PMID- 35729017
OWN - NLM
STAT- MEDLINE
DCOM- 20221004
LR  - 20221004
IS  - 1521-4028 (Electronic)
IS  - 0233-111X (Linking)
VI  - 62
IP  - 10
DP  - 2022 Oct
TI  - Investigation of the effects of antimicrobial and anti-biofilm peptide IDR1018 
      and chitosan nanoparticles on ciprofloxacin-resistant Escherichia coli.
PG  - 1229-1240
LID - 10.1002/jobm.202200156 [doi]
AB  - Peptide IDR1018 and chitosan nanoparticles (CNs) showed antimicrobial and 
      anti-biofilm activity against bacteria. In this study, the antimicrobial effects 
      of peptide IDR1018 and CNs were evaluated on 50 clinical isolates of 
      uropathogenic Escherichia coli (UPEC) resistant to ciprofloxacin. Ion gelation 
      method was applied for CNs synthesis. Scanning electron microscope (SEM) and 
      dynamic light scattering (DLS) were utilized to evaluate the nanoparticles. 
      Antimicrobial and synergistic activity of peptide IDR1018 and CNs with 
      ciprofloxacin were evaluated by microtiter broth dilution method. The 
      checkerboard test was used to investigate the antimicrobial effects of IDR1018 
      and CNS in combination with ciprofloxacin. Anti-biofilm effect of ciprofloxacin, 
      peptide IDR1018, and CNs was evaluated using crystal violet method. Fourteen 
      (28%), 21 (42%), and 15 (30%) of clinical isolates produced strong, moderate, and 
      weak biofilm, respectively. The CNs were spherical and uniform under electron 
      microscopy with an average diameter of 246 nm. The minimum inhibitory 
      concentration (MIC) values were 16-128, 20-40, and 375-750 (microg/ml) for 
      ciprofloxacin, peptide IDR1018, and CNs, respectively. Fractional inhibitory 
      concentration (FIC) analysis indicated a synergistic effect of ciprofloxacin in 
      combination with peptide IDR1018, but in combination with CNs, this antibiotic 
      showed an additive effect. Our results revealed that peptide IDR1018 and CNs have 
      antimicrobial properties on UPEC isolates. Biofilm inhibition and biofilm 
      eradication of clinical isolate were shown by peptide IDR1018 and CNs in a 
      concentration-dependent manner. The antimicrobial agents alone and in combination 
      decreased the number of viable bacteria in the biofilms. Therefore, these 
      components seem to be a treating approach against biofilm-forming UPEC isolates.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Haji Hossein Tabrizi, Aida
AU  - Haji Hossein Tabrizi A
AD  - Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, 
      Iran.
FAU - Habibi, Mehri
AU  - Habibi M
AUID- ORCID: 0000-0003-2191-7873
AD  - Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
FAU - Foroohi, Fatemeh
AU  - Foroohi F
AD  - Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, 
      Iran.
FAU - Mohammadian, Taher
AU  - Mohammadian T
AD  - Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, 
      Iran.
FAU - Asadi Karam, Mohammad Reza
AU  - Asadi Karam MR
AD  - Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220621
PL  - Germany
TA  - J Basic Microbiol
JT  - Journal of basic microbiology
JID - 8503885
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (innate defense regulating peptide 1018)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - 9012-76-4 (Chitosan)
RN  - J4Z741D6O5 (Gentian Violet)
SB  - IM
MH  - Anti-Bacterial Agents/chemistry
MH  - Antimicrobial Cationic Peptides
MH  - Biofilms
MH  - *Chitosan/chemistry/pharmacology
MH  - Ciprofloxacin/pharmacology/therapeutic use
MH  - *Escherichia coli Infections/drug therapy
MH  - Gentian Violet
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - *Nanoparticles
MH  - *Uropathogenic Escherichia coli
OTO - NOTNLM
OT  - antibiotic resistance
OT  - chitosan nanoparticles
OT  - peptide IDR1018 
OT  - urinary tract infection
OT  - uropathogenic Escherichia coli
EDAT- 2022/06/22 06:00
MHDA- 2022/10/05 06:00
CRDT- 2022/06/21 22:04
PHST- 2022/05/21 00:00 [revised]
PHST- 2022/03/15 00:00 [received]
PHST- 2022/05/27 00:00 [accepted]
PHST- 2022/06/22 06:00 [pubmed]
PHST- 2022/10/05 06:00 [medline]
PHST- 2022/06/21 22:04 [entrez]
AID - 10.1002/jobm.202200156 [doi]
PST - ppublish
SO  - J Basic Microbiol. 2022 Oct;62(10):1229-1240. doi: 10.1002/jobm.202200156. Epub 
      2022 Jun 21.