PMID- 35728770
OWN - NLM
STAT- MEDLINE
DCOM- 20220707
LR  - 20220918
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Linking)
VI  - 265
DP  - 2022 Aug 15
TI  - Proteomic profile of Candida albicans biofilm.
PG  - 104661
LID - S1874-3919(22)00185-3 [pii]
LID - 10.1016/j.jprot.2022.104661 [doi]
AB  - Candida albicans biofilms are characterized by structural and cellular 
      heterogeneity that confers antifungal resistance and immune evasion. Despite 
      this, biofilm formation remains poorly understood. In this study, we used 
      proteomic analysis to understand biofilm formation in C. albicans related to 
      morphophysiological and architectural features. LC-MS/MS analysis revealed that 
      64 proteins were significantly modulated, of which 31 were upregulated and 33 
      were downregulated. The results indicate that metabolism (25 proteins), gene 
      expression (13 proteins), stress response (7 proteins), and cell wall (5 
      proteins) composition are modulated. The rate of oxidative phosphorylation 
      (OxPhos) and biosynthesis of UDP-N-acetylglucosamine, vitamin B6, and thiamine 
      increased, while the rate of methionine biosynthesis decreased. There was a 
      significant modification of the cell wall architecture due to higher levels of 
      Sun41, Pir1 and Csh1 and increased glycosylation of proteins. It was observed 
      that C. albicans induces hyphal growth by upregulating the expression of genes 
      involved in cAMP-PKA and MAPK pathways. This study is significant in that it 
      suggests an increase in OxPhos and alteration of cell wall architecture that 
      could be contributing to the recalcitrance of C. albicans cells growing in 
      biofilms. Nevertheless, a deeper investigation is needed to explore it further. 
      SIGNIFICANCE: Candida sps is included in the list of pathogens with potential 
      drug resistance threat due to the increased frequency especially colonization of 
      medical devices, and tissues among the patients, in recent years. Significance of 
      our study is that we are reporting traits like modulation in cell wall 
      composition, amino acid and vitamin biosynthesis and importantly energy 
      generation (OxPhos) etc. These traits could be conferring antifungal resistance, 
      host immune evasion etc. and thus survival, in addition to facilitating biofilm 
      formation. These findings are expected to prime the further studies on devising 
      potent strategy against biofilm growth among the patients.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Abdulghani, Mazen
AU  - Abdulghani M
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Iram, Rasiqua
AU  - Iram R
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Chidrawar, Priti
AU  - Chidrawar P
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Bhosle, Kajal
AU  - Bhosle K
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Kazi, Rubina
AU  - Kazi R
AD  - Division of Biochemical Sciences, CSIR-NCL, Pune 8, MS, India.
FAU - Patil, Rajendra
AU  - Patil R
AD  - Department of Biotechnology, Savitribai Phule Pune University, Ganeshkhind, Pune 
      411007, MS, India.
FAU - Kharat, Kiran
AU  - Kharat K
AD  - Department of Biotechnology, Deogiri College, Aurangabad, MS, India.
FAU - Zore, Gajanan
AU  - Zore G
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India. Electronic address: zoreg1@yahoo.co.in.
LA  - eng
PT  - Journal Article
DEP - 20220618
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Antifungal Agents)
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - *Antifungal Agents/pharmacology
MH  - Biofilms
MH  - *Candida albicans/metabolism
MH  - Chromatography, Liquid
MH  - Fungal Proteins/metabolism
MH  - Humans
MH  - Proteomics
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Biofilm
OT  - Candida albicans
OT  - Cell wall
OT  - LC-MS/MS
OT  - Metabolism
OT  - Proteomics
EDAT- 2022/06/22 06:00
MHDA- 2022/07/08 06:00
CRDT- 2022/06/21 19:26
PHST- 2021/09/15 00:00 [received]
PHST- 2022/04/06 00:00 [revised]
PHST- 2022/06/08 00:00 [accepted]
PHST- 2022/06/22 06:00 [pubmed]
PHST- 2022/07/08 06:00 [medline]
PHST- 2022/06/21 19:26 [entrez]
AID - S1874-3919(22)00185-3 [pii]
AID - 10.1016/j.jprot.2022.104661 [doi]
PST - ppublish
SO  - J Proteomics. 2022 Aug 15;265:104661. doi: 10.1016/j.jprot.2022.104661. Epub 2022 
      Jun 18.