PMID- 35732879
OWN - NLM
STAT- MEDLINE
DCOM- 20220624
LR  - 20221114
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Jun 22
TI  - Homologous chromosome associations in domains before meiosis could facilitate 
      chromosome recognition and pairing in wheat.
PG  - 10597
LID - 10.1038/s41598-022-14843-1 [doi]
LID - 10597
AB  - The increasing human population demands an increase in crop yields that must be 
      implemented through breeding programmes to ensure a more efficient and 
      sustainable production of agro-food products. In the framework of breeding, 
      genetic crosses are developed between cultivated species such as wheat and their 
      relative species that are used as genetic donors to transfer desirable agronomic 
      traits into the crop. Unfortunately, interspecific associations between 
      chromosomes from the donor species and the cultivar are rare during meiosis, the 
      process to produce gametes in organisms with sexual reproduction, hampering the 
      transfer of genetic variability into wheat. In addition, little is known about 
      how homologous (equivalent) chromosomes initiate interaction and recognition 
      within the cell nucleus to enter meiosis. In this context, we aim to get insight 
      into wheat chromatin structure, particularly the distribution of homologous 
      chromosomes within the cell nucleus and their putative interactions in premeiotic 
      stages to facilitate chromosome associations and recombination at the beginning 
      of meiosis. Cytogenetics allows the study of both the structure and the behaviour 
      of chromosomes during meiosis and is key in plant breeding. In this study we 
      visualized an extra pair of barley homologous chromosomes in a wheat genetic 
      background to study the spatial distribution, arrangements and interactions 
      occurring exclusively between this pair of homologous chromosomes during 
      premeiosis using fluorescence in situ hybridization (FISH). Our results suggest 
      that homologous chromosomes can initiate interactions in premeiotic stages that 
      could facilitate the processes of specific chromosome recognition and association 
      occurring at the onset of meiosis.
CI  - (c) 2022. The Author(s).
FAU - Barea, Lorena
AU  - Barea L
AD  - Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal 
      Consejo Superior de Investigaciones Cientificas (CSIC), Avenida Menendez Pidal 
      S/N., Campus Alameda del Obispo, 14004, Cordoba, Spain.
FAU - Redondo-Rio, Alvaro
AU  - Redondo-Rio A
AD  - Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal 
      Consejo Superior de Investigaciones Cientificas (CSIC), Avenida Menendez Pidal 
      S/N., Campus Alameda del Obispo, 14004, Cordoba, Spain.
FAU - Lucena-Marin, Rafael
AU  - Lucena-Marin R
AD  - Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal 
      Consejo Superior de Investigaciones Cientificas (CSIC), Avenida Menendez Pidal 
      S/N., Campus Alameda del Obispo, 14004, Cordoba, Spain.
FAU - Serrano-Heredia, Salud
AU  - Serrano-Heredia S
AD  - Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal 
      Consejo Superior de Investigaciones Cientificas (CSIC), Avenida Menendez Pidal 
      S/N., Campus Alameda del Obispo, 14004, Cordoba, Spain.
FAU - Aguilar, Miguel
AU  - Aguilar M
AD  - Area de Fisiologia Vegetal, Universidad de Cordoba, Campus de Rabanales, Edif. 
      C4, 3 feminine Planta, Cordoba, Spain.
FAU - Prieto, Pilar
AU  - Prieto P
AUID- ORCID: 0000-0002-8160-808X
AD  - Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal 
      Consejo Superior de Investigaciones Cientificas (CSIC), Avenida Menendez Pidal 
      S/N., Campus Alameda del Obispo, 14004, Cordoba, Spain. pilar.prieto@ias.csic.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220622
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Chromosome Pairing/genetics
MH  - Chromosomes
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis/genetics
MH  - *Plant Breeding
MH  - *Triticum/genetics
PMC - PMC9217977
COIS- The authors declare no competing interests.
EDAT- 2022/06/23 06:00
MHDA- 2022/06/25 06:00
PMCR- 2022/06/22
CRDT- 2022/06/22 23:29
PHST- 2022/03/09 00:00 [received]
PHST- 2022/06/13 00:00 [accepted]
PHST- 2022/06/22 23:29 [entrez]
PHST- 2022/06/23 06:00 [pubmed]
PHST- 2022/06/25 06:00 [medline]
PHST- 2022/06/22 00:00 [pmc-release]
AID - 10.1038/s41598-022-14843-1 [pii]
AID - 14843 [pii]
AID - 10.1038/s41598-022-14843-1 [doi]
PST - epublish
SO  - Sci Rep. 2022 Jun 22;12(1):10597. doi: 10.1038/s41598-022-14843-1.