PMID- 35731940
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220920
IS  - 1759-6653 (Electronic)
IS  - 1759-6653 (Linking)
VI  - 14
IP  - 7
DP  - 2022 Jul 2
TI  - Pangenome Evolution in Environmentally Transmitted Symbionts of Deep-Sea Mussels 
      Is Governed by Vertical Inheritance.
LID - 10.1093/gbe/evac098 [doi]
LID - evac098
AB  - Microbial pangenomes vary across species; their size and structure are determined 
      by genetic diversity within the population and by gene loss and horizontal gene 
      transfer (HGT). Many bacteria are associated with eukaryotic hosts where the host 
      colonization dynamics may impact bacterial genome evolution. Host-associated 
      lifestyle has been recognized as a barrier to HGT in parentally transmitted 
      bacteria. However, pangenome evolution of environmentally acquired symbionts 
      remains understudied, often due to limitations in symbiont cultivation. Using 
      high-resolution metagenomics, here we study pangenome evolution of two 
      co-occurring endosymbionts inhabiting Bathymodiolus brooksi mussels from a single 
      cold seep. The symbionts, sulfur-oxidizing (SOX) and methane-oxidizing (MOX) 
      gamma-proteobacteria, are environmentally acquired at an early developmental 
      stage and individual mussels may harbor multiple strains of each symbiont 
      species. We found differences in the accessory gene content of both symbionts 
      across individual mussels, which are reflected by differences in symbiont strain 
      composition. Compared with core genes, accessory genes are enriched in genome 
      plasticity functions. We found no evidence for recent HGT between both symbionts. 
      A comparison between the symbiont pangenomes revealed that the MOX population is 
      less diverged and contains fewer accessory genes, supporting that the MOX 
      association with B. brooksi is more recent in comparison to that of SOX. Our 
      results show that the pangenomes of both symbionts evolved mainly by vertical 
      inheritance. We conclude that genome evolution of environmentally transmitted 
      symbionts that associate with individual hosts over their lifetime is affected by 
      a narrow symbiosis where the frequency of HGT is constrained.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of Society 
      for Molecular Biology and Evolution.
FAU - Romero Picazo, Devani
AU  - Romero Picazo D
AUID- ORCID: 0000-0002-0174-9115
AD  - Genomic Microbiology Group, Institute of General Microbiology, 
      Christian-Albrechts University, 24118 Kiel, Germany.
FAU - Werner, Almut
AU  - Werner A
AUID- ORCID: 0000-0003-1570-4329
AD  - Genomic Microbiology Group, Institute of General Microbiology, 
      Christian-Albrechts University, 24118 Kiel, Germany.
FAU - Dagan, Tal
AU  - Dagan T
AUID- ORCID: 0000-0002-9042-192X
AD  - Genomic Microbiology Group, Institute of General Microbiology, 
      Christian-Albrechts University, 24118 Kiel, Germany.
FAU - Kupczok, Anne
AU  - Kupczok A
AUID- ORCID: 0000-0001-5237-1899
AD  - Genomic Microbiology Group, Institute of General Microbiology, 
      Christian-Albrechts University, 24118 Kiel, Germany.
AD  - Max Planck Institute for Marine Microbiology, 28359 Bremen, Germany.
AD  - Bioinformatics Group, Wageningen University & Research, 6708PB Wageningen, The 
      Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genome Biol Evol
JT  - Genome biology and evolution
JID - 101509707
RN  - 70FD1KFU70 (Sulfur)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics
MH  - Gene Transfer, Horizontal
MH  - Genome, Bacterial
MH  - Methane
MH  - *Mytilidae/genetics/microbiology
MH  - Phylogeny
MH  - Sulfur
MH  - Symbiosis/genetics
PMC - PMC9260185
OTO - NOTNLM
OT  - accessory genome
OT  - high-resolution metagenomics
OT  - horizontal gene transfer
OT  - pangenome
EDAT- 2022/06/23 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/06/22
CRDT- 2022/06/22 14:52
PHST- 2022/06/18 00:00 [accepted]
PHST- 2022/06/23 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/06/22 14:52 [entrez]
PHST- 2022/06/22 00:00 [pmc-release]
AID - 6613374 [pii]
AID - evac098 [pii]
AID - 10.1093/gbe/evac098 [doi]
PST - ppublish
SO  - Genome Biol Evol. 2022 Jul 2;14(7):evac098. doi: 10.1093/gbe/evac098.