrated that the human proteins mainly 
      belong to extracellular space and show physiological pathways for immune response 
      and hematological disorders. Whereas, M.tb proteins belong to the cell periphery, 
      plasma membrane and cell wall, and demonstrated catalytic, nucleotide binding and 
      ATPase activities along with other functional processes. The study findings 
      provide valuable inputs about the biomarkers of TB and shed light on the probable 
      disease consequences as an outcome of the bacterial pathogenicity.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Kaushik, Amit
AU  - Kaushik A
AD  - Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Bandyopadhyay, Sabyasachi
AU  - Bandyopadhyay S
AD  - Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Porwal, Chhavi
AU  - Porwal C
AD  - Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Srinivasan, Alagiri
AU  - Srinivasan A
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Rukmangadachar, Lokesh A
AU  - Rukmangadachar LA
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Hariprasad, Gururao
AU  - Hariprasad G
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Kola, Srujana
AU  - Kola S
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Kataria, Jitender
AU  - Kataria J
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Singh, Urvashi B
AU  - Singh UB
AD  - Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 
      110029, India. Electronic address: drurvashi@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220609
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Humans
MH  - *Mycobacterium tuberculosis/metabolism
MH  - Proteomics/methods
MH  - Tandem Mass Spectrometry/methods
MH  - *Tuberculosis/microbiology
MH  - *Tuberculosis, Pulmonary/diagnosis
MH  - Two-Dimensional Difference Gel Electrophoresis
OTO - NOTNLM
OT  - 2D-DIGE
OT  - Biomarker
OT  - LC-MS/MS
OT  - Mycobacterium tuberculosis
OT  - Proteomics
OT  - Urine
COIS- Declaration of competing interest The authors declare that there is no conflict 
      of interests in this work.
EDAT- 2022/06/22 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/06/21 18:13
PHST- 2022/05/05 00:00 [received]
PHST- 2022/06/01 00:00 [revised]
PHST- 2022/06/08 00:00 [accepted]
PHST- 2022/06/22 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
PHST- 2022/06/21 18:13 [entrez]
AID - S0006-291X(22)00862-2 [pii]
AID - 10.1016/j.bbrc.2022.06.021 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 Sep 3;619:15-21. doi: 
      10.1016/j.bbrc.2022.06.021. Epub 2022 Jun 9.