PMID- 35733046
OWN - NLM
STAT- MEDLINE
DCOM- 20220624
LR  - 20220726
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2492
DP  - 2022
TI  - Novel, Emerging Chip Models of the Blood-Brain Barrier and Future Directions.
PG  - 193-224
LID - 10.1007/978-1-0716-2289-6_11 [doi]
AB  - The use of microfluidic chips is now allowing for more advanced modelling of the 
      blood-brain barrier (BBB) in vitro, recapitulating heterotypic interactions, 3D 
      architecture, and physiological flow. This chapter will give an introduction to 
      these new technologies and how they are being applied to model the BBB and 
      neurovascular unit (NVU). A foundational understanding of the fluid dynamics 
      germane to the effective use of these chips will be set and an overview of how 
      physical phenomena at the microscale can be exploited to enable new possibilities 
      to control the cell culture environment. The four main approaches to construct 
      microfluidic blood vessel mimetics will be discussed with examples of how these 
      techniques are being applied to model the BBB and more recently to study specific 
      neurovascular disease processes. Finally, practical guidance will be given for 
      researchers wishing to adopt these new techniques along with a summary of the 
      challenges, limitations faced, and new opportunities opened up by these advanced 
      cell culture systems.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Holloway, Paul M
AU  - Holloway PM
AD  - Radcliffe Department of Medicine, University of Oxford, Oxford, UK. 
      paul.holloway@rdm.ox.ac.uk.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Biological Transport
MH  - *Blood-Brain Barrier
MH  - *Microfluidics/methods
OTO - NOTNLM
OT  - BBB
OT  - MPS
OT  - Microfluidics
OT  - Neurovascular unit
OT  - Organ-on-chip
EDAT- 2022/06/23 06:00
MHDA- 2022/06/25 06:00
CRDT- 2022/06/22 23:34
PHST- 2022/06/22 23:34 [entrez]
PHST- 2022/06/23 06:00 [pubmed]
PHST- 2022/06/25 06:00 [medline]
AID - 10.1007/978-1-0716-2289-6_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2492:193-224. doi: 10.1007/978-1-0716-2289-6_11.